Development of a quantitative sandwich enzyme-linked immunosorbent assay for detecting the MPT64 antigen of mycobacterium tuberculosis

Mijung Ji, Byungki Cho, Young Shik Cho, Song Yong Park, Sangnae Cho, Bo Young Jeon, Byoung Su Yoon

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Purpose: Tuberculosis (TB) is a major infectious disease and is responsible for two million deaths annually. For the identification and quantitation of Mycobacterium tuberculosis (M. tuberculosis), a causative agent of TB, a sandwich enzyme-linked immunosorbent assay (ELISA) against the MPT64 protein of M. tuberculosis, an antigen marker of the M. tuberculosis complex, was developed. Materials and Methods: The MPT64 protein was expressed, and anti-MPT64 monoclonal antibodies were prepared. A sandwich ELISA was established using recombinant MPT64 protein and anti-MPT64 monoclonal antibodies. The sandwich MPT64 ELISA was evaluated using reference and clinical mycobacterial strains. Results: The sandwich MPT64 ELISA detected MPT64 protein from 2.1 ng/mL to 250 ng/mL (equivalent to 1.7×104 CFU/mL and 2.0×106 CFU/mL). All 389 clinical M. tuberculosis isolates tested positive in the sandwich MPT64 ELISA (sensitivity, 100%), and the assay showed no cross reactivity to any tested nontuberculous mycobacterial strain (specificity, 100%). Conclusion: The sandwich MPT64 ELISA is a highly sensitive and quantitative test for MPT64 protein, which can identify M. tuberculosis.

Original languageEnglish
Pages (from-to)746-752
Number of pages7
JournalYonsei medical journal
Volume55
Issue number3
DOIs
Publication statusPublished - 2014 Jan 1

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Enzyme-Linked Immunosorbent Assay
Mycobacterium tuberculosis
Tuberculosis
Proteins
Monoclonal Antibodies
Recombinant Proteins
Communicable Diseases
Mycobacterium tuberculosis antigens

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Ji, Mijung ; Cho, Byungki ; Cho, Young Shik ; Park, Song Yong ; Cho, Sangnae ; Jeon, Bo Young ; Yoon, Byoung Su. / Development of a quantitative sandwich enzyme-linked immunosorbent assay for detecting the MPT64 antigen of mycobacterium tuberculosis. In: Yonsei medical journal. 2014 ; Vol. 55, No. 3. pp. 746-752.
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Development of a quantitative sandwich enzyme-linked immunosorbent assay for detecting the MPT64 antigen of mycobacterium tuberculosis. / Ji, Mijung; Cho, Byungki; Cho, Young Shik; Park, Song Yong; Cho, Sangnae; Jeon, Bo Young; Yoon, Byoung Su.

In: Yonsei medical journal, Vol. 55, No. 3, 01.01.2014, p. 746-752.

Research output: Contribution to journalArticle

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