Development of a small animal model to simulate clinical stereotactic body radiotherapy-induced central and peripheral lung injuries

Zhen Yu Hong, Sung Ho Eun, Kwangwoo Park, Won Hoon Choi, Jung Il Lee, Eun Jung Lee, Ji Min Lee, Michael D. Story, Jaeho Cho

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Given the tremendous potential of stereotactic body radiotherapy (SBRT), investigations of the underlying radiobiology associated with SBRT-induced normal tissue injury are of paramount importance. This study was designed to develop an animal model that simulates centrally and peripherally located clinical SBRT-induced lung injuries. A 90-Gy irradiation dose was focally delivered to the central and peripheral areas of the left mouse lung with an image-guided small-animal irradiation system. At 1, 2 and 4 weeks after irradiation, micro-computed tomography (micro-CT) images of the lung were taken. Lung function measurements were performed with the Flexivent ® system (SCIREQ © , Montreal, Canada). For the histopathological analysis, the lungs were fixed by perfusing with formalin, and paraffin sections were stained with hematoxylin and eosin and Masson's Trichrome. Gross inspection clearly indicated local lung injury confined to the central and peripheral areas of the left lung. Typical histopathological alterations corresponding to clinical manifestations were observed. The micro-CT analysis results appeared to correlate with the histopathological findings. Mouse lung tissue damping increased dramatically at central settings, compared with that at the control or peripheral settings. An animal model to simulate clinical SBRT-induced central and peripheral lung injuries was developed and validated with histopathological, radiological and functional analyses. This model increases our understanding of SBRT-induced central and peripheral lung injuries and will help to improve radiation therapy in the future.

Original languageEnglish
Pages (from-to)648-657
Number of pages10
JournalJournal of radiation research
Volume55
Issue number4
DOIs
Publication statusPublished - 2014 Jul

Fingerprint

animal models
Radiosurgery
Lung Injury
lungs
radiation therapy
Animal Models
Lung
Tomography
Radiobiology
irradiation
mice
Hematoxylin
Eosine Yellowish-(YS)
Paraffin
Formaldehyde
tomography
Canada
radiobiology
Radiotherapy
paraffins

All Science Journal Classification (ASJC) codes

  • Radiation
  • Radiology Nuclear Medicine and imaging
  • Health, Toxicology and Mutagenesis

Cite this

Hong, Zhen Yu ; Eun, Sung Ho ; Park, Kwangwoo ; Choi, Won Hoon ; Lee, Jung Il ; Lee, Eun Jung ; Lee, Ji Min ; Story, Michael D. ; Cho, Jaeho. / Development of a small animal model to simulate clinical stereotactic body radiotherapy-induced central and peripheral lung injuries. In: Journal of radiation research. 2014 ; Vol. 55, No. 4. pp. 648-657.
@article{36c0916e18c74ebfa7bf57ce36a8cc7e,
title = "Development of a small animal model to simulate clinical stereotactic body radiotherapy-induced central and peripheral lung injuries",
abstract = "Given the tremendous potential of stereotactic body radiotherapy (SBRT), investigations of the underlying radiobiology associated with SBRT-induced normal tissue injury are of paramount importance. This study was designed to develop an animal model that simulates centrally and peripherally located clinical SBRT-induced lung injuries. A 90-Gy irradiation dose was focally delivered to the central and peripheral areas of the left mouse lung with an image-guided small-animal irradiation system. At 1, 2 and 4 weeks after irradiation, micro-computed tomography (micro-CT) images of the lung were taken. Lung function measurements were performed with the Flexivent {\circledR} system (SCIREQ {\circledC} , Montreal, Canada). For the histopathological analysis, the lungs were fixed by perfusing with formalin, and paraffin sections were stained with hematoxylin and eosin and Masson's Trichrome. Gross inspection clearly indicated local lung injury confined to the central and peripheral areas of the left lung. Typical histopathological alterations corresponding to clinical manifestations were observed. The micro-CT analysis results appeared to correlate with the histopathological findings. Mouse lung tissue damping increased dramatically at central settings, compared with that at the control or peripheral settings. An animal model to simulate clinical SBRT-induced central and peripheral lung injuries was developed and validated with histopathological, radiological and functional analyses. This model increases our understanding of SBRT-induced central and peripheral lung injuries and will help to improve radiation therapy in the future.",
author = "Hong, {Zhen Yu} and Eun, {Sung Ho} and Kwangwoo Park and Choi, {Won Hoon} and Lee, {Jung Il} and Lee, {Eun Jung} and Lee, {Ji Min} and Story, {Michael D.} and Jaeho Cho",
year = "2014",
month = "7",
doi = "10.1093/jrr/rrt234",
language = "English",
volume = "55",
pages = "648--657",
journal = "Journal of Radiation Research",
issn = "0449-3060",
publisher = "Japan Radiation Research Society",
number = "4",

}

Development of a small animal model to simulate clinical stereotactic body radiotherapy-induced central and peripheral lung injuries. / Hong, Zhen Yu; Eun, Sung Ho; Park, Kwangwoo; Choi, Won Hoon; Lee, Jung Il; Lee, Eun Jung; Lee, Ji Min; Story, Michael D.; Cho, Jaeho.

In: Journal of radiation research, Vol. 55, No. 4, 07.2014, p. 648-657.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Development of a small animal model to simulate clinical stereotactic body radiotherapy-induced central and peripheral lung injuries

AU - Hong, Zhen Yu

AU - Eun, Sung Ho

AU - Park, Kwangwoo

AU - Choi, Won Hoon

AU - Lee, Jung Il

AU - Lee, Eun Jung

AU - Lee, Ji Min

AU - Story, Michael D.

AU - Cho, Jaeho

PY - 2014/7

Y1 - 2014/7

N2 - Given the tremendous potential of stereotactic body radiotherapy (SBRT), investigations of the underlying radiobiology associated with SBRT-induced normal tissue injury are of paramount importance. This study was designed to develop an animal model that simulates centrally and peripherally located clinical SBRT-induced lung injuries. A 90-Gy irradiation dose was focally delivered to the central and peripheral areas of the left mouse lung with an image-guided small-animal irradiation system. At 1, 2 and 4 weeks after irradiation, micro-computed tomography (micro-CT) images of the lung were taken. Lung function measurements were performed with the Flexivent ® system (SCIREQ © , Montreal, Canada). For the histopathological analysis, the lungs were fixed by perfusing with formalin, and paraffin sections were stained with hematoxylin and eosin and Masson's Trichrome. Gross inspection clearly indicated local lung injury confined to the central and peripheral areas of the left lung. Typical histopathological alterations corresponding to clinical manifestations were observed. The micro-CT analysis results appeared to correlate with the histopathological findings. Mouse lung tissue damping increased dramatically at central settings, compared with that at the control or peripheral settings. An animal model to simulate clinical SBRT-induced central and peripheral lung injuries was developed and validated with histopathological, radiological and functional analyses. This model increases our understanding of SBRT-induced central and peripheral lung injuries and will help to improve radiation therapy in the future.

AB - Given the tremendous potential of stereotactic body radiotherapy (SBRT), investigations of the underlying radiobiology associated with SBRT-induced normal tissue injury are of paramount importance. This study was designed to develop an animal model that simulates centrally and peripherally located clinical SBRT-induced lung injuries. A 90-Gy irradiation dose was focally delivered to the central and peripheral areas of the left mouse lung with an image-guided small-animal irradiation system. At 1, 2 and 4 weeks after irradiation, micro-computed tomography (micro-CT) images of the lung were taken. Lung function measurements were performed with the Flexivent ® system (SCIREQ © , Montreal, Canada). For the histopathological analysis, the lungs were fixed by perfusing with formalin, and paraffin sections were stained with hematoxylin and eosin and Masson's Trichrome. Gross inspection clearly indicated local lung injury confined to the central and peripheral areas of the left lung. Typical histopathological alterations corresponding to clinical manifestations were observed. The micro-CT analysis results appeared to correlate with the histopathological findings. Mouse lung tissue damping increased dramatically at central settings, compared with that at the control or peripheral settings. An animal model to simulate clinical SBRT-induced central and peripheral lung injuries was developed and validated with histopathological, radiological and functional analyses. This model increases our understanding of SBRT-induced central and peripheral lung injuries and will help to improve radiation therapy in the future.

UR - http://www.scopus.com/inward/record.url?scp=84905269826&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84905269826&partnerID=8YFLogxK

U2 - 10.1093/jrr/rrt234

DO - 10.1093/jrr/rrt234

M3 - Article

C2 - 24556815

AN - SCOPUS:84905269826

VL - 55

SP - 648

EP - 657

JO - Journal of Radiation Research

JF - Journal of Radiation Research

SN - 0449-3060

IS - 4

ER -