The emergence and spread of antiviral drug-resistant viruses have been a worldwide challenge and a great concern for patient care. We report A4 antibody specifically recognizing and binding to the mutant I223R/H275Y neuraminidase and prove the applicability of A4 antibody for direct detection of antiviral multidrug-resistant viruses in various sensing platforms, including naked-eye detection, surface-enhanced Raman scattering-based immunoassay, and lateral flow system. The development of the A4 antibody enables fast, simple, and reliable point-of-care assays of antiviral multidrug-resistant influenza viruses. In addition to current influenza virus infection testing methods that do not provide information on the antiviral drug-resistance of the virus, diagnostic tests for antiviral multidrug-resistant viruses will improve clinical judgment in the treatment of influenza virus infections, avoid the unnecessary prescription of ineffective drugs, and improve current therapies.
Bibliographical noteFunding Information:
This research was supported by the Center for BioNano Health-Guard funded by the Ministry of Science and ICT (MSIT) of Korea as Global Frontier Project (HGUARD_2013M3A6B2078950 and HGUARD_2014M3A6B2060507), the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by MSIT of Korea (NRF-2018M3A9E2022821), the Basic Science Research Program of the NRF funded by MSIT of Korea (NRF-2018R1C1B6005424, NRF-2020R1A2C1010453 and NRF2019R1C1C1006867), and KRIBB Initiative Research Program.
All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)
- Physics and Astronomy(all)