Development of A4 antibody for detection of neuraminidase I223R/H275Y-associated antiviral multidrug-resistant influenza virus

Kyeonghye Guk, Hyeran Kim, Miyeon Lee, Yoon Aa Choi, Seul Gee Hwang, Gaon Han, Hye Nan Kim, Hongki Kim, Hwangseo Park, Dongeun Yong, Taejoon Kang, Eun Kyung Lim, Juyeon Jung

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1 Citation (Scopus)

Abstract

The emergence and spread of antiviral drug-resistant viruses have been a worldwide challenge and a great concern for patient care. We report A4 antibody specifically recognizing and binding to the mutant I223R/H275Y neuraminidase and prove the applicability of A4 antibody for direct detection of antiviral multidrug-resistant viruses in various sensing platforms, including naked-eye detection, surface-enhanced Raman scattering-based immunoassay, and lateral flow system. The development of the A4 antibody enables fast, simple, and reliable point-of-care assays of antiviral multidrug-resistant influenza viruses. In addition to current influenza virus infection testing methods that do not provide information on the antiviral drug-resistance of the virus, diagnostic tests for antiviral multidrug-resistant viruses will improve clinical judgment in the treatment of influenza virus infections, avoid the unnecessary prescription of ineffective drugs, and improve current therapies.

Original languageEnglish
Article number3418
JournalNature communications
Volume11
Issue number1
DOIs
Publication statusPublished - 2020 Dec 1

Bibliographical note

Funding Information:
This research was supported by the Center for BioNano Health-Guard funded by the Ministry of Science and ICT (MSIT) of Korea as Global Frontier Project (HGUARD_2013M3A6B2078950 and HGUARD_2014M3A6B2060507), the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by MSIT of Korea (NRF-2018M3A9E2022821), the Basic Science Research Program of the NRF funded by MSIT of Korea (NRF-2018R1C1B6005424, NRF-2020R1A2C1010453 and NRF2019R1C1C1006867), and KRIBB Initiative Research Program.

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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