Development of allergic conjunctivitis induced by house dust mite extract from dermatophagoides pteronyssinus

Young Ji Lee, Soo Jung Han, Hun Lee, Jin Sun Kim, KyoungYul Seo

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Purpose. The purpose of this study was to develop a murine model of allergic conjunctivitis induced by house dust mite (HDM) extract fromDermatophagoides pteronyssinus, a major allergen in humans. Methods. Forty BALB/c mice were divided into five groups, immunized with placebo, ovalbumin (10 μg), or HDM extract following a schedule. Twenty minutes after topical challenge, mice were examined clinically. Material collected from mice was used for measuring total and specific IgE, antigen-specific lymphocyte proliferation, and supernatant cytokine levels and for conjunctival histopathology and flow cytometric analysis of conjunctival cells. Results. This murine model showed similar clinical signs and laboratory findings to human allergy and the ovalbumin-induced allergic conjunctivitis model. Total IgE levels and conjunctival infiltration of mast cells and eosinophils in immunized mice were significantly higher than in the control group. Cervical lymphocyte proliferation was increased in antigen-stimulated cultures in immunized mice, concomitant with significantly higher IL-4 and IL-5 levels in the culture supernatant. The proportion of conjunctival CD4 + T cells expressing the ST2 receptor was increased, and conjunctival CD4 + ST2 + T cells exhibited an increase in intracellular IL-5. Conclusions. House dust mite extract successfully induced allergic conjunctivitis in BALB/c mice. Ten micrograms of HDM extract was the optimal dose for systemic immunization in this model. This murine model is suitable for further studies on HDM-induced allergic conjunctivitis, and the data show that conjunctival CD4 + T cells expressing ST2 may play an important role in IL-5 secretion, recruiting eosinophils into conjunctiva on ocular allergen challenge.

Original languageEnglish
Pages (from-to)1773-1781
Number of pages9
JournalInvestigative Ophthalmology and Visual Science
Volume57
Issue number4
DOIs
Publication statusPublished - 2016 Apr 1

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Dermatophagoides pteronyssinus
Allergic Conjunctivitis
Pyroglyphidae
Interleukin-5
Ovalbumin
T-Lymphocytes
Eosinophils
Allergens
Immunoglobulin E
Lymphocytes
Antigens
Conjunctiva
Mast Cells
Interleukin-4
Immunization
Appointments and Schedules
Hypersensitivity
Placebos
Cytokines
Control Groups

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

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title = "Development of allergic conjunctivitis induced by house dust mite extract from dermatophagoides pteronyssinus",
abstract = "Purpose. The purpose of this study was to develop a murine model of allergic conjunctivitis induced by house dust mite (HDM) extract fromDermatophagoides pteronyssinus, a major allergen in humans. Methods. Forty BALB/c mice were divided into five groups, immunized with placebo, ovalbumin (10 μg), or HDM extract following a schedule. Twenty minutes after topical challenge, mice were examined clinically. Material collected from mice was used for measuring total and specific IgE, antigen-specific lymphocyte proliferation, and supernatant cytokine levels and for conjunctival histopathology and flow cytometric analysis of conjunctival cells. Results. This murine model showed similar clinical signs and laboratory findings to human allergy and the ovalbumin-induced allergic conjunctivitis model. Total IgE levels and conjunctival infiltration of mast cells and eosinophils in immunized mice were significantly higher than in the control group. Cervical lymphocyte proliferation was increased in antigen-stimulated cultures in immunized mice, concomitant with significantly higher IL-4 and IL-5 levels in the culture supernatant. The proportion of conjunctival CD4 + T cells expressing the ST2 receptor was increased, and conjunctival CD4 + ST2 + T cells exhibited an increase in intracellular IL-5. Conclusions. House dust mite extract successfully induced allergic conjunctivitis in BALB/c mice. Ten micrograms of HDM extract was the optimal dose for systemic immunization in this model. This murine model is suitable for further studies on HDM-induced allergic conjunctivitis, and the data show that conjunctival CD4 + T cells expressing ST2 may play an important role in IL-5 secretion, recruiting eosinophils into conjunctiva on ocular allergen challenge.",
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Development of allergic conjunctivitis induced by house dust mite extract from dermatophagoides pteronyssinus. / Lee, Young Ji; Han, Soo Jung; Lee, Hun; Kim, Jin Sun; Seo, KyoungYul.

In: Investigative Ophthalmology and Visual Science, Vol. 57, No. 4, 01.04.2016, p. 1773-1781.

Research output: Contribution to journalArticle

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T1 - Development of allergic conjunctivitis induced by house dust mite extract from dermatophagoides pteronyssinus

AU - Lee, Young Ji

AU - Han, Soo Jung

AU - Lee, Hun

AU - Kim, Jin Sun

AU - Seo, KyoungYul

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N2 - Purpose. The purpose of this study was to develop a murine model of allergic conjunctivitis induced by house dust mite (HDM) extract fromDermatophagoides pteronyssinus, a major allergen in humans. Methods. Forty BALB/c mice were divided into five groups, immunized with placebo, ovalbumin (10 μg), or HDM extract following a schedule. Twenty minutes after topical challenge, mice were examined clinically. Material collected from mice was used for measuring total and specific IgE, antigen-specific lymphocyte proliferation, and supernatant cytokine levels and for conjunctival histopathology and flow cytometric analysis of conjunctival cells. Results. This murine model showed similar clinical signs and laboratory findings to human allergy and the ovalbumin-induced allergic conjunctivitis model. Total IgE levels and conjunctival infiltration of mast cells and eosinophils in immunized mice were significantly higher than in the control group. Cervical lymphocyte proliferation was increased in antigen-stimulated cultures in immunized mice, concomitant with significantly higher IL-4 and IL-5 levels in the culture supernatant. The proportion of conjunctival CD4 + T cells expressing the ST2 receptor was increased, and conjunctival CD4 + ST2 + T cells exhibited an increase in intracellular IL-5. Conclusions. House dust mite extract successfully induced allergic conjunctivitis in BALB/c mice. Ten micrograms of HDM extract was the optimal dose for systemic immunization in this model. This murine model is suitable for further studies on HDM-induced allergic conjunctivitis, and the data show that conjunctival CD4 + T cells expressing ST2 may play an important role in IL-5 secretion, recruiting eosinophils into conjunctiva on ocular allergen challenge.

AB - Purpose. The purpose of this study was to develop a murine model of allergic conjunctivitis induced by house dust mite (HDM) extract fromDermatophagoides pteronyssinus, a major allergen in humans. Methods. Forty BALB/c mice were divided into five groups, immunized with placebo, ovalbumin (10 μg), or HDM extract following a schedule. Twenty minutes after topical challenge, mice were examined clinically. Material collected from mice was used for measuring total and specific IgE, antigen-specific lymphocyte proliferation, and supernatant cytokine levels and for conjunctival histopathology and flow cytometric analysis of conjunctival cells. Results. This murine model showed similar clinical signs and laboratory findings to human allergy and the ovalbumin-induced allergic conjunctivitis model. Total IgE levels and conjunctival infiltration of mast cells and eosinophils in immunized mice were significantly higher than in the control group. Cervical lymphocyte proliferation was increased in antigen-stimulated cultures in immunized mice, concomitant with significantly higher IL-4 and IL-5 levels in the culture supernatant. The proportion of conjunctival CD4 + T cells expressing the ST2 receptor was increased, and conjunctival CD4 + ST2 + T cells exhibited an increase in intracellular IL-5. Conclusions. House dust mite extract successfully induced allergic conjunctivitis in BALB/c mice. Ten micrograms of HDM extract was the optimal dose for systemic immunization in this model. This murine model is suitable for further studies on HDM-induced allergic conjunctivitis, and the data show that conjunctival CD4 + T cells expressing ST2 may play an important role in IL-5 secretion, recruiting eosinophils into conjunctiva on ocular allergen challenge.

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