Development of an angiogenesis-focused cDNA chip and validation of its functionality

Joong Sup Shim, Hae Kwang Lee, Hyo Mi Park, Jong Ok Kim, Eun Kyung Kim, Kyung Hoon Hwang, Ki Tae Kim, Seong Whan Park, Je Hyeon Lee, Ho Jeong Kwon

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

DNA chip has been used as a powerful tool to study the genetic reprogramming of cells and its link to cellular phenotype such as angiogenesis. To evaluate the angiogenesis related genetic reprogramming more efficiently, we here developed an angiogenesis-focused cDNA chip containing 153 angiogenesis related genes arrayed in duplicate on a slide glass. In order to validate the functionality of the angiogenesis-focused cDNA chip, we examined gene expression profiles in HT1080 cells treated with either fetal bovine serum, a well known pro-angiogenic factor, or trichostatin A, a known angiogenesis inhibitor, using the cDNA chip. All duplicate data from the analysis are well matched with each other and gene expression profiles are well consistent with previously reported data. These results demonstrate that the angiogenesis-focused cDNA chip developed here can be a useful tool towards angiogenesis-related researches.

Original languageEnglish
Pages (from-to)365-370
Number of pages6
JournalExperimental and Molecular Medicine
Volume37
Issue number4
DOIs
Publication statusPublished - 2005 Aug 31

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

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  • Cite this

    Shim, J. S., Lee, H. K., Park, H. M., Kim, J. O., Kim, E. K., Hwang, K. H., Kim, K. T., Park, S. W., Lee, J. H., & Kwon, H. J. (2005). Development of an angiogenesis-focused cDNA chip and validation of its functionality. Experimental and Molecular Medicine, 37(4), 365-370. https://doi.org/10.1038/emm.2005.46