Development of analytic microdevices for the detection of phenol using polymer hydrogel particles containing enzyme-QD conjugates

Sun A. Park, Eunji Jang, Won-Gun Koh, Bumsang Kim

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We present the fabrication of a microdevice for the detection of phenol by combining microfluidic channels and poly(2-hydroxyethyl methacrylate) (PHEMA) hydrogel microparticles containing tyrosinase-quantum dot conjugates. PHEMA hydrogel microparticles containing conjugates of enzyme (tyrosinase) and quantum dot (QD) were prepared by dispersion photopolymerization and entrapped within a microfilter-incorporated reaction chamber in a microfluidic channel. The fluorescence change, due to the fluorescence quenching effect caused by the enzyme reaction between phenol and tyrosinase, was used to detect phenol. The fluorescence intensity of PHEMA hydrogel microparticles containing tyrosinase-QD conjugates at 585nm decreased with phenol concentration. In conclusion, the microfluidic channels fabricated in this study entrapping PHEMA hydrogel microparticles containing enzyme-QD conjugates show the potential to be used as an analytic microdevice for the detection of phenol.

Original languageEnglish
Pages (from-to)1000-1003
Number of pages4
JournalTalanta
Volume84
Issue number3
DOIs
Publication statusPublished - 2011 May 15

Fingerprint

Quantum Dots
Hydrogel
Phenol
Monophenol Monooxygenase
Semiconductor quantum dots
Polymers
Microfluidics
Enzymes
Fluorescence
Photopolymerization
Quenching
Fabrication
hydroxyethyl methacrylate

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Chemistry(all)
  • Biochemistry
  • Spectroscopy

Cite this

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abstract = "We present the fabrication of a microdevice for the detection of phenol by combining microfluidic channels and poly(2-hydroxyethyl methacrylate) (PHEMA) hydrogel microparticles containing tyrosinase-quantum dot conjugates. PHEMA hydrogel microparticles containing conjugates of enzyme (tyrosinase) and quantum dot (QD) were prepared by dispersion photopolymerization and entrapped within a microfilter-incorporated reaction chamber in a microfluidic channel. The fluorescence change, due to the fluorescence quenching effect caused by the enzyme reaction between phenol and tyrosinase, was used to detect phenol. The fluorescence intensity of PHEMA hydrogel microparticles containing tyrosinase-QD conjugates at 585nm decreased with phenol concentration. In conclusion, the microfluidic channels fabricated in this study entrapping PHEMA hydrogel microparticles containing enzyme-QD conjugates show the potential to be used as an analytic microdevice for the detection of phenol.",
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Development of analytic microdevices for the detection of phenol using polymer hydrogel particles containing enzyme-QD conjugates. / Park, Sun A.; Jang, Eunji; Koh, Won-Gun; Kim, Bumsang.

In: Talanta, Vol. 84, No. 3, 15.05.2011, p. 1000-1003.

Research output: Contribution to journalArticle

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AB - We present the fabrication of a microdevice for the detection of phenol by combining microfluidic channels and poly(2-hydroxyethyl methacrylate) (PHEMA) hydrogel microparticles containing tyrosinase-quantum dot conjugates. PHEMA hydrogel microparticles containing conjugates of enzyme (tyrosinase) and quantum dot (QD) were prepared by dispersion photopolymerization and entrapped within a microfilter-incorporated reaction chamber in a microfluidic channel. The fluorescence change, due to the fluorescence quenching effect caused by the enzyme reaction between phenol and tyrosinase, was used to detect phenol. The fluorescence intensity of PHEMA hydrogel microparticles containing tyrosinase-QD conjugates at 585nm decreased with phenol concentration. In conclusion, the microfluidic channels fabricated in this study entrapping PHEMA hydrogel microparticles containing enzyme-QD conjugates show the potential to be used as an analytic microdevice for the detection of phenol.

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