Development of effective modified cellulase for cellulose hydrolysis process

Jin Won Park, Toshio Kajiuchi

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Cellulase was modified with amphilic copolymers made of α‐allyl‐ω‐methoxy polyoxyalkylene (POA) and maleic acid anhydride (MAA) to improve the cellulose hydrolytic reactivity and cellulase separation. Amino groups of the cellulase molecule are covalently coupled with the MAA functional groups of the copolymer. At the maximum degree of modification (DM) of 55%, the modified cellulase activity retained more than 80% of the unmodified native cellulase activity. The modified cellulase shows greater stability against temperature, pH, and organic solvents, and demonstrated greater conversion of substrate than native cellulase does. Cellulase modification is also useful for controlling strong adsorption of cellulase onto substrate. Moreover, cellulase modified with the amphiphilic copolymer displays different separation characteristics which are new. One is a reactive two‐phase partition and another is solubility in organic solvents. It appears that these characteristics of modified cellulase work very effectively in the hydrolysis of cellulose as a total system, which constitutes the purification of cellulase from culture broth, hydrolysis of cellulose, and recovery of cellulase from the reaction mixture. © 1995 John Wiley & Sons, Inc.

Original languageEnglish
Pages (from-to)366-373
Number of pages8
JournalBiotechnology and Bioengineering
Volume45
Issue number4
DOIs
Publication statusPublished - 1995 Jan 1

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Cellulase
Cellulose
Hydrolysis
Copolymers
Organic solvents
Acids
Substrates
Functional groups
Purification
Solubility
Maleic Anhydrides
Adsorption
Recovery
Molecules
Anhydrides
Temperature

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

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abstract = "Cellulase was modified with amphilic copolymers made of α‐allyl‐ω‐methoxy polyoxyalkylene (POA) and maleic acid anhydride (MAA) to improve the cellulose hydrolytic reactivity and cellulase separation. Amino groups of the cellulase molecule are covalently coupled with the MAA functional groups of the copolymer. At the maximum degree of modification (DM) of 55{\%}, the modified cellulase activity retained more than 80{\%} of the unmodified native cellulase activity. The modified cellulase shows greater stability against temperature, pH, and organic solvents, and demonstrated greater conversion of substrate than native cellulase does. Cellulase modification is also useful for controlling strong adsorption of cellulase onto substrate. Moreover, cellulase modified with the amphiphilic copolymer displays different separation characteristics which are new. One is a reactive two‐phase partition and another is solubility in organic solvents. It appears that these characteristics of modified cellulase work very effectively in the hydrolysis of cellulose as a total system, which constitutes the purification of cellulase from culture broth, hydrolysis of cellulose, and recovery of cellulase from the reaction mixture. {\circledC} 1995 John Wiley & Sons, Inc.",
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Development of effective modified cellulase for cellulose hydrolysis process. / Park, Jin Won; Kajiuchi, Toshio.

In: Biotechnology and Bioengineering, Vol. 45, No. 4, 01.01.1995, p. 366-373.

Research output: Contribution to journalArticle

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AB - Cellulase was modified with amphilic copolymers made of α‐allyl‐ω‐methoxy polyoxyalkylene (POA) and maleic acid anhydride (MAA) to improve the cellulose hydrolytic reactivity and cellulase separation. Amino groups of the cellulase molecule are covalently coupled with the MAA functional groups of the copolymer. At the maximum degree of modification (DM) of 55%, the modified cellulase activity retained more than 80% of the unmodified native cellulase activity. The modified cellulase shows greater stability against temperature, pH, and organic solvents, and demonstrated greater conversion of substrate than native cellulase does. Cellulase modification is also useful for controlling strong adsorption of cellulase onto substrate. Moreover, cellulase modified with the amphiphilic copolymer displays different separation characteristics which are new. One is a reactive two‐phase partition and another is solubility in organic solvents. It appears that these characteristics of modified cellulase work very effectively in the hydrolysis of cellulose as a total system, which constitutes the purification of cellulase from culture broth, hydrolysis of cellulose, and recovery of cellulase from the reaction mixture. © 1995 John Wiley & Sons, Inc.

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