Development of folate-bound intracellular pH-sensitive polymeric micelle drug carriers for tumor-directed cancer treatment

Younsoo Bae, Woo Dong Jang, Nobuhiro Nishiyama, Kazunori Kataoka

Research output: Contribution to conferencePaper

Abstract

A new type of multi-functional intracellular pH-sensitive polymeric micelle carrier system for active drug delivery was prepared and characterized in this study. Self-assembling amphiphilic block copolymers, folate-pory(ethylene glycol)-poly (aspartate hydrazone adriamycin) [Fol-PEG-P(Asp-Hyd-ADR)], were synthesized to prepare the micelles, which have approximately 60 nm diameters. A folate molecule was conjugated to the end of the shell-forming PEG chain while anticancer drugs, adriamycin were bound to the side chain of the core-forming segment through an acid-sensitive hydrazone bond that is selectively cleavable under intracellular acidic environment (pH 5-6). The selectivity of the micelles to folate-binding proteins was evaluated by surface plasmon resonance measurements, and MTT assay, using human pharyngeal cancer cell (KB cell), revealed that the micelles improved cell growth inhibitory activity significantly in spite of a short exposure time probably due to the selective and strong interaction between folate molecules and their receptors.

Original languageEnglish
Number of pages1
Publication statusPublished - 2005 Dec 1
Event54th SPSJ Annual Meeting 2005 - Yokohama, Japan
Duration: 2005 May 252005 May 27

Other

Other54th SPSJ Annual Meeting 2005
CountryJapan
CityYokohama
Period05/5/2505/5/27

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Oncology
Micelles
Tumors
Polyethylene glycols
Molecules
Cell growth
Surface plasmon resonance
Ethylene glycol
Drug delivery
Block copolymers
Assays
Cells
Acids

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Bae, Y., Jang, W. D., Nishiyama, N., & Kataoka, K. (2005). Development of folate-bound intracellular pH-sensitive polymeric micelle drug carriers for tumor-directed cancer treatment. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.
Bae, Younsoo ; Jang, Woo Dong ; Nishiyama, Nobuhiro ; Kataoka, Kazunori. / Development of folate-bound intracellular pH-sensitive polymeric micelle drug carriers for tumor-directed cancer treatment. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.1 p.
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Bae, Y, Jang, WD, Nishiyama, N & Kataoka, K 2005, 'Development of folate-bound intracellular pH-sensitive polymeric micelle drug carriers for tumor-directed cancer treatment' Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan, 05/5/25 - 05/5/27, .

Development of folate-bound intracellular pH-sensitive polymeric micelle drug carriers for tumor-directed cancer treatment. / Bae, Younsoo; Jang, Woo Dong; Nishiyama, Nobuhiro; Kataoka, Kazunori.

2005. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.

Research output: Contribution to conferencePaper

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AU - Kataoka, Kazunori

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Bae Y, Jang WD, Nishiyama N, Kataoka K. Development of folate-bound intracellular pH-sensitive polymeric micelle drug carriers for tumor-directed cancer treatment. 2005. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.