Development of folate-bound intracellular pH-sensitive polymeric micelle drug carriers for tumor-directed cancer treatment

Younsoo Bae, Woo Dong Jang, Nobuhiro Nishiyama, Kazunori Kataoka

Research output: Contribution to conferencePaperpeer-review

Abstract

A new type of multi-functional intracellular pH-sensitive polymeric micelle carrier system for active drug delivery was prepared and characterized in this study. Self-assembling amphiphilic block copolymers, folate-pory(ethylene glycol)-poly (aspartate hydrazone adriamycin) [Fol-PEG-P(Asp-Hyd-ADR)], were synthesized to prepare the micelles, which have approximately 60 nm diameters. A folate molecule was conjugated to the end of the shell-forming PEG chain while anticancer drugs, adriamycin were bound to the side chain of the core-forming segment through an acid-sensitive hydrazone bond that is selectively cleavable under intracellular acidic environment (pH 5-6). The selectivity of the micelles to folate-binding proteins was evaluated by surface plasmon resonance measurements, and MTT assay, using human pharyngeal cancer cell (KB cell), revealed that the micelles improved cell growth inhibitory activity significantly in spite of a short exposure time probably due to the selective and strong interaction between folate molecules and their receptors.

Original languageEnglish
Pages2190
Number of pages1
Publication statusPublished - 2005
Event54th SPSJ Annual Meeting 2005 - Yokohama, Japan
Duration: 2005 May 252005 May 27

Other

Other54th SPSJ Annual Meeting 2005
CountryJapan
CityYokohama
Period05/5/2505/5/27

All Science Journal Classification (ASJC) codes

  • Engineering(all)

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