Development of isotope dilution lc-ms/ms method for accurate determination of arsenobetaine in oyster certified reference material

Woo Young Lee, Yong Hyeon Yim, Euijin Hwang, Youngran Lim, Tae Kyu Kim, Kyoung Seok Lee

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An isotope dilution liquid chromatography tandem mass spectrometry (ID LC-MS/MS) method has been developed and applied to the determination of arsenobetaine (AsB, (CH3)3 +AsCH 2COO-) from oyster candidate certified reference material (CRM). The exact matching isotope dilution approach was adopted for accurate determination of AsB using 13C2-labeled AsB as an internal standard. Efficiencies of different AsB extraction methods were evaluated using a codfish reference material and a simple sonication method was selected as the method of choice for the certification of the oyster candidate CRM. The hydrophilic interaction liquid chromatography (HILIC) combined with electrospray ionization tandem mass spectrometry (ESI/MS/MS) in selected reaction monitoring (SRM) mode was optimized for adequate chromatographic retention and robust quantification of AsB from codfish and oyster samples. By analyzing 12 subsamples taken from each 12 bottles systematically selected from the whole oyster CRM batch, the certified value of AsB was determined as 6.60 mg·kg-1 ± 0.31 mg·kg-1 and it showed excellent between-bottle homogeneity of less than 0.42%, which is represented by relative standard deviation of 12 bottles from the CRM batch. The major source of uncertainty was the certified value of the AsB standard solution.

Original languageEnglish
Pages (from-to)821-827
Number of pages7
JournalBulletin of the Korean Chemical Society
Issue number3
Publication statusPublished - 2014 Mar 20


All Science Journal Classification (ASJC) codes

  • Chemistry(all)

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