Development of microfluidic devices incorporating non-spherical hydrogel microparticles for protein-based bioassay

Dongkil Choi, Eunji Jang, Jin Won Park, Won-Gun Koh

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

This paper describes the fabrication of a microfluidic device for use in protein-based bioassays that effectively incorporates poly(ethylene glycol) (PEG) hydrogel microparticles within a defined region. The microfluidic device is composed of a polymerization chamber and reaction chamber that are serially connected through the microchannel. Various shapes and sizes of hydrogel microparticles were fabricated in the polymerization chamber by photopatterning and moved to the reaction chamber by pressure-driven flow. All of the hydrogel microparticles were retained within the reaction chamber due to an in-chamber integrated microfilter with smaller mesh size than hydrogel microparticles. Hydrogel microparticles were able to encapsulate enzymes without losing their activity, and different concentrations of glucose were detected by sequential bienzymatic reaction of hydrogel-entrapped glucose oxidase (GOX) and peroxidase (POD) inside the microfluidic device using fluorescence method. Importantly, there was a linear correspondence between fluorescence intensity and the glucose concentration over the physiologically important range of 1.00-10.00 mM.

Original languageEnglish
Pages (from-to)703-710
Number of pages8
JournalMicrofluidics and Nanofluidics
Volume5
Issue number5
DOIs
Publication statusPublished - 2008 May 27

Fingerprint

bioassay
Bioassay
microfluidic devices
Hydrogel
microparticles
Microfluidics
Hydrogels
chambers
proteins
Proteins
glucose
Polyethylene glycols
Glucose
polymerization
Fluorescence
Polymerization
fluorescence
Glucose Oxidase
Glucose oxidase
oxidase

All Science Journal Classification (ASJC) codes

  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials
  • Materials Chemistry

Cite this

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Development of microfluidic devices incorporating non-spherical hydrogel microparticles for protein-based bioassay. / Choi, Dongkil; Jang, Eunji; Park, Jin Won; Koh, Won-Gun.

In: Microfluidics and Nanofluidics, Vol. 5, No. 5, 27.05.2008, p. 703-710.

Research output: Contribution to journalArticle

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