Development of microfluidic devices incorporating non-spherical hydrogel microparticles for protein-based bioassay

Dongkil Choi, Eunji Jang, Jinwon Park, Won Gun Koh

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

This paper describes the fabrication of a microfluidic device for use in protein-based bioassays that effectively incorporates poly(ethylene glycol) (PEG) hydrogel microparticles within a defined region. The microfluidic device is composed of a polymerization chamber and reaction chamber that are serially connected through the microchannel. Various shapes and sizes of hydrogel microparticles were fabricated in the polymerization chamber by photopatterning and moved to the reaction chamber by pressure-driven flow. All of the hydrogel microparticles were retained within the reaction chamber due to an in-chamber integrated microfilter with smaller mesh size than hydrogel microparticles. Hydrogel microparticles were able to encapsulate enzymes without losing their activity, and different concentrations of glucose were detected by sequential bienzymatic reaction of hydrogel-entrapped glucose oxidase (GOX) and peroxidase (POD) inside the microfluidic device using fluorescence method. Importantly, there was a linear correspondence between fluorescence intensity and the glucose concentration over the physiologically important range of 1.00-10.00 mM.

Original languageEnglish
Pages (from-to)703-710
Number of pages8
JournalMicrofluidics and Nanofluidics
Volume5
Issue number5
DOIs
Publication statusPublished - 2008

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Materials Chemistry

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