Development of novel biocompatible thermosensitive anti-adhesive agents using human-derived acellular dermal matrix

Jong Ju Jeong, Dong Won Lee, Seung Yong Song, Yerin Park, Ji Hee Kim, Jang Il Kim, Hyung Goo Kim, Ki Taek Nam, Won Jai Lee, Kee Hyun Nam, Ju Hee Lee

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Abstract

Postoperative adhesion is a natural phenomenon that occurs in damaged tissue cells. Several anti-adhesion agents are currently used, but there is no leading-edge product with excellent adhesion-preventive effects. The purpose of this study was to develop ideal antiadhesive agents using human-derived acellular dermal matrix (ADM). We developed 5 new biocompatible thermosensitive anti-adhesion barriers (AABs) using micronized human-derived ADM, hyaluronic acid, and temperature-sensitive and biocompatible synthesized polymers. The biocompatibility, anti-adhesion effect, and biodegradability of these AABs were compared with those of commercial thermosensitive anti-adhesion agents. No cytotoxic effects were observed in vitro and in vivo. Animal testing of adhesion resistance confirmed that the adhesion area, strength, and grade of AAB03 were statistically superior to those of the control group. Factors related to adhesion formation, such as lymphocytes, macrophages, microvessels, and collagen fiber density, were observed using specific staining methods; the results confirmed that AAB03 group exhibited significantly lower macrophage counts, microvessel density, and collagen fiber density than the control groups. Furthermore, AAB03 was completely absorbed by 6 weeks. Thus, AAB03 has the potential to be used as a high-performance anti-adhesion agent.

Original languageEnglish
Article numbere0212583
JournalPloS one
Volume14
Issue number2
DOIs
Publication statusPublished - 2019 Feb

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All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Jeong, J. J., Lee, D. W., Song, S. Y., Park, Y., Kim, J. H., Kim, J. I., Kim, H. G., Nam, K. T., Lee, W. J., Nam, K. H., & Lee, J. H. (2019). Development of novel biocompatible thermosensitive anti-adhesive agents using human-derived acellular dermal matrix. PloS one, 14(2), [e0212583]. https://doi.org/10.1371/journal.pone.0212583