Development of phenol detecting biosensor using PEG hydrogel microparticles

Eunji Jang, Saemi Park, Hyun Jong Lee, Keshava Murthy, Won Gun Koh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this study, a simple and sensitive detection method for phenols were investigated using poly(ethylene glycol) (PEG) hydrogel microarray entrapping quantum dot (QD) and tyrosinase enzyme. Hydrogel microarrays were fabricated by using photo-patterning methods, and size of resultant hydrogel micropattern was ranged from 10 to 300 μm. The enzymatic reaction used for the detection of phenol is the oxidation of phenolic compounds via tyrosinase. In such reaction, phenol is oxidized to o-quinone which is a classic electron accepting chemical that can quench the fluorescence of QDs. Accuracy of the system was verified by measurement of photoluminescence (PL) quenching in free solution state with different concentrations of phenol. The PL quenching of QDs was investigated by varying the molecular weight of PEG, and concentration of QDs and/or enzymes.

Original languageEnglish
Title of host publicationINEC 2010 - 2010 3rd International Nanoelectronics Conference, Proceedings
Pages909-910
Number of pages2
DOIs
Publication statusPublished - 2010 May 5
Event2010 3rd International Nanoelectronics Conference, INEC 2010 - Hongkong, China
Duration: 2010 Jan 32010 Jan 8

Publication series

NameINEC 2010 - 2010 3rd International Nanoelectronics Conference, Proceedings

Other

Other2010 3rd International Nanoelectronics Conference, INEC 2010
CountryChina
CityHongkong
Period10/1/310/1/8

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

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    Jang, E., Park, S., Lee, H. J., Murthy, K., & Koh, W. G. (2010). Development of phenol detecting biosensor using PEG hydrogel microparticles. In INEC 2010 - 2010 3rd International Nanoelectronics Conference, Proceedings (pp. 909-910). [5425132] (INEC 2010 - 2010 3rd International Nanoelectronics Conference, Proceedings). https://doi.org/10.1109/INEC.2010.5425132