Development of phenol detecting biosensor using PEG hydrogel microparticles

Eunji Jang, Saemi Park, Hyun Jong Lee, Keshava Murthy, Won-Gun Koh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this study, a simple and sensitive detection method for phenols were investigated using poly(ethylene glycol) (PEG) hydrogel microarray entrapping quantum dot (QD) and tyrosinase enzyme. Hydrogel microarrays were fabricated by using photo-patterning methods, and size of resultant hydrogel micropattern was ranged from 10 to 300 μm. The enzymatic reaction used for the detection of phenol is the oxidation of phenolic compounds via tyrosinase. In such reaction, phenol is oxidized to o-quinone which is a classic electron accepting chemical that can quench the fluorescence of QDs. Accuracy of the system was verified by measurement of photoluminescence (PL) quenching in free solution state with different concentrations of phenol. The PL quenching of QDs was investigated by varying the molecular weight of PEG, and concentration of QDs and/or enzymes.

Original languageEnglish
Title of host publicationINEC 2010 - 2010 3rd International Nanoelectronics Conference, Proceedings
Pages909-910
Number of pages2
DOIs
Publication statusPublished - 2010 May 5
Event2010 3rd International Nanoelectronics Conference, INEC 2010 - Hongkong, China
Duration: 2010 Jan 32010 Jan 8

Other

Other2010 3rd International Nanoelectronics Conference, INEC 2010
CountryChina
CityHongkong
Period10/1/310/1/8

Fingerprint

Biosensors
Hydrogels
Polyethylene glycols
Phenols
Microarrays
Quenching
Photoluminescence
Enzymes
Semiconductor quantum dots
Fluorescence
Molecular weight
Oxidation
Electrons

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

Cite this

Jang, E., Park, S., Lee, H. J., Murthy, K., & Koh, W-G. (2010). Development of phenol detecting biosensor using PEG hydrogel microparticles. In INEC 2010 - 2010 3rd International Nanoelectronics Conference, Proceedings (pp. 909-910). [5425132] https://doi.org/10.1109/INEC.2010.5425132
Jang, Eunji ; Park, Saemi ; Lee, Hyun Jong ; Murthy, Keshava ; Koh, Won-Gun. / Development of phenol detecting biosensor using PEG hydrogel microparticles. INEC 2010 - 2010 3rd International Nanoelectronics Conference, Proceedings. 2010. pp. 909-910
@inproceedings{e6703ca3f6ba49d28f93dc0adc8f0b52,
title = "Development of phenol detecting biosensor using PEG hydrogel microparticles",
abstract = "In this study, a simple and sensitive detection method for phenols were investigated using poly(ethylene glycol) (PEG) hydrogel microarray entrapping quantum dot (QD) and tyrosinase enzyme. Hydrogel microarrays were fabricated by using photo-patterning methods, and size of resultant hydrogel micropattern was ranged from 10 to 300 μm. The enzymatic reaction used for the detection of phenol is the oxidation of phenolic compounds via tyrosinase. In such reaction, phenol is oxidized to o-quinone which is a classic electron accepting chemical that can quench the fluorescence of QDs. Accuracy of the system was verified by measurement of photoluminescence (PL) quenching in free solution state with different concentrations of phenol. The PL quenching of QDs was investigated by varying the molecular weight of PEG, and concentration of QDs and/or enzymes.",
author = "Eunji Jang and Saemi Park and Lee, {Hyun Jong} and Keshava Murthy and Won-Gun Koh",
year = "2010",
month = "5",
day = "5",
doi = "10.1109/INEC.2010.5425132",
language = "English",
isbn = "9781424435449",
pages = "909--910",
booktitle = "INEC 2010 - 2010 3rd International Nanoelectronics Conference, Proceedings",

}

Jang, E, Park, S, Lee, HJ, Murthy, K & Koh, W-G 2010, Development of phenol detecting biosensor using PEG hydrogel microparticles. in INEC 2010 - 2010 3rd International Nanoelectronics Conference, Proceedings., 5425132, pp. 909-910, 2010 3rd International Nanoelectronics Conference, INEC 2010, Hongkong, China, 10/1/3. https://doi.org/10.1109/INEC.2010.5425132

Development of phenol detecting biosensor using PEG hydrogel microparticles. / Jang, Eunji; Park, Saemi; Lee, Hyun Jong; Murthy, Keshava; Koh, Won-Gun.

INEC 2010 - 2010 3rd International Nanoelectronics Conference, Proceedings. 2010. p. 909-910 5425132.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Development of phenol detecting biosensor using PEG hydrogel microparticles

AU - Jang, Eunji

AU - Park, Saemi

AU - Lee, Hyun Jong

AU - Murthy, Keshava

AU - Koh, Won-Gun

PY - 2010/5/5

Y1 - 2010/5/5

N2 - In this study, a simple and sensitive detection method for phenols were investigated using poly(ethylene glycol) (PEG) hydrogel microarray entrapping quantum dot (QD) and tyrosinase enzyme. Hydrogel microarrays were fabricated by using photo-patterning methods, and size of resultant hydrogel micropattern was ranged from 10 to 300 μm. The enzymatic reaction used for the detection of phenol is the oxidation of phenolic compounds via tyrosinase. In such reaction, phenol is oxidized to o-quinone which is a classic electron accepting chemical that can quench the fluorescence of QDs. Accuracy of the system was verified by measurement of photoluminescence (PL) quenching in free solution state with different concentrations of phenol. The PL quenching of QDs was investigated by varying the molecular weight of PEG, and concentration of QDs and/or enzymes.

AB - In this study, a simple and sensitive detection method for phenols were investigated using poly(ethylene glycol) (PEG) hydrogel microarray entrapping quantum dot (QD) and tyrosinase enzyme. Hydrogel microarrays were fabricated by using photo-patterning methods, and size of resultant hydrogel micropattern was ranged from 10 to 300 μm. The enzymatic reaction used for the detection of phenol is the oxidation of phenolic compounds via tyrosinase. In such reaction, phenol is oxidized to o-quinone which is a classic electron accepting chemical that can quench the fluorescence of QDs. Accuracy of the system was verified by measurement of photoluminescence (PL) quenching in free solution state with different concentrations of phenol. The PL quenching of QDs was investigated by varying the molecular weight of PEG, and concentration of QDs and/or enzymes.

UR - http://www.scopus.com/inward/record.url?scp=77951664470&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77951664470&partnerID=8YFLogxK

U2 - 10.1109/INEC.2010.5425132

DO - 10.1109/INEC.2010.5425132

M3 - Conference contribution

SN - 9781424435449

SP - 909

EP - 910

BT - INEC 2010 - 2010 3rd International Nanoelectronics Conference, Proceedings

ER -

Jang E, Park S, Lee HJ, Murthy K, Koh W-G. Development of phenol detecting biosensor using PEG hydrogel microparticles. In INEC 2010 - 2010 3rd International Nanoelectronics Conference, Proceedings. 2010. p. 909-910. 5425132 https://doi.org/10.1109/INEC.2010.5425132