Development of 64Cu-loaded Perfluoropentane Nanodroplet: A Potential Tumor Theragnostic Nano-carrier and Dual-Modality PET-Ultrasound Imaging Agents

Unchol Shin, Jungyoung Kim, Jiwoong Lee, Donghee Park, Changmoon Lee, Hyon Chel Jung, Jiae Park, Kyochul Lee, Min Woo Lee, Suhng Wook Kim, Jongbum Seo

Research output: Contribution to journalArticlepeer-review

Abstract

The purpose of this study was to develop and preliminarily evaluate phospholipid-shelled nanodroplets (NDs) encapsulating perfluoropentane (PFP) and radioactive 64Cu as a hybrid positron emission tomography (PET)-ultrasound (US) probe. PFP NDs were fabricated by mixing liquid-phase PFP with a phospholipid solution. The 64Cu was encapsulated into the NDs in a size-controlled manner by exploiting the hydrophobicity of 64Cu-diacetyl-bis(N4-methylthiosemicarbazone) (64Cu-ATSM) using a vial mixer and an extruder. The fabricated 64Cu-loaded PFP NDs (64Cu-PFP NDs) were evaluated using in vitro/in vivo PET-computed tomography (PET-CT), US imaging and transmission electron microscopy. In the in vitro PET images, the 64Cu-PFP NDs were observed as a hot spot in the lower section of the test tube. In the acquired US images, the mean region of interest brightness values of 64Cu-PFP NDs were revealed by their strong echo image. In a tumor-bearing mouse animal model, tumor uptake of the 64Cu-PFP NDs was low, that is, approximately 65%, compared with that of only free 64Cu, as determined by PET-delayed imaging analysis. The dual-function concept of the NDs is expected to contribute to the prognosis and effectiveness of therapy by fusing the science and technology of nuclear medicine and US.

Original languageEnglish
Pages (from-to)2775-2784
Number of pages10
JournalUltrasound in Medicine and Biology
Volume46
Issue number10
DOIs
Publication statusPublished - 2020 Oct

Bibliographical note

Funding Information:
This work was supported by a Nuclear Research and Development Program of the National Research Foundation of Korea (NRF) grant funded by the Korean government (Nos. 2017 M2 A2 A6 A02019904 and 2020 R1 A2 C1008995). This work was also supported by a National Research Foundation of Korea (NRF) grant funded by the Ministry of Science, ICT & Future Planning (MSIP) (NRF-2014 M3 A6 A3063636) and a Korea University Grant.

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Radiological and Ultrasound Technology
  • Acoustics and Ultrasonics

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