Abstract
In this work we demonstrated the successful production of transgenic chickens expressing the enhanced green fluorescence protein (EGFP) gene. Replication-defective recombinant retroviruses produced from vesicular stomatitis virus G glycoprotein pseudotyped retrovirus vector system were injected beneath the blastoderm of non-incubated chicken embryos (stage X). From 129 injected eggs, 13 chicks hatched after 21 days of incubation. All hatched chicks were found to express vector-encoded EGFP gene, which was under the control of the Rous sarcoma virus promoter and boosted post-transcriptionally by woodchuck hepatitis virus post-transcriptional regulatory element sequence. Green fluorescent signals, indicative of the EGFP gene expression, were detected in various body parts, including head, limb, eye, toe, and several internal organs. Genomic incorporation of the transgene was also proven by Southern blot assay. Our results show the exceptional versatile effectiveness of the EGFP gene as a marker in the gene expression-related studies which therefore would be very helpful in establishing a useful transgenic chicken model system for studies on embryo development and for efficient production of transgenic chickens as bioreactors.
| Original language | English |
|---|---|
| Pages (from-to) | 442-448 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 320 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2004 Jul 23 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
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Development of transgenic chickens expressing enhanced green fluorescent protein. / Kwon, Mo Sun; Koo, Bon Chul; Choi, Bok Ruyl; Lee, Hoon Taek; Kim, Young Hye; Ryu, Wang-Shick; Shim, Hosup; Kim, Jin Hoi; Kim, Nam Hyung; Kim, Teoan.
In: Biochemical and Biophysical Research Communications, Vol. 320, No. 2, 23.07.2004, p. 442-448.Research output: Contribution to journal › Article
TY - JOUR
T1 - Development of transgenic chickens expressing enhanced green fluorescent protein
AU - Kwon, Mo Sun
AU - Koo, Bon Chul
AU - Choi, Bok Ruyl
AU - Lee, Hoon Taek
AU - Kim, Young Hye
AU - Ryu, Wang-Shick
AU - Shim, Hosup
AU - Kim, Jin Hoi
AU - Kim, Nam Hyung
AU - Kim, Teoan
PY - 2004/7/23
Y1 - 2004/7/23
N2 - In this work we demonstrated the successful production of transgenic chickens expressing the enhanced green fluorescence protein (EGFP) gene. Replication-defective recombinant retroviruses produced from vesicular stomatitis virus G glycoprotein pseudotyped retrovirus vector system were injected beneath the blastoderm of non-incubated chicken embryos (stage X). From 129 injected eggs, 13 chicks hatched after 21 days of incubation. All hatched chicks were found to express vector-encoded EGFP gene, which was under the control of the Rous sarcoma virus promoter and boosted post-transcriptionally by woodchuck hepatitis virus post-transcriptional regulatory element sequence. Green fluorescent signals, indicative of the EGFP gene expression, were detected in various body parts, including head, limb, eye, toe, and several internal organs. Genomic incorporation of the transgene was also proven by Southern blot assay. Our results show the exceptional versatile effectiveness of the EGFP gene as a marker in the gene expression-related studies which therefore would be very helpful in establishing a useful transgenic chicken model system for studies on embryo development and for efficient production of transgenic chickens as bioreactors.
AB - In this work we demonstrated the successful production of transgenic chickens expressing the enhanced green fluorescence protein (EGFP) gene. Replication-defective recombinant retroviruses produced from vesicular stomatitis virus G glycoprotein pseudotyped retrovirus vector system were injected beneath the blastoderm of non-incubated chicken embryos (stage X). From 129 injected eggs, 13 chicks hatched after 21 days of incubation. All hatched chicks were found to express vector-encoded EGFP gene, which was under the control of the Rous sarcoma virus promoter and boosted post-transcriptionally by woodchuck hepatitis virus post-transcriptional regulatory element sequence. Green fluorescent signals, indicative of the EGFP gene expression, were detected in various body parts, including head, limb, eye, toe, and several internal organs. Genomic incorporation of the transgene was also proven by Southern blot assay. Our results show the exceptional versatile effectiveness of the EGFP gene as a marker in the gene expression-related studies which therefore would be very helpful in establishing a useful transgenic chicken model system for studies on embryo development and for efficient production of transgenic chickens as bioreactors.
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UR - http://www.scopus.com/inward/citedby.url?scp=2942702007&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2004.05.197
DO - 10.1016/j.bbrc.2004.05.197
M3 - Article
VL - 320
SP - 442
EP - 448
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -