Development of transgenic chickens expressing enhanced green fluorescent protein

Mo Sun Kwon, Bon Chul Koo, Bok Ruyl Choi, Hoon Taek Lee, Young Hye Kim, Wang Shik Ryu, Hosup Shim, Jin Hoi Kim, Nam Hyung Kim, Teoan Kim

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35 Citations (Scopus)

Abstract

In this work we demonstrated the successful production of transgenic chickens expressing the enhanced green fluorescence protein (EGFP) gene. Replication-defective recombinant retroviruses produced from vesicular stomatitis virus G glycoprotein pseudotyped retrovirus vector system were injected beneath the blastoderm of non-incubated chicken embryos (stage X). From 129 injected eggs, 13 chicks hatched after 21 days of incubation. All hatched chicks were found to express vector-encoded EGFP gene, which was under the control of the Rous sarcoma virus promoter and boosted post-transcriptionally by woodchuck hepatitis virus post-transcriptional regulatory element sequence. Green fluorescent signals, indicative of the EGFP gene expression, were detected in various body parts, including head, limb, eye, toe, and several internal organs. Genomic incorporation of the transgene was also proven by Southern blot assay. Our results show the exceptional versatile effectiveness of the EGFP gene as a marker in the gene expression-related studies which therefore would be very helpful in establishing a useful transgenic chicken model system for studies on embryo development and for efficient production of transgenic chickens as bioreactors.

Original languageEnglish
Pages (from-to)442-448
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume320
Issue number2
DOIs
Publication statusPublished - 2004 Jul 23

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Kwon, M. S., Koo, B. C., Choi, B. R., Lee, H. T., Kim, Y. H., Ryu, W. S., Shim, H., Kim, J. H., Kim, N. H., & Kim, T. (2004). Development of transgenic chickens expressing enhanced green fluorescent protein. Biochemical and Biophysical Research Communications, 320(2), 442-448. https://doi.org/10.1016/j.bbrc.2004.05.197