Developmental stage- and DNA damage-specific functions of C. elegans FANCD2

Kyong Yun Lee; Insil Yang; Jung Eun Park; Ok Ryun Baek; Kee Yang Chung; Hyeon Sook Koo

doi:10.1016/j.bbrc.2006.11.039

Developmental stage- and DNA damage-specific functions of C. elegans FANCD2

Kyong Yun Lee, Insil Yang, Jung Eun Park, Ok Ryun Baek, Kee Yang Chung, Hyeon Sook Koo

Department of Biochemistry

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

In this study, we set out to investigate the role of Fanconi anemia complementation group D2 protein (FANCD2) in developmental stage-specific DNA damage responses in Caenorhabditis elegans. A mutant C. elegans strain containing a deletion in the gene encoding the FANCD2 homolog, FCD-2, exhibited egg-laying defects, precocious oogenesis, and partial defects in fertilization. The mutant strain also had a lower hatching rate than the wild-type after γ-irradiation of embryos, but not after the irradiation of pachytene stage germ cells. This mutation sensitized pachytene stage germ cells to the genotoxic effects of photoactivated psoralen, as seen by a greatly reduced hatching rate and increased chromosomal aberrations. This mutation also enhanced physiological M-phase arrest and apoptosis. Taken together, our data reveal that the C. elegans FANCD2 homolog participates in the repair of spontaneous DNA damage and DNA crosslinks, not only in proliferating cells but also in pachytene stage cells, and it may have an additional role in double-stranded DNA break repair during embryogenesis.

Original language	English
Pages (from-to)	479-485
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	352
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2006.11.039
Publication status	Published - 2007 Jan 12

Bibliographical note

Funding Information:
Caenorhabditis elegans N2 was obtained from the C. elegans Genetics Center (Minneapolis, MN, USA), which is supported by the National Center for Research Resources. The fcd-2 ( tm1298 ) strain was kindly provided by the National Bioresource Project (Japan). We thank Dr. Yuji Kohara (National Institute of Genetics, Japan) for fcd-2 EST clone yk293c3. This work was supported by Grant R01-2003-000-10396-0 from the Basic Research Program of the Korea Science and Engineering Foundation.

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/j.bbrc.2006.11.039

Cite this

@article{98a2e03ea4824f6ab04d98c9dc6a5758,

title = "Developmental stage- and DNA damage-specific functions of C. elegans FANCD2",

abstract = "In this study, we set out to investigate the role of Fanconi anemia complementation group D2 protein (FANCD2) in developmental stage-specific DNA damage responses in Caenorhabditis elegans. A mutant C. elegans strain containing a deletion in the gene encoding the FANCD2 homolog, FCD-2, exhibited egg-laying defects, precocious oogenesis, and partial defects in fertilization. The mutant strain also had a lower hatching rate than the wild-type after γ-irradiation of embryos, but not after the irradiation of pachytene stage germ cells. This mutation sensitized pachytene stage germ cells to the genotoxic effects of photoactivated psoralen, as seen by a greatly reduced hatching rate and increased chromosomal aberrations. This mutation also enhanced physiological M-phase arrest and apoptosis. Taken together, our data reveal that the C. elegans FANCD2 homolog participates in the repair of spontaneous DNA damage and DNA crosslinks, not only in proliferating cells but also in pachytene stage cells, and it may have an additional role in double-stranded DNA break repair during embryogenesis.",

author = "Lee, {Kyong Yun} and Insil Yang and Park, {Jung Eun} and Baek, {Ok Ryun} and Chung, {Kee Yang} and Koo, {Hyeon Sook}",

note = "Funding Information: Caenorhabditis elegans N2 was obtained from the C. elegans Genetics Center (Minneapolis, MN, USA), which is supported by the National Center for Research Resources. The fcd-2 ( tm1298 ) strain was kindly provided by the National Bioresource Project (Japan). We thank Dr. Yuji Kohara (National Institute of Genetics, Japan) for fcd-2 EST clone yk293c3. This work was supported by Grant R01-2003-000-10396-0 from the Basic Research Program of the Korea Science and Engineering Foundation. ",

year = "2007",

month = jan,

day = "12",

doi = "10.1016/j.bbrc.2006.11.039",

language = "English",

volume = "352",

pages = "479--485",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Developmental stage- and DNA damage-specific functions of C. elegans FANCD2

AU - Lee, Kyong Yun

AU - Yang, Insil

AU - Park, Jung Eun

AU - Baek, Ok Ryun

AU - Chung, Kee Yang

AU - Koo, Hyeon Sook

N1 - Funding Information: Caenorhabditis elegans N2 was obtained from the C. elegans Genetics Center (Minneapolis, MN, USA), which is supported by the National Center for Research Resources. The fcd-2 ( tm1298 ) strain was kindly provided by the National Bioresource Project (Japan). We thank Dr. Yuji Kohara (National Institute of Genetics, Japan) for fcd-2 EST clone yk293c3. This work was supported by Grant R01-2003-000-10396-0 from the Basic Research Program of the Korea Science and Engineering Foundation.

PY - 2007/1/12

Y1 - 2007/1/12

N2 - In this study, we set out to investigate the role of Fanconi anemia complementation group D2 protein (FANCD2) in developmental stage-specific DNA damage responses in Caenorhabditis elegans. A mutant C. elegans strain containing a deletion in the gene encoding the FANCD2 homolog, FCD-2, exhibited egg-laying defects, precocious oogenesis, and partial defects in fertilization. The mutant strain also had a lower hatching rate than the wild-type after γ-irradiation of embryos, but not after the irradiation of pachytene stage germ cells. This mutation sensitized pachytene stage germ cells to the genotoxic effects of photoactivated psoralen, as seen by a greatly reduced hatching rate and increased chromosomal aberrations. This mutation also enhanced physiological M-phase arrest and apoptosis. Taken together, our data reveal that the C. elegans FANCD2 homolog participates in the repair of spontaneous DNA damage and DNA crosslinks, not only in proliferating cells but also in pachytene stage cells, and it may have an additional role in double-stranded DNA break repair during embryogenesis.

AB - In this study, we set out to investigate the role of Fanconi anemia complementation group D2 protein (FANCD2) in developmental stage-specific DNA damage responses in Caenorhabditis elegans. A mutant C. elegans strain containing a deletion in the gene encoding the FANCD2 homolog, FCD-2, exhibited egg-laying defects, precocious oogenesis, and partial defects in fertilization. The mutant strain also had a lower hatching rate than the wild-type after γ-irradiation of embryos, but not after the irradiation of pachytene stage germ cells. This mutation sensitized pachytene stage germ cells to the genotoxic effects of photoactivated psoralen, as seen by a greatly reduced hatching rate and increased chromosomal aberrations. This mutation also enhanced physiological M-phase arrest and apoptosis. Taken together, our data reveal that the C. elegans FANCD2 homolog participates in the repair of spontaneous DNA damage and DNA crosslinks, not only in proliferating cells but also in pachytene stage cells, and it may have an additional role in double-stranded DNA break repair during embryogenesis.

UR - http://www.scopus.com/inward/record.url?scp=33751506586&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33751506586&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2006.11.039

DO - 10.1016/j.bbrc.2006.11.039

M3 - Article

C2 - 17126808

AN - SCOPUS:33751506586

VL - 352

SP - 479

EP - 485

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -

Developmental stage- and DNA damage-specific functions of C. elegans FANCD2

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this