Developmental stage- and DNA damage-specific functions of C. elegans FANCD2

Kyong Yun Lee, Insil Yang, Jung Eun Park, Ok Ryun Baek, Kee Yang Chung, Hyeon Sook Koo

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In this study, we set out to investigate the role of Fanconi anemia complementation group D2 protein (FANCD2) in developmental stage-specific DNA damage responses in Caenorhabditis elegans. A mutant C. elegans strain containing a deletion in the gene encoding the FANCD2 homolog, FCD-2, exhibited egg-laying defects, precocious oogenesis, and partial defects in fertilization. The mutant strain also had a lower hatching rate than the wild-type after γ-irradiation of embryos, but not after the irradiation of pachytene stage germ cells. This mutation sensitized pachytene stage germ cells to the genotoxic effects of photoactivated psoralen, as seen by a greatly reduced hatching rate and increased chromosomal aberrations. This mutation also enhanced physiological M-phase arrest and apoptosis. Taken together, our data reveal that the C. elegans FANCD2 homolog participates in the repair of spontaneous DNA damage and DNA crosslinks, not only in proliferating cells but also in pachytene stage cells, and it may have an additional role in double-stranded DNA break repair during embryogenesis.

Original languageEnglish
Pages (from-to)479-485
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume352
Issue number2
DOIs
Publication statusPublished - 2007 Jan 12

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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