Diagnosis of severe sepsis using phospholipids enzymatic assay based on cyclic voltammetry

Jun Hee Park, Jong Min Park, Ga Yeon Lee, Zhiquan Song, Jeong Soo Sung, Min Jung Kang, Nam Su Ku, Eun Hye Lee, Kwang Hwan Park, Moo Suk Park, Sang Guk Lee, Jae Chul Pyun

Research output: Contribution to journalArticlepeer-review

Abstract

In this work phospholipid quantification was carried out using an enzymatic assay based on cyclic voltammetry of the condensation product of N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline sodium salt (DAOS) and 4-aminoantipyrine (4-AP) with a graphite electrode. For the optimization of electrochemical measurement for the product, electrochemical properties such as the electrochemical window, double layer capacitance (Cdl) and electron transfer rate (kapp) were analyzed for a graphite-electrode and Au-electrode. The phospholipid enzymatic assay based the on electrochemical measurement using the graphite electrode was applied to the diagnosis of sepsis for sera from healthy volunteers (n = 16), patients with systemic inflammatory response syndrome (SIRS, n = 16) and severe sepsis patients (n = 24). Finally, the phospholipid quantification results from the electrochemical measurement were statistically compared with the conventional method based on optical density measurement.

Original languageEnglish
Article number109728
JournalEnzyme and Microbial Technology
Volume144
DOIs
Publication statusPublished - 2021 Mar

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea [grant number: NRF-2020R1A2B5B01002187 and NRF-2020R1A5A101913111 ].

Publisher Copyright:
© 2020 Elsevier Inc.

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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