Diamond-like carbon (DLC) microelectrode for electrochemical ELISA

Jo Il Kim, Adela Bordeanu, Jae Chul Pyun

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

A diamond-like carbon (DLC) microelectrode was applied to commercial ELISA kits for medical diagnosis of HIV (human immunodeficiency virus), HBV (human hepatitis B virus), HCV (human hepatitis C virus). In this work, quantification of oxidized 3,3′,5,5′-tetramethylbenzidine (TMB) was carried out by using a microelectrode made of boron-doped DLC and cyclic voltammetric analysis method without the conventional quenching step which uses sulfuric acid. The microelectrode provided well-known step-shaped graphs, and limit of detection could be improved by clear determination of electrochemical oxidative and reductive peaks. To demonstrate the applicability of DLC microelectrode to conventional ELISA kits, commercial ELISA kits for detection of HIV antigen, HBV antigen and HCV antigen were also tested. These results proved that the applicability of DLC microelectrode to practical detection is feasible.

Original languageEnglish
Pages (from-to)1394-1398
Number of pages5
JournalBiosensors and Bioelectronics
Volume24
Issue number5
DOIs
Publication statusPublished - 2009 Jan 1

Fingerprint

Diamond
Microelectrodes
Viruses
Diamonds
Carbon
Enzyme-Linked Immunosorbent Assay
Antigens
Hepatitis B virus
Hepacivirus
Hepatitis C Antigens
HIV
Hepatitis B Antigens
Boron
Fourier Analysis
Limit of Detection
Sulfuric acid
Quenching

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biomedical Engineering
  • Biotechnology
  • Electrochemistry

Cite this

Kim, Jo Il ; Bordeanu, Adela ; Pyun, Jae Chul. / Diamond-like carbon (DLC) microelectrode for electrochemical ELISA. In: Biosensors and Bioelectronics. 2009 ; Vol. 24, No. 5. pp. 1394-1398.
@article{fe3349cc87134d108487384dfa4eeca4,
title = "Diamond-like carbon (DLC) microelectrode for electrochemical ELISA",
abstract = "A diamond-like carbon (DLC) microelectrode was applied to commercial ELISA kits for medical diagnosis of HIV (human immunodeficiency virus), HBV (human hepatitis B virus), HCV (human hepatitis C virus). In this work, quantification of oxidized 3,3′,5,5′-tetramethylbenzidine (TMB) was carried out by using a microelectrode made of boron-doped DLC and cyclic voltammetric analysis method without the conventional quenching step which uses sulfuric acid. The microelectrode provided well-known step-shaped graphs, and limit of detection could be improved by clear determination of electrochemical oxidative and reductive peaks. To demonstrate the applicability of DLC microelectrode to conventional ELISA kits, commercial ELISA kits for detection of HIV antigen, HBV antigen and HCV antigen were also tested. These results proved that the applicability of DLC microelectrode to practical detection is feasible.",
author = "Kim, {Jo Il} and Adela Bordeanu and Pyun, {Jae Chul}",
year = "2009",
month = "1",
day = "1",
doi = "10.1016/j.bios.2008.08.003",
language = "English",
volume = "24",
pages = "1394--1398",
journal = "Biosensors and Bioelectronics",
issn = "0956-5663",
publisher = "Elsevier Limited",
number = "5",

}

Diamond-like carbon (DLC) microelectrode for electrochemical ELISA. / Kim, Jo Il; Bordeanu, Adela; Pyun, Jae Chul.

In: Biosensors and Bioelectronics, Vol. 24, No. 5, 01.01.2009, p. 1394-1398.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Diamond-like carbon (DLC) microelectrode for electrochemical ELISA

AU - Kim, Jo Il

AU - Bordeanu, Adela

AU - Pyun, Jae Chul

PY - 2009/1/1

Y1 - 2009/1/1

N2 - A diamond-like carbon (DLC) microelectrode was applied to commercial ELISA kits for medical diagnosis of HIV (human immunodeficiency virus), HBV (human hepatitis B virus), HCV (human hepatitis C virus). In this work, quantification of oxidized 3,3′,5,5′-tetramethylbenzidine (TMB) was carried out by using a microelectrode made of boron-doped DLC and cyclic voltammetric analysis method without the conventional quenching step which uses sulfuric acid. The microelectrode provided well-known step-shaped graphs, and limit of detection could be improved by clear determination of electrochemical oxidative and reductive peaks. To demonstrate the applicability of DLC microelectrode to conventional ELISA kits, commercial ELISA kits for detection of HIV antigen, HBV antigen and HCV antigen were also tested. These results proved that the applicability of DLC microelectrode to practical detection is feasible.

AB - A diamond-like carbon (DLC) microelectrode was applied to commercial ELISA kits for medical diagnosis of HIV (human immunodeficiency virus), HBV (human hepatitis B virus), HCV (human hepatitis C virus). In this work, quantification of oxidized 3,3′,5,5′-tetramethylbenzidine (TMB) was carried out by using a microelectrode made of boron-doped DLC and cyclic voltammetric analysis method without the conventional quenching step which uses sulfuric acid. The microelectrode provided well-known step-shaped graphs, and limit of detection could be improved by clear determination of electrochemical oxidative and reductive peaks. To demonstrate the applicability of DLC microelectrode to conventional ELISA kits, commercial ELISA kits for detection of HIV antigen, HBV antigen and HCV antigen were also tested. These results proved that the applicability of DLC microelectrode to practical detection is feasible.

UR - http://www.scopus.com/inward/record.url?scp=58149156560&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=58149156560&partnerID=8YFLogxK

U2 - 10.1016/j.bios.2008.08.003

DO - 10.1016/j.bios.2008.08.003

M3 - Article

VL - 24

SP - 1394

EP - 1398

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

SN - 0956-5663

IS - 5

ER -