DIC-1 over-expression enhances respiratory activity in Caenorhabditis elegans by promoting mitochondrial cristae formation

Tae Hoon Lee, Ji Young Mun, Sung Min Han, Gyesoon Yoon, Sung Sik Han, Hyeon Sook Koo

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Deficiency of the Caenorhabditis elegans protein, DIC-1, located in the inner membrane of mitochondria produces an abnormal mitochondrial morphology. The mechanism by which DIC-1 controls the topology of the inner membrane was investigated by transiently over-expressing DIC-1 in C. elegans. Cryo-electron microscopy showed that DIC-1 over-expression greatly increased the number and fractional area of mitochondrial cristae, suggesting that DIC-1 actively participates in cristae formation. These morphological changes were accompanied by increases in the oxygen consumption rate and ATP content of C. elegans worms, and decreases in reactive oxygen species (ROS) and sensitivity to paraquat. DIC-1 knockdown induced the opposite changes in ATP, ROS and paraquat-sensitivity. The ability of DIC-1 to increase cristae formation and secondarily, oxidative phosphorylation, suggests a potential use of this factor to control mitochondrial activity.

Original languageEnglish
Pages (from-to)319-327
Number of pages9
JournalGenes to Cells
Volume14
Issue number3
DOIs
Publication statusPublished - 2009 Mar 6

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

Cite this