Diet-induced obesity regulates the galanin-mediated signaling cascade in the adipose tissue of mice

Ahreum Kim, Taesun Park

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Galanin is a neuropeptide that regulates the food intake, neurogenesis, memory, and gut secretion. This study was conducted to evaluate the high-fat diet (HFD)-induced regulation of the galanin receptors (GalRs) and the associated signaling molecules in the adipose tissues of mice. Twenty C57BL/6J mice were given either an HFD or a normal diet for 12 wk. The results of the semiquantitative RT-PCR analyses indicated that the HFD upregulated the expression of GalR1, GalR2, GalR3, resistance to audiogenic seizures, peroxisome proliferator-activated receptorγ2, adipocyte protein 2, and protein kinase Cδ and downregulated the expression of peroxisome proliferative activated receptor γ coactivator 1α and uncoupling protein 1 in the adipose tissues. The immunoblot results showed that the protein levels of peroxisome proliferator-activated receptorγ 2 and adipocyte protein 2, and the phosphorylation of c-Raf and extracellular signal-regulated kinase 1/2 were increased, while the phosphorylation of cyclic adenosine monophosphate-responsive element-binding protein, which regulates peroxisome proliferative activated receptor γ coactivator 1α and uncoupling protein 1, was decreased in the epididymal adipose tissues of the HFD-fed mice. These results suggest the possible association of the galanin-mediated signaling pathways in the manifestation of the HFD-induced activation of adipogenesis along with the suppression of thermogenesis in the adipose tissues of mice.

Original languageEnglish
Pages (from-to)1361-1370
Number of pages10
JournalMolecular Nutrition and Food Research
Volume54
Issue number9
DOIs
Publication statusPublished - 2010 Sep 1

Fingerprint

Galanin
High Fat Diet
high fat diet
adipose tissue
Adipose Tissue
obesity
Obesity
Diet
peroxisomes
mice
diet
Peroxisomes
adipocytes
Adipocytes
proteins
receptors
phosphorylation
Galanin Receptors
Phosphorylation
Peroxisome Proliferators

All Science Journal Classification (ASJC) codes

  • Food Science
  • Biotechnology

Cite this

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abstract = "Galanin is a neuropeptide that regulates the food intake, neurogenesis, memory, and gut secretion. This study was conducted to evaluate the high-fat diet (HFD)-induced regulation of the galanin receptors (GalRs) and the associated signaling molecules in the adipose tissues of mice. Twenty C57BL/6J mice were given either an HFD or a normal diet for 12 wk. The results of the semiquantitative RT-PCR analyses indicated that the HFD upregulated the expression of GalR1, GalR2, GalR3, resistance to audiogenic seizures, peroxisome proliferator-activated receptorγ2, adipocyte protein 2, and protein kinase Cδ and downregulated the expression of peroxisome proliferative activated receptor γ coactivator 1α and uncoupling protein 1 in the adipose tissues. The immunoblot results showed that the protein levels of peroxisome proliferator-activated receptorγ 2 and adipocyte protein 2, and the phosphorylation of c-Raf and extracellular signal-regulated kinase 1/2 were increased, while the phosphorylation of cyclic adenosine monophosphate-responsive element-binding protein, which regulates peroxisome proliferative activated receptor γ coactivator 1α and uncoupling protein 1, was decreased in the epididymal adipose tissues of the HFD-fed mice. These results suggest the possible association of the galanin-mediated signaling pathways in the manifestation of the HFD-induced activation of adipogenesis along with the suppression of thermogenesis in the adipose tissues of mice.",
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Diet-induced obesity regulates the galanin-mediated signaling cascade in the adipose tissue of mice. / Kim, Ahreum; Park, Taesun.

In: Molecular Nutrition and Food Research, Vol. 54, No. 9, 01.09.2010, p. 1361-1370.

Research output: Contribution to journalArticle

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