Dietary monounsaturated fatty acids but not saturated fatty acids preserve the insulin signaling pathway via IRS-1/PI3K in rat skeletal muscle

Jae Hoon Moon, Ji Young Lee, Saet Byol Kang, Jong Suk Park, byungwan lee, Eun Seok Kang, Chul Woo Ahn, Hyun Chul Lee, Bong Soo Cha

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Saturated fatty acids (SFA) and monounsaturated fatty acids (MUFA) show different effects on the development of insulin resistance. In this study, we compared the effect of dietary SFA and MUFA on the insulin signaling pathway in the skeletal muscle of a type 2 diabetic animal model. Twenty-nine-week-old male Otsuka Long-Evans Tokushima fatty (OLETF) rats were randomly divided into three groups and fed one of the following diets for 3 weeks; a normal chow diet, an SFA (lard oil) enriched or a MUFA (olive oil) enriched high-fat diet. The vastus lateralis muscle was used for analyses. Insulin tolerance test showed improved insulin sensitivity in rats fed the MUFA diet, as compared to those fed the SFA diet (p <0.001). The SFA diet reduced IRS-1 expression and phosphorylated PI3K levels in skeletal muscle, as compared with a chow diet (p <0.001, respectively). On the contrary, muscle IRS-2 expression and phosphorylated ERK1/2 was significantly increased in rats fed the SFA diet (p <0.001, respectively). Membrane translocation of glucose transporter type 4 decreased in the skeletal muscle of rats fed the SFA diet, as compared to those fed a chow diet (p <0.001). These changes in insulin signaling pathway in skeletal muscle were not observed in rats fed the MUFA diet. In conclusion, the beneficial effect of dietary MUFA on insulin sensitivity is associated with a conserved IRS-1/PI3K insulin signaling pathway which was altered by dietary SFA.

Original languageEnglish
Pages (from-to)1109-1116
Number of pages8
JournalLipids
Volume45
Issue number12
DOIs
Publication statusPublished - 2010 Dec 1

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Monounsaturated Fatty Acids
Nutrition
Phosphatidylinositol 3-Kinases
Muscle
Rats
Skeletal Muscle
Fatty Acids
Insulin
Diet
Insulin Resistance
Inbred OLETF Rats
Glucose Transporter Type 4
Muscles
Quadriceps Muscle
High Fat Diet
Oils
Animal Models
Animals
Fats

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Organic Chemistry
  • Cell Biology

Cite this

Moon, Jae Hoon ; Lee, Ji Young ; Kang, Saet Byol ; Park, Jong Suk ; lee, byungwan ; Kang, Eun Seok ; Ahn, Chul Woo ; Lee, Hyun Chul ; Cha, Bong Soo. / Dietary monounsaturated fatty acids but not saturated fatty acids preserve the insulin signaling pathway via IRS-1/PI3K in rat skeletal muscle. In: Lipids. 2010 ; Vol. 45, No. 12. pp. 1109-1116.
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abstract = "Saturated fatty acids (SFA) and monounsaturated fatty acids (MUFA) show different effects on the development of insulin resistance. In this study, we compared the effect of dietary SFA and MUFA on the insulin signaling pathway in the skeletal muscle of a type 2 diabetic animal model. Twenty-nine-week-old male Otsuka Long-Evans Tokushima fatty (OLETF) rats were randomly divided into three groups and fed one of the following diets for 3 weeks; a normal chow diet, an SFA (lard oil) enriched or a MUFA (olive oil) enriched high-fat diet. The vastus lateralis muscle was used for analyses. Insulin tolerance test showed improved insulin sensitivity in rats fed the MUFA diet, as compared to those fed the SFA diet (p <0.001). The SFA diet reduced IRS-1 expression and phosphorylated PI3K levels in skeletal muscle, as compared with a chow diet (p <0.001, respectively). On the contrary, muscle IRS-2 expression and phosphorylated ERK1/2 was significantly increased in rats fed the SFA diet (p <0.001, respectively). Membrane translocation of glucose transporter type 4 decreased in the skeletal muscle of rats fed the SFA diet, as compared to those fed a chow diet (p <0.001). These changes in insulin signaling pathway in skeletal muscle were not observed in rats fed the MUFA diet. In conclusion, the beneficial effect of dietary MUFA on insulin sensitivity is associated with a conserved IRS-1/PI3K insulin signaling pathway which was altered by dietary SFA.",
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Dietary monounsaturated fatty acids but not saturated fatty acids preserve the insulin signaling pathway via IRS-1/PI3K in rat skeletal muscle. / Moon, Jae Hoon; Lee, Ji Young; Kang, Saet Byol; Park, Jong Suk; lee, byungwan; Kang, Eun Seok; Ahn, Chul Woo; Lee, Hyun Chul; Cha, Bong Soo.

In: Lipids, Vol. 45, No. 12, 01.12.2010, p. 1109-1116.

Research output: Contribution to journalArticle

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