Difference of interferon-α and interferon-β on melanoma growth and lymph node metastasis in mice

MiRyung Roh, Zhenlong Zheng, Hyun Sook Kim, Hei Cheul Jeung, SunYoung Rha, Kee Yang Chung

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Interferon (IFN)-α and IFN-β are type I IFNs which are known to exert an antitumor effect on malignant melanoma. The aim of this study was to evaluate and compare the efficacy of IFN-α2b and IFN-β1a on primary tumor growth and lymph node metastasis, and to examine the mechanisms of lymph node metastasis. The efficacy of IFN-α2b and IFN-β1a was evaluated using a human melanoma xenograft model. We further examined the effect of IFNs on lymphangiogenic growth factors in human melanoma cells. IFN-β1a showed a stronger antiproliferative and proapoptotic effect, whereas IFN-α2b inhibited tumor growth and lymph node metastasis through inhibition of lymphangiogenesis. Both IFN-α2b and IFN-β1a were effective in inhibiting lymph node metastasis compared with the control. Microvessel density decreased in tumors treated with IFN-α2b and IFN-β1a compared with the control, without statistical significance. Lymphatic vessel density decreased significantly only in tumors treated with IFN-α2b (P<0.05). Both IFN-α2b and IFN-β1a decreased in-vitro and in-vivo vascular endothelial growth factor (VEGF)-C and VEGF receptor-3 protein expression and secretory VEGF-C level in vitro. IFN-α2b showed an earlier and sustained effect in decreasing VEGF-C and VEGF receptor-3 protein expression and a superior effect in decreasing the secretory VEGF-C level compared with IFN-β1a. Our investigation shows that both IFN-α2b and IFN-β1a exerted different antitumor and antimetastatic effects in human melanoma xenograft. Moreover, the present findings indicate that inhibition of lymphangiogenesis is another possible antimetastatic action mechanism of IFN-α2b.

Original languageEnglish
Pages (from-to)114-124
Number of pages11
JournalMelanoma Research
Volume23
Issue number2
DOIs
Publication statusPublished - 2013 Apr 1

Fingerprint

Interferons
Melanoma
Lymph Nodes
Neoplasm Metastasis
Growth
Vascular Endothelial Growth Factor C
Vascular Endothelial Growth Factor Receptor-3
Lymphangiogenesis
Heterografts
Neoplasms
Interferon Type I
Lymphatic Vessels
Microvessels

All Science Journal Classification (ASJC) codes

  • Oncology
  • Dermatology
  • Cancer Research

Cite this

Roh, MiRyung ; Zheng, Zhenlong ; Kim, Hyun Sook ; Jeung, Hei Cheul ; Rha, SunYoung ; Chung, Kee Yang. / Difference of interferon-α and interferon-β on melanoma growth and lymph node metastasis in mice. In: Melanoma Research. 2013 ; Vol. 23, No. 2. pp. 114-124.
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abstract = "Interferon (IFN)-α and IFN-β are type I IFNs which are known to exert an antitumor effect on malignant melanoma. The aim of this study was to evaluate and compare the efficacy of IFN-α2b and IFN-β1a on primary tumor growth and lymph node metastasis, and to examine the mechanisms of lymph node metastasis. The efficacy of IFN-α2b and IFN-β1a was evaluated using a human melanoma xenograft model. We further examined the effect of IFNs on lymphangiogenic growth factors in human melanoma cells. IFN-β1a showed a stronger antiproliferative and proapoptotic effect, whereas IFN-α2b inhibited tumor growth and lymph node metastasis through inhibition of lymphangiogenesis. Both IFN-α2b and IFN-β1a were effective in inhibiting lymph node metastasis compared with the control. Microvessel density decreased in tumors treated with IFN-α2b and IFN-β1a compared with the control, without statistical significance. Lymphatic vessel density decreased significantly only in tumors treated with IFN-α2b (P<0.05). Both IFN-α2b and IFN-β1a decreased in-vitro and in-vivo vascular endothelial growth factor (VEGF)-C and VEGF receptor-3 protein expression and secretory VEGF-C level in vitro. IFN-α2b showed an earlier and sustained effect in decreasing VEGF-C and VEGF receptor-3 protein expression and a superior effect in decreasing the secretory VEGF-C level compared with IFN-β1a. Our investigation shows that both IFN-α2b and IFN-β1a exerted different antitumor and antimetastatic effects in human melanoma xenograft. Moreover, the present findings indicate that inhibition of lymphangiogenesis is another possible antimetastatic action mechanism of IFN-α2b.",
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Difference of interferon-α and interferon-β on melanoma growth and lymph node metastasis in mice. / Roh, MiRyung; Zheng, Zhenlong; Kim, Hyun Sook; Jeung, Hei Cheul; Rha, SunYoung; Chung, Kee Yang.

In: Melanoma Research, Vol. 23, No. 2, 01.04.2013, p. 114-124.

Research output: Contribution to journalArticle

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