Different effects of resveratrol on early and late passage mesenchymal stem cells through β-catenin regulation

Dong Suk Yoon, Yoorim Choi, Seong Mi Choi, Kwang Hwan Park, jinwoo lee

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Resveratrol is a sirtuin 1 (SIRT1) activator and can function as an anti-inflammatory and antioxidant factor. In mesenchymal stem cells (MSCs), resveratrol enhances the proliferation and differentiation potential and has an anti-aging effect. However, contradictory effects of resveratrol on MSC cultures have been reported. In this study, we found that resveratrol had different effects on MSC cultures according to their cell passage and SIRT1 expression. Resveratrol enhanced the self-renewal potential and multipotency of early passage MSCs, but accelerated cellular senescence of late passage MSCs. In early passage MSCs expressing SIRT1, resveratrol decreased ERK and GSK-3β phosphorylation, suppressing β-catenin activity. In contrast, in late passage MSCs, which did not express SIRT1, resveratrol increased ERK and GSK-3β phosphorylation, activating β-catenin. We confirmed that SIRT1-deficient early passage MSCs treated with resveratrol lost their self-renewal potential and multipotency, and became senescent due to increased β-catenin activity. Sustained treatment with resveratrol at early passages maintained the self-renewal potential and multipotency of MSCs up to passage 10. Our findings suggest that resveratrol can be effectively applied to early passage MSC cultures, whereas parameters such as cell passage and SIRT1 expression must be taken into consideration before applying resveratrol to late passage MSCs.

Original languageEnglish
Pages (from-to)1026-1032
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume467
Issue number4
DOIs
Publication statusPublished - 2015 Nov 27

Fingerprint

Catenins
Stem cells
Mesenchymal Stromal Cells
Sirtuin 1
Cell culture
Glycogen Synthase Kinase 3
Phosphorylation
Cell Culture Techniques
resveratrol
Activator Appliances
Cell Aging
Anti-Inflammatory Agents
Antioxidants
Aging of materials

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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abstract = "Resveratrol is a sirtuin 1 (SIRT1) activator and can function as an anti-inflammatory and antioxidant factor. In mesenchymal stem cells (MSCs), resveratrol enhances the proliferation and differentiation potential and has an anti-aging effect. However, contradictory effects of resveratrol on MSC cultures have been reported. In this study, we found that resveratrol had different effects on MSC cultures according to their cell passage and SIRT1 expression. Resveratrol enhanced the self-renewal potential and multipotency of early passage MSCs, but accelerated cellular senescence of late passage MSCs. In early passage MSCs expressing SIRT1, resveratrol decreased ERK and GSK-3β phosphorylation, suppressing β-catenin activity. In contrast, in late passage MSCs, which did not express SIRT1, resveratrol increased ERK and GSK-3β phosphorylation, activating β-catenin. We confirmed that SIRT1-deficient early passage MSCs treated with resveratrol lost their self-renewal potential and multipotency, and became senescent due to increased β-catenin activity. Sustained treatment with resveratrol at early passages maintained the self-renewal potential and multipotency of MSCs up to passage 10. Our findings suggest that resveratrol can be effectively applied to early passage MSC cultures, whereas parameters such as cell passage and SIRT1 expression must be taken into consideration before applying resveratrol to late passage MSCs.",
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Different effects of resveratrol on early and late passage mesenchymal stem cells through β-catenin regulation. / Yoon, Dong Suk; Choi, Yoorim; Choi, Seong Mi; Park, Kwang Hwan; lee, jinwoo.

In: Biochemical and Biophysical Research Communications, Vol. 467, No. 4, 27.11.2015, p. 1026-1032.

Research output: Contribution to journalArticle

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AU - Choi, Yoorim

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