Differential control of interleukin-6 mRNA levels by cellular distribution of YB-1

Sujin Kang, Taeyun A. Lee, Eun A. Ra, Eunhye Lee, Hyun Jin Choi, Sungwook Lee, Boyoun Park

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Cytokine production is essential for innate and adaptive immunity against microbial invaders and must be tightly controlled. Cytokine messenger RNA (mRNA) is in constant flux between the nucleus and the cytoplasm and in transcription, splicing, or decay; such processes must be tightly controlled. Here, we report a novel function of Y-box-binding protein 1 (YB-1) in modulating interleukin-6 (IL-6) mRNA levels in a cell type-specific manner. In lipopolysaccharide (LPS)-stimulated macrophages, YB-1 interacts with IL-6 mRNA and actively transports it to the extracellular space by YB-1-enriched vesicles, resulting in the proper maintenance of intracellular IL-6 mRNA levels. YB-1 secretion occurs in a cell type-specific manner. Whereas macrophages actively secret YB-1, dendritic cells maintain it predominantly in the cytoplasm even in response to LPS. Intracellular YB-1 has the distinct function of regulating IL-6 mRNA stability in dendritic cells. Moreover, because LPS differentially regulates the expression of histone deacetylase 6 (HDAC6) in macrophages and dendritic cells, this stimulus might control YB-1 acetylation differentially in both cell types. Taken together, these results suggest a unique feature of YB-1 in controlling intracellular IL-6 mRNA levels in a cell type-specific manner, thereby leading to functions that are dependent on the extracellular and intracellular distribution of YB-1.

Original languageEnglish
Article numbere112754
JournalPloS one
Volume9
Issue number11
DOIs
Publication statusPublished - 2014 Nov 14

Fingerprint

messenger RNA
interleukin-6
Interleukin-6
Messenger RNA
Macrophages
dendritic cells
Dendritic Cells
lipopolysaccharides
Lipopolysaccharides
macrophages
Y-Box-Binding Protein 1
Cytoplasm
cytokines
cytoplasm
RNA Transport
cells
Cytokines
Acetylation
histone deacetylase
Histone Deacetylases

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Kang, Sujin ; Lee, Taeyun A. ; Ra, Eun A. ; Lee, Eunhye ; Choi, Hyun Jin ; Lee, Sungwook ; Park, Boyoun. / Differential control of interleukin-6 mRNA levels by cellular distribution of YB-1. In: PloS one. 2014 ; Vol. 9, No. 11.
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Differential control of interleukin-6 mRNA levels by cellular distribution of YB-1. / Kang, Sujin; Lee, Taeyun A.; Ra, Eun A.; Lee, Eunhye; Choi, Hyun Jin; Lee, Sungwook; Park, Boyoun.

In: PloS one, Vol. 9, No. 11, e112754, 14.11.2014.

Research output: Contribution to journalArticle

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