Differential cytokine responses of murine macrophage J774A.1 cells to stainless steel coated with and without hydroxyapatite

Mi Hee Lee, Dong Wook Han, Hyun Sook Baek, Hye Ryun Lim, In Seop Lee, Kwon Yong Lee, Kyung Tae Kim, Seung Jin Lee, Jong Chul Park

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Identification of cytokines secreted by macrophages and assessment of macrophage function in response to biomaterials is an important aspect of the host response to biomaterials. In the present study, differential cytokine responses of murine macrophage J774A.1 cells to stainless steel (SS) coated with and without hydroxyapatite (HA) were investigated. HA-coated SS was prepared through the deposition of thin (1 μm thick) calcium phosphate film by electron beam evaporation. SEM micrographs show that the surface of HA-coated SS was smoother than that of SS. Cell growth of J774A.1 macrophages onto HA-coated was about 1.5 times better than that onto intact SS. Reverse transcription-polymerase chain reaction analysis revealed that the mRNA of cytokines, such as tumor necrosis factor-α, transforming growth factor-β and granulocyte macrophage colony stimulating factor, was relatively less expressed in J774A.1 macrophages grown onto HA-coated SS. These results suggest that HA coatings may influence the expression of inflammatory cytokine in macrophages and improve the surface properties of metallic materials.

Original languageEnglish
Pages (from-to)5729-5732
Number of pages4
JournalSurface and Coatings Technology
Volume201
Issue number9-11 SPEC. ISS.
DOIs
Publication statusPublished - 2007 Feb 26

Fingerprint

macrophages
Macrophages
Stainless Steel
Durapatite
Hydroxyapatite
stainless steels
Stainless steel
Cytokines
cells
Biocompatible Materials
Biomaterials
Polymerase chain reaction
polymerase chain reaction
Cell growth
Transforming Growth Factors
Transcription
Granulocyte-Macrophage Colony-Stimulating Factor
calcium phosphates
necrosis
Calcium phosphate

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films
  • Materials Chemistry

Cite this

Lee, Mi Hee ; Han, Dong Wook ; Baek, Hyun Sook ; Lim, Hye Ryun ; Lee, In Seop ; Lee, Kwon Yong ; Kim, Kyung Tae ; Lee, Seung Jin ; Park, Jong Chul. / Differential cytokine responses of murine macrophage J774A.1 cells to stainless steel coated with and without hydroxyapatite. In: Surface and Coatings Technology. 2007 ; Vol. 201, No. 9-11 SPEC. ISS. pp. 5729-5732.
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Differential cytokine responses of murine macrophage J774A.1 cells to stainless steel coated with and without hydroxyapatite. / Lee, Mi Hee; Han, Dong Wook; Baek, Hyun Sook; Lim, Hye Ryun; Lee, In Seop; Lee, Kwon Yong; Kim, Kyung Tae; Lee, Seung Jin; Park, Jong Chul.

In: Surface and Coatings Technology, Vol. 201, No. 9-11 SPEC. ISS., 26.02.2007, p. 5729-5732.

Research output: Contribution to journalArticle

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AU - Lee, Mi Hee

AU - Han, Dong Wook

AU - Baek, Hyun Sook

AU - Lim, Hye Ryun

AU - Lee, In Seop

AU - Lee, Kwon Yong

AU - Kim, Kyung Tae

AU - Lee, Seung Jin

AU - Park, Jong Chul

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AB - Identification of cytokines secreted by macrophages and assessment of macrophage function in response to biomaterials is an important aspect of the host response to biomaterials. In the present study, differential cytokine responses of murine macrophage J774A.1 cells to stainless steel (SS) coated with and without hydroxyapatite (HA) were investigated. HA-coated SS was prepared through the deposition of thin (1 μm thick) calcium phosphate film by electron beam evaporation. SEM micrographs show that the surface of HA-coated SS was smoother than that of SS. Cell growth of J774A.1 macrophages onto HA-coated was about 1.5 times better than that onto intact SS. Reverse transcription-polymerase chain reaction analysis revealed that the mRNA of cytokines, such as tumor necrosis factor-α, transforming growth factor-β and granulocyte macrophage colony stimulating factor, was relatively less expressed in J774A.1 macrophages grown onto HA-coated SS. These results suggest that HA coatings may influence the expression of inflammatory cytokine in macrophages and improve the surface properties of metallic materials.

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