Differential effects of topical corticosteroid and calcineurin inhibitor on the epidermal tight junction

Sang E. Lee, Yuri Choi, Song Ee Kim, Eun B. Noh, SooChan Kim

Research output: Contribution to journalLetter

14 Citations (Scopus)

Abstract

Tight junction (TJ) is one of the functional barriers present in the skin. Although topical corticosteroids and calcineurin inhibitors are used widely for atopic dermatitis, the effect of these agents on TJs has not been reported. We investigated the structural changes of TJs in mice skin after application of 0.05% clobetasol propionate or 0.1% tacrolimus ointment for 10 days. Clobetasol caused epidermal thinning and decreased collagen density. Basal transepidermal water loss was significantly increased in clobetasol-treated versus vehicle- or tacrolimus-treated skin. Confocal immunofluorescence showed that clobetasol altered the structure of claudin-1,-4 and occludin. Tacrolimus also caused morphological alteration of occludin. Western blot and real-time PCR revealed that clobetasol significantly decreased claudin-1,-4 and occludin, whereas tacrolimus did not significantly affect claudin-1 and -4 but downregulated occludin to a lesser extent compared to clobetasol. In conclusion, we suggest that downregulation of TJ proteins expression is another pathomechanism of corticosteroid-induced permeability barrier disruption.

Original languageEnglish
Pages (from-to)59-61
Number of pages3
JournalExperimental dermatology
Volume22
Issue number1
DOIs
Publication statusPublished - 2013 Jan 1

Fingerprint

Clobetasol
Tight Junctions
Occludin
Adrenal Cortex Hormones
Claudin-4
Claudin-1
Tacrolimus
Skin
Down-Regulation
Tight Junction Proteins
Atopic Dermatitis
Ointments
Fluorescent Antibody Technique
Calcineurin Inhibitors
Real-Time Polymerase Chain Reaction
Permeability
Collagen
Western Blotting
Water

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology

Cite this

Lee, Sang E. ; Choi, Yuri ; Kim, Song Ee ; Noh, Eun B. ; Kim, SooChan. / Differential effects of topical corticosteroid and calcineurin inhibitor on the epidermal tight junction. In: Experimental dermatology. 2013 ; Vol. 22, No. 1. pp. 59-61.
@article{a6a6259cd2e942378a5f6acd030c434e,
title = "Differential effects of topical corticosteroid and calcineurin inhibitor on the epidermal tight junction",
abstract = "Tight junction (TJ) is one of the functional barriers present in the skin. Although topical corticosteroids and calcineurin inhibitors are used widely for atopic dermatitis, the effect of these agents on TJs has not been reported. We investigated the structural changes of TJs in mice skin after application of 0.05{\%} clobetasol propionate or 0.1{\%} tacrolimus ointment for 10 days. Clobetasol caused epidermal thinning and decreased collagen density. Basal transepidermal water loss was significantly increased in clobetasol-treated versus vehicle- or tacrolimus-treated skin. Confocal immunofluorescence showed that clobetasol altered the structure of claudin-1,-4 and occludin. Tacrolimus also caused morphological alteration of occludin. Western blot and real-time PCR revealed that clobetasol significantly decreased claudin-1,-4 and occludin, whereas tacrolimus did not significantly affect claudin-1 and -4 but downregulated occludin to a lesser extent compared to clobetasol. In conclusion, we suggest that downregulation of TJ proteins expression is another pathomechanism of corticosteroid-induced permeability barrier disruption.",
author = "Lee, {Sang E.} and Yuri Choi and Kim, {Song Ee} and Noh, {Eun B.} and SooChan Kim",
year = "2013",
month = "1",
day = "1",
doi = "10.1111/exd.12055",
language = "English",
volume = "22",
pages = "59--61",
journal = "Experimental Dermatology",
issn = "0906-6705",
publisher = "Wiley-Blackwell",
number = "1",

}

Differential effects of topical corticosteroid and calcineurin inhibitor on the epidermal tight junction. / Lee, Sang E.; Choi, Yuri; Kim, Song Ee; Noh, Eun B.; Kim, SooChan.

In: Experimental dermatology, Vol. 22, No. 1, 01.01.2013, p. 59-61.

Research output: Contribution to journalLetter

TY - JOUR

T1 - Differential effects of topical corticosteroid and calcineurin inhibitor on the epidermal tight junction

AU - Lee, Sang E.

AU - Choi, Yuri

AU - Kim, Song Ee

AU - Noh, Eun B.

AU - Kim, SooChan

PY - 2013/1/1

Y1 - 2013/1/1

N2 - Tight junction (TJ) is one of the functional barriers present in the skin. Although topical corticosteroids and calcineurin inhibitors are used widely for atopic dermatitis, the effect of these agents on TJs has not been reported. We investigated the structural changes of TJs in mice skin after application of 0.05% clobetasol propionate or 0.1% tacrolimus ointment for 10 days. Clobetasol caused epidermal thinning and decreased collagen density. Basal transepidermal water loss was significantly increased in clobetasol-treated versus vehicle- or tacrolimus-treated skin. Confocal immunofluorescence showed that clobetasol altered the structure of claudin-1,-4 and occludin. Tacrolimus also caused morphological alteration of occludin. Western blot and real-time PCR revealed that clobetasol significantly decreased claudin-1,-4 and occludin, whereas tacrolimus did not significantly affect claudin-1 and -4 but downregulated occludin to a lesser extent compared to clobetasol. In conclusion, we suggest that downregulation of TJ proteins expression is another pathomechanism of corticosteroid-induced permeability barrier disruption.

AB - Tight junction (TJ) is one of the functional barriers present in the skin. Although topical corticosteroids and calcineurin inhibitors are used widely for atopic dermatitis, the effect of these agents on TJs has not been reported. We investigated the structural changes of TJs in mice skin after application of 0.05% clobetasol propionate or 0.1% tacrolimus ointment for 10 days. Clobetasol caused epidermal thinning and decreased collagen density. Basal transepidermal water loss was significantly increased in clobetasol-treated versus vehicle- or tacrolimus-treated skin. Confocal immunofluorescence showed that clobetasol altered the structure of claudin-1,-4 and occludin. Tacrolimus also caused morphological alteration of occludin. Western blot and real-time PCR revealed that clobetasol significantly decreased claudin-1,-4 and occludin, whereas tacrolimus did not significantly affect claudin-1 and -4 but downregulated occludin to a lesser extent compared to clobetasol. In conclusion, we suggest that downregulation of TJ proteins expression is another pathomechanism of corticosteroid-induced permeability barrier disruption.

UR - http://www.scopus.com/inward/record.url?scp=84871713059&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84871713059&partnerID=8YFLogxK

U2 - 10.1111/exd.12055

DO - 10.1111/exd.12055

M3 - Letter

VL - 22

SP - 59

EP - 61

JO - Experimental Dermatology

JF - Experimental Dermatology

SN - 0906-6705

IS - 1

ER -