Differential expression and localization of connexins 26 and 43 in the rat gingival epithelium

Takashi Muramatsu, Tomoko Uekusa, Takayasu Masaoka, Masato Saitoh, Sadamitsu Hashimoto, Yoshihiro Abiko, Han Sung Jung, Masaki Shimono

Research output: Contribution to journalArticle

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Abstract

We investigated the expression and localization of connexins (CX) 26 and 43 in the rat gingival epithelium. RT-PCR analysis revealed CX26 gene expression in both the upper and lower layers of the gingival epithelium and in the total epithelial layer, whereas CX43 gene expression was limited to the lower layer and the total epithelial layer. Immunoreactivity for CX43 was observed in the membranes of adjacent cells from the basal layer to the middle of the prickle cell layer, while immunoreactivity for CX26 was observed in the granular cell layer and lower part of the squamous cell layer. Merged images revealed the co-localization of CX26 and CX43 in the middle of the prickle cell layer. By immuno-electron microscopy, gap junctions appeared curved, hemi-circular, or annular within the cytoplasm, and gold particles indicating the presence of CX43 were localized at the outer edges of these cytoplasmic formations. These results suggest that CX43 is associated with the regulation of cell proliferation and that increased CX26 expression is associated with differentiation of keratinocytes. Thus, degradation of CX43 is considered to play an essential role in differentiation of the rat gingival epithelium.

Original languageEnglish
Pages (from-to)147-154
Number of pages8
JournalArchives of Histology and Cytology
Volume71
Issue number3
DOIs
Publication statusPublished - 2008 Dec 1

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Connexin 43
Epithelium
Gene Expression
Immunoelectron Microscopy
Gap Junctions
Connexin 26
Keratinocytes
Gold
Cytoplasm
Epithelial Cells
Cell Proliferation
Cell Membrane
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Histology

Cite this

Muramatsu, T., Uekusa, T., Masaoka, T., Saitoh, M., Hashimoto, S., Abiko, Y., ... Shimono, M. (2008). Differential expression and localization of connexins 26 and 43 in the rat gingival epithelium. Archives of Histology and Cytology, 71(3), 147-154. https://doi.org/10.1679/aohc.71.147
Muramatsu, Takashi ; Uekusa, Tomoko ; Masaoka, Takayasu ; Saitoh, Masato ; Hashimoto, Sadamitsu ; Abiko, Yoshihiro ; Jung, Han Sung ; Shimono, Masaki. / Differential expression and localization of connexins 26 and 43 in the rat gingival epithelium. In: Archives of Histology and Cytology. 2008 ; Vol. 71, No. 3. pp. 147-154.
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Muramatsu, T, Uekusa, T, Masaoka, T, Saitoh, M, Hashimoto, S, Abiko, Y, Jung, HS & Shimono, M 2008, 'Differential expression and localization of connexins 26 and 43 in the rat gingival epithelium', Archives of Histology and Cytology, vol. 71, no. 3, pp. 147-154. https://doi.org/10.1679/aohc.71.147

Differential expression and localization of connexins 26 and 43 in the rat gingival epithelium. / Muramatsu, Takashi; Uekusa, Tomoko; Masaoka, Takayasu; Saitoh, Masato; Hashimoto, Sadamitsu; Abiko, Yoshihiro; Jung, Han Sung; Shimono, Masaki.

In: Archives of Histology and Cytology, Vol. 71, No. 3, 01.12.2008, p. 147-154.

Research output: Contribution to journalArticle

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