Differential expression of foxo genes during embryonic development and in adult tissues of Xenopus tropicalis

Li Zheng, Cheng Zhou Mao, Yun Qian Bi, Yi Min Zhou, Zhou Zhang, Hui Zhao, Kyu Sang Park, Ruijin Huang, Dong Qing Cai, Xu Feng Qi

Research output: Contribution to journalArticle

Abstract

The forkhead-box transcription factors of O subfamily (FOXO) play important roles in regulation of various biological functions. We cloned foxo1, foxo3, foxo4, and foxo6 from Xenopus tropicalis (hereafter X. tropicalis), and examined their expression in embryos and adult tissues. Maternal transcripts of foxo1 and foxo3 genes are detected within the animal half of the early embryo, their zygotic transcripts show distinct patterns. At late tailbud stages, foxo1 expression is observed mainly in eye, brain, branchial arches, and pronephros. In addition to eye, brain, branchial arches and pronephros, foxo3 expression is also evident in heart and somites. Foxo4 expression was not detected in oocytes. At late tailbud stages, foxo4 is mainly expressed in eye, brain, branchial arches and otic vesicle. Foxo6 expression was not detectable until stage 36, with a specific expression in nasal pits. Obvious expression of foxo1, foxo3 and foxo4, but not foxo6, is detected by RT-PCR both in oocytes and in embryos at examined stages. The expression of foxo1, foxo3 and foxo4 is observed in all tested adult tissues including heart, muscle, liver, lung, stomach and small intestine, while foxo6 is only detectable in stomach and small intestine. The differential expression pattern of foxo genes suggests that they exert distinct functions during embryonic development and in various organs of X. tropicalis.

Original languageEnglish
Article number119091
JournalGene Expression Patterns
Volume35
DOIs
Publication statusPublished - 2020 Jan

Fingerprint

Branchial Region
Pronephros
Xenopus
Embryonic Development
Embryonic Structures
Gene Expression
Small Intestine
Oocytes
Stomach
Brain
Forkhead Transcription Factors
Somites
Nose
Genes
Ear
Myocardium
Mothers
Polymerase Chain Reaction
Lung
Liver

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Developmental Biology

Cite this

Zheng, Li ; Mao, Cheng Zhou ; Bi, Yun Qian ; Zhou, Yi Min ; Zhang, Zhou ; Zhao, Hui ; Park, Kyu Sang ; Huang, Ruijin ; Cai, Dong Qing ; Qi, Xu Feng. / Differential expression of foxo genes during embryonic development and in adult tissues of Xenopus tropicalis. In: Gene Expression Patterns. 2020 ; Vol. 35.
@article{adf128dc26164aa5a1bdb71202156f33,
title = "Differential expression of foxo genes during embryonic development and in adult tissues of Xenopus tropicalis",
abstract = "The forkhead-box transcription factors of O subfamily (FOXO) play important roles in regulation of various biological functions. We cloned foxo1, foxo3, foxo4, and foxo6 from Xenopus tropicalis (hereafter X. tropicalis), and examined their expression in embryos and adult tissues. Maternal transcripts of foxo1 and foxo3 genes are detected within the animal half of the early embryo, their zygotic transcripts show distinct patterns. At late tailbud stages, foxo1 expression is observed mainly in eye, brain, branchial arches, and pronephros. In addition to eye, brain, branchial arches and pronephros, foxo3 expression is also evident in heart and somites. Foxo4 expression was not detected in oocytes. At late tailbud stages, foxo4 is mainly expressed in eye, brain, branchial arches and otic vesicle. Foxo6 expression was not detectable until stage 36, with a specific expression in nasal pits. Obvious expression of foxo1, foxo3 and foxo4, but not foxo6, is detected by RT-PCR both in oocytes and in embryos at examined stages. The expression of foxo1, foxo3 and foxo4 is observed in all tested adult tissues including heart, muscle, liver, lung, stomach and small intestine, while foxo6 is only detectable in stomach and small intestine. The differential expression pattern of foxo genes suggests that they exert distinct functions during embryonic development and in various organs of X. tropicalis.",
author = "Li Zheng and Mao, {Cheng Zhou} and Bi, {Yun Qian} and Zhou, {Yi Min} and Zhou Zhang and Hui Zhao and Park, {Kyu Sang} and Ruijin Huang and Cai, {Dong Qing} and Qi, {Xu Feng}",
year = "2020",
month = "1",
doi = "10.1016/j.gep.2019.119091",
language = "English",
volume = "35",
journal = "Brain research. Gene expression patterns",
issn = "1567-133X",
publisher = "Elsevier",

}

Differential expression of foxo genes during embryonic development and in adult tissues of Xenopus tropicalis. / Zheng, Li; Mao, Cheng Zhou; Bi, Yun Qian; Zhou, Yi Min; Zhang, Zhou; Zhao, Hui; Park, Kyu Sang; Huang, Ruijin; Cai, Dong Qing; Qi, Xu Feng.

In: Gene Expression Patterns, Vol. 35, 119091, 01.2020.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Differential expression of foxo genes during embryonic development and in adult tissues of Xenopus tropicalis

AU - Zheng, Li

AU - Mao, Cheng Zhou

AU - Bi, Yun Qian

AU - Zhou, Yi Min

AU - Zhang, Zhou

AU - Zhao, Hui

AU - Park, Kyu Sang

AU - Huang, Ruijin

AU - Cai, Dong Qing

AU - Qi, Xu Feng

PY - 2020/1

Y1 - 2020/1

N2 - The forkhead-box transcription factors of O subfamily (FOXO) play important roles in regulation of various biological functions. We cloned foxo1, foxo3, foxo4, and foxo6 from Xenopus tropicalis (hereafter X. tropicalis), and examined their expression in embryos and adult tissues. Maternal transcripts of foxo1 and foxo3 genes are detected within the animal half of the early embryo, their zygotic transcripts show distinct patterns. At late tailbud stages, foxo1 expression is observed mainly in eye, brain, branchial arches, and pronephros. In addition to eye, brain, branchial arches and pronephros, foxo3 expression is also evident in heart and somites. Foxo4 expression was not detected in oocytes. At late tailbud stages, foxo4 is mainly expressed in eye, brain, branchial arches and otic vesicle. Foxo6 expression was not detectable until stage 36, with a specific expression in nasal pits. Obvious expression of foxo1, foxo3 and foxo4, but not foxo6, is detected by RT-PCR both in oocytes and in embryos at examined stages. The expression of foxo1, foxo3 and foxo4 is observed in all tested adult tissues including heart, muscle, liver, lung, stomach and small intestine, while foxo6 is only detectable in stomach and small intestine. The differential expression pattern of foxo genes suggests that they exert distinct functions during embryonic development and in various organs of X. tropicalis.

AB - The forkhead-box transcription factors of O subfamily (FOXO) play important roles in regulation of various biological functions. We cloned foxo1, foxo3, foxo4, and foxo6 from Xenopus tropicalis (hereafter X. tropicalis), and examined their expression in embryos and adult tissues. Maternal transcripts of foxo1 and foxo3 genes are detected within the animal half of the early embryo, their zygotic transcripts show distinct patterns. At late tailbud stages, foxo1 expression is observed mainly in eye, brain, branchial arches, and pronephros. In addition to eye, brain, branchial arches and pronephros, foxo3 expression is also evident in heart and somites. Foxo4 expression was not detected in oocytes. At late tailbud stages, foxo4 is mainly expressed in eye, brain, branchial arches and otic vesicle. Foxo6 expression was not detectable until stage 36, with a specific expression in nasal pits. Obvious expression of foxo1, foxo3 and foxo4, but not foxo6, is detected by RT-PCR both in oocytes and in embryos at examined stages. The expression of foxo1, foxo3 and foxo4 is observed in all tested adult tissues including heart, muscle, liver, lung, stomach and small intestine, while foxo6 is only detectable in stomach and small intestine. The differential expression pattern of foxo genes suggests that they exert distinct functions during embryonic development and in various organs of X. tropicalis.

UR - http://www.scopus.com/inward/record.url?scp=85075427759&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85075427759&partnerID=8YFLogxK

U2 - 10.1016/j.gep.2019.119091

DO - 10.1016/j.gep.2019.119091

M3 - Article

C2 - 31770608

AN - SCOPUS:85075427759

VL - 35

JO - Brain research. Gene expression patterns

JF - Brain research. Gene expression patterns

SN - 1567-133X

M1 - 119091

ER -