Differential expression of nephrin according to glomerular size in early diabetic kidney disease

Jin Ju Kim, Ji Li Jin, Dong Sub Jung, Seung Jae Kwak, Dong Ryeol Ryu, TaeHyun Yoo, SeungHyeok Han, Young Choi Hoon, Jong Kim Hyung, Suk Han Dae, Shin-Wook Kang

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Diabetic nephropathy (DN) is clinically characterized by proteinuria. Many studies tried to demonstrate a relationship between proteinuria and changes in nephrin in various forms of glomerular diseases including DN, but the results are not consistent. Glomerular hypertrophy occurs in DN, yet hypertrophy does not develop in all glomeruli concurrently. For investigation of the differences in nephrin expression according to glomerular size, glomeruli were isolated from 10 control and 10 streptozotocin-induced diabetic rats at 6 wk after the induction of diabetes by a sieving technique using sieves with pore sizes of 250, 150, 125, and 75 μm. Glomeruli then were classified into large glomeruli (LG; on the 125-μm sieve) and small glomeruli (SG; on the 75-μm sieve) groups. Glomerular volumes were determined using an image analyzer, and mRNA and protein expression was determined by real-time PCR and Western blot, respectively. The mean volumes of diabetic LG (1.51 ± 0.06 × 106 μm3) and control LG (1.37 ± 0.05 × 106 μm3) were significantly higher than those of diabetic SG (0.94 ± 0.03 × 106 μm3) and control SG (0.87 ± 0.03 × 106 μm3; P < 0.01). Nephrin mRNA expression was significantly reduced in the diabetic LG group compared with the diabetic SG and control glomeruli groups (P < 0.05). In contrast, nephrin mRNA expression was significantly higher in the diabetic SG group compared with the diabetic LG and control glomeruli groups (P < 0.05). Even after correction for 18s rRNA and Wilms' tumor-1 mRNA expression, the differences in nephrin mRNA expression remained significant. The expression of nephrin protein showed a similar pattern to the mRNA expression. In conclusion, these data suggest that the nephrin gene is differentially expressed according to glomerular size. Furthermore, more hypertrophied glomeruli with lesser nephrin expression may be responsible for albuminuria in the early stage of DN.

Original languageEnglish
Pages (from-to)2303-2310
Number of pages8
JournalJournal of the American Society of Nephrology
Volume18
Issue number8
DOIs
Publication statusPublished - 2007 Aug 1

Fingerprint

Diabetic Nephropathies
Messenger RNA
Proteinuria
Hypertrophy
Nicorandil
Control Groups
Albuminuria
Wilms Tumor
nephrin
Streptozocin
Real-Time Polymerase Chain Reaction
Proteins
Western Blotting
Genes

All Science Journal Classification (ASJC) codes

  • Nephrology

Cite this

Kim, Jin Ju ; Jin, Ji Li ; Jung, Dong Sub ; Kwak, Seung Jae ; Ryu, Dong Ryeol ; Yoo, TaeHyun ; Han, SeungHyeok ; Hoon, Young Choi ; Hyung, Jong Kim ; Dae, Suk Han ; Kang, Shin-Wook. / Differential expression of nephrin according to glomerular size in early diabetic kidney disease. In: Journal of the American Society of Nephrology. 2007 ; Vol. 18, No. 8. pp. 2303-2310.
@article{e8ebde63cada496fb77df1feaa433358,
title = "Differential expression of nephrin according to glomerular size in early diabetic kidney disease",
abstract = "Diabetic nephropathy (DN) is clinically characterized by proteinuria. Many studies tried to demonstrate a relationship between proteinuria and changes in nephrin in various forms of glomerular diseases including DN, but the results are not consistent. Glomerular hypertrophy occurs in DN, yet hypertrophy does not develop in all glomeruli concurrently. For investigation of the differences in nephrin expression according to glomerular size, glomeruli were isolated from 10 control and 10 streptozotocin-induced diabetic rats at 6 wk after the induction of diabetes by a sieving technique using sieves with pore sizes of 250, 150, 125, and 75 μm. Glomeruli then were classified into large glomeruli (LG; on the 125-μm sieve) and small glomeruli (SG; on the 75-μm sieve) groups. Glomerular volumes were determined using an image analyzer, and mRNA and protein expression was determined by real-time PCR and Western blot, respectively. The mean volumes of diabetic LG (1.51 ± 0.06 × 106 μm3) and control LG (1.37 ± 0.05 × 106 μm3) were significantly higher than those of diabetic SG (0.94 ± 0.03 × 106 μm3) and control SG (0.87 ± 0.03 × 106 μm3; P < 0.01). Nephrin mRNA expression was significantly reduced in the diabetic LG group compared with the diabetic SG and control glomeruli groups (P < 0.05). In contrast, nephrin mRNA expression was significantly higher in the diabetic SG group compared with the diabetic LG and control glomeruli groups (P < 0.05). Even after correction for 18s rRNA and Wilms' tumor-1 mRNA expression, the differences in nephrin mRNA expression remained significant. The expression of nephrin protein showed a similar pattern to the mRNA expression. In conclusion, these data suggest that the nephrin gene is differentially expressed according to glomerular size. Furthermore, more hypertrophied glomeruli with lesser nephrin expression may be responsible for albuminuria in the early stage of DN.",
author = "Kim, {Jin Ju} and Jin, {Ji Li} and Jung, {Dong Sub} and Kwak, {Seung Jae} and Ryu, {Dong Ryeol} and TaeHyun Yoo and SeungHyeok Han and Hoon, {Young Choi} and Hyung, {Jong Kim} and Dae, {Suk Han} and Shin-Wook Kang",
year = "2007",
month = "8",
day = "1",
doi = "10.1681/ASN.2006101145",
language = "English",
volume = "18",
pages = "2303--2310",
journal = "Journal of the American Society of Nephrology : JASN",
issn = "1046-6673",
publisher = "American Society of Nephrology",
number = "8",

}

Differential expression of nephrin according to glomerular size in early diabetic kidney disease. / Kim, Jin Ju; Jin, Ji Li; Jung, Dong Sub; Kwak, Seung Jae; Ryu, Dong Ryeol; Yoo, TaeHyun; Han, SeungHyeok; Hoon, Young Choi; Hyung, Jong Kim; Dae, Suk Han; Kang, Shin-Wook.

In: Journal of the American Society of Nephrology, Vol. 18, No. 8, 01.08.2007, p. 2303-2310.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Differential expression of nephrin according to glomerular size in early diabetic kidney disease

AU - Kim, Jin Ju

AU - Jin, Ji Li

AU - Jung, Dong Sub

AU - Kwak, Seung Jae

AU - Ryu, Dong Ryeol

AU - Yoo, TaeHyun

AU - Han, SeungHyeok

AU - Hoon, Young Choi

AU - Hyung, Jong Kim

AU - Dae, Suk Han

AU - Kang, Shin-Wook

PY - 2007/8/1

Y1 - 2007/8/1

N2 - Diabetic nephropathy (DN) is clinically characterized by proteinuria. Many studies tried to demonstrate a relationship between proteinuria and changes in nephrin in various forms of glomerular diseases including DN, but the results are not consistent. Glomerular hypertrophy occurs in DN, yet hypertrophy does not develop in all glomeruli concurrently. For investigation of the differences in nephrin expression according to glomerular size, glomeruli were isolated from 10 control and 10 streptozotocin-induced diabetic rats at 6 wk after the induction of diabetes by a sieving technique using sieves with pore sizes of 250, 150, 125, and 75 μm. Glomeruli then were classified into large glomeruli (LG; on the 125-μm sieve) and small glomeruli (SG; on the 75-μm sieve) groups. Glomerular volumes were determined using an image analyzer, and mRNA and protein expression was determined by real-time PCR and Western blot, respectively. The mean volumes of diabetic LG (1.51 ± 0.06 × 106 μm3) and control LG (1.37 ± 0.05 × 106 μm3) were significantly higher than those of diabetic SG (0.94 ± 0.03 × 106 μm3) and control SG (0.87 ± 0.03 × 106 μm3; P < 0.01). Nephrin mRNA expression was significantly reduced in the diabetic LG group compared with the diabetic SG and control glomeruli groups (P < 0.05). In contrast, nephrin mRNA expression was significantly higher in the diabetic SG group compared with the diabetic LG and control glomeruli groups (P < 0.05). Even after correction for 18s rRNA and Wilms' tumor-1 mRNA expression, the differences in nephrin mRNA expression remained significant. The expression of nephrin protein showed a similar pattern to the mRNA expression. In conclusion, these data suggest that the nephrin gene is differentially expressed according to glomerular size. Furthermore, more hypertrophied glomeruli with lesser nephrin expression may be responsible for albuminuria in the early stage of DN.

AB - Diabetic nephropathy (DN) is clinically characterized by proteinuria. Many studies tried to demonstrate a relationship between proteinuria and changes in nephrin in various forms of glomerular diseases including DN, but the results are not consistent. Glomerular hypertrophy occurs in DN, yet hypertrophy does not develop in all glomeruli concurrently. For investigation of the differences in nephrin expression according to glomerular size, glomeruli were isolated from 10 control and 10 streptozotocin-induced diabetic rats at 6 wk after the induction of diabetes by a sieving technique using sieves with pore sizes of 250, 150, 125, and 75 μm. Glomeruli then were classified into large glomeruli (LG; on the 125-μm sieve) and small glomeruli (SG; on the 75-μm sieve) groups. Glomerular volumes were determined using an image analyzer, and mRNA and protein expression was determined by real-time PCR and Western blot, respectively. The mean volumes of diabetic LG (1.51 ± 0.06 × 106 μm3) and control LG (1.37 ± 0.05 × 106 μm3) were significantly higher than those of diabetic SG (0.94 ± 0.03 × 106 μm3) and control SG (0.87 ± 0.03 × 106 μm3; P < 0.01). Nephrin mRNA expression was significantly reduced in the diabetic LG group compared with the diabetic SG and control glomeruli groups (P < 0.05). In contrast, nephrin mRNA expression was significantly higher in the diabetic SG group compared with the diabetic LG and control glomeruli groups (P < 0.05). Even after correction for 18s rRNA and Wilms' tumor-1 mRNA expression, the differences in nephrin mRNA expression remained significant. The expression of nephrin protein showed a similar pattern to the mRNA expression. In conclusion, these data suggest that the nephrin gene is differentially expressed according to glomerular size. Furthermore, more hypertrophied glomeruli with lesser nephrin expression may be responsible for albuminuria in the early stage of DN.

UR - http://www.scopus.com/inward/record.url?scp=34547616609&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34547616609&partnerID=8YFLogxK

U2 - 10.1681/ASN.2006101145

DO - 10.1681/ASN.2006101145

M3 - Article

VL - 18

SP - 2303

EP - 2310

JO - Journal of the American Society of Nephrology : JASN

JF - Journal of the American Society of Nephrology : JASN

SN - 1046-6673

IS - 8

ER -