Differential expression of phospholipase D isozymes in the hippocampus following kainic acid-induced seizures

Seong Yun Kim, Do Sik Min, Jeong Sun Choi, Yun Sik Choi, Hyun Jung Park, Ki Wug Sung, Joonmo Kim, Mun Yong Lee

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

To investigate the pathophysiological role of phospholipase D (PLD)-mediated signaling, changes in the expression of the PLD isozymes PLD1 and PLD2 were investigated in the rat kainic acid (KA) model of human temporal lobe epilepsy. Western blot analysis showed a significant increase in the expression of PLD1 and PLD2 in the postictal hippocampus. PLD1 immunoreactivity increased preferentially in the CA3 and CA1 regions, where pyramidal neurons are susceptible to temporal lobe epilepsy. Experiments employing double immunofluorescence revealed that the cells expressing PLD1 were GFAP-expressing reactive astrocytes. By contrast, PLD2 immunoreactivity increased strikingly in infrapyramidal, but not in suprapyramidal granule cells of the postictal dentate gyrus, fitting well with results of the PLD activity assay. Considering that PLD belongs to a key signaling pathway, this result suggests that changes in granule cell activity in the dentate gyrus after seizures occurs specifically between the supra- and infrapyramidal blades. In addition, enhanced immunoreactivity of PLD2 was observed in the reactive astrocytes of the CA1, CA3, and hilar subregions, but its temporal pattern is different from that of PLD1. Taken together, our results suggest that PLD1 and PLD2 exercise their unique pathophysiological functions in the rat hippocampus after KA-induced seizures.

Original languageEnglish
Pages (from-to)812-820
Number of pages9
JournalJournal of Neuropathology and Experimental Neurology
Volume63
Issue number8
DOIs
Publication statusPublished - 2004 Aug

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Phospholipase D
Kainic Acid
Isoenzymes
Hippocampus
Seizures
Temporal Lobe Epilepsy
Dentate Gyrus
Astrocytes
Pyramidal Cells
Fluorescent Antibody Technique
Western Blotting

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Neurology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

Cite this

Kim, Seong Yun ; Min, Do Sik ; Choi, Jeong Sun ; Choi, Yun Sik ; Park, Hyun Jung ; Sung, Ki Wug ; Kim, Joonmo ; Lee, Mun Yong. / Differential expression of phospholipase D isozymes in the hippocampus following kainic acid-induced seizures. In: Journal of Neuropathology and Experimental Neurology. 2004 ; Vol. 63, No. 8. pp. 812-820.
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Differential expression of phospholipase D isozymes in the hippocampus following kainic acid-induced seizures. / Kim, Seong Yun; Min, Do Sik; Choi, Jeong Sun; Choi, Yun Sik; Park, Hyun Jung; Sung, Ki Wug; Kim, Joonmo; Lee, Mun Yong.

In: Journal of Neuropathology and Experimental Neurology, Vol. 63, No. 8, 08.2004, p. 812-820.

Research output: Contribution to journalArticle

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