Differential expression of phospholipases D1 and D2 in mouse tissues

Heechul Kim, Jeeyoung Lee, Seungjoon Kim, Min Kyoung Shin, Do Sik Min, Taekyun Shin

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The differential expression of phospholipase D (PLD) isozymes, which include PLD1 and PLD2, was examined in various murine tissues, including the cerebrum, cerebellum, heart, lung, liver, spleen, stomach, pancreas, ileum, colon, adrenal gland, kidneys, testes, ovaries, and uterus. In Western blot analysis, only PLD1 was detected in the heart and ovary, while only PLD2 was detected in the pancreas and ileum. Both PLD1 and PLD2 were strongly expressed in the cerebrum, cerebellum, and lung, and both were also expressed in the liver, spleen, stomach, colon, kidney, testes, and uterus. Immunohistochemistry showed intense PLD immunostaining in the cerebrum, cerebellum, lungs, intestines, and testis, and weak PLD immunostaining in the liver, kidneys, spleen, and heart. These findings suggest that PLD1 and PLD2 are differentially expressed in the various organs of mice, and that each PLD isozyme plays a distinct role in each organ.

Original languageEnglish
Pages (from-to)148-155
Number of pages8
JournalCell Biology International
Volume31
Issue number2
DOIs
Publication statusPublished - 2007 Feb 1

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Phospholipase D
Cerebrum
Cerebellum
Testis
Spleen
Kidney
Ileum
Lung
Isoenzymes
Uterus
Pancreas
Ovary
Liver
Stomach
Colon
Adrenal Glands
Intestines
Western Blotting
Immunohistochemistry
phospholipase D2

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Kim, Heechul ; Lee, Jeeyoung ; Kim, Seungjoon ; Shin, Min Kyoung ; Min, Do Sik ; Shin, Taekyun. / Differential expression of phospholipases D1 and D2 in mouse tissues. In: Cell Biology International. 2007 ; Vol. 31, No. 2. pp. 148-155.
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Differential expression of phospholipases D1 and D2 in mouse tissues. / Kim, Heechul; Lee, Jeeyoung; Kim, Seungjoon; Shin, Min Kyoung; Min, Do Sik; Shin, Taekyun.

In: Cell Biology International, Vol. 31, No. 2, 01.02.2007, p. 148-155.

Research output: Contribution to journalArticle

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