Differential gel-based proteomic approach for cancer biomarker discovery using human plasma

Keun Na, Min Jung Lee, Hye Jin Jeong, Hoguen Kim, Young Ki Paik

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Two-dimensional fluorescence difference gel electrophoresis (2D DIGE) has become a general platform for analysis of various clinical samples such as biofluids and tissues. In comparison to conventional 2-D polyacrylamide gel electrophoresis (2D PAGE), 2D DIGE offers several advantages, such as accuracy and reproducibility between experiments, which facilitate spot-to-spot comparisons. Although whole plasma can be easily obtained, the complexity of plasma samples makes it challenging to analyze samples with good reproducibility. Here, we describe a method for decreasing protein complexity in plasma samples within a narrow pH range by depleting high-abundance plasma proteins. In combination with analysis of differentially expressed spots, trypsin digestion, identification of protein by mass spectrometry, and standard 2D PAGE and DIGE, this method has been optimized for comparison of plasma samples from healthy donors and patients diagnosed with hepatocellular carcinoma.

Original languageEnglish
Title of host publicationDifference Gel Electrophoresis (DIGE)
Subtitle of host publicationMethods and Protocols
Pages223-237
Number of pages15
DOIs
Publication statusPublished - 2012 Mar 16

Publication series

NameMethods in Molecular Biology
Volume854
ISSN (Print)1064-3745

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Na, K., Lee, M. J., Jeong, H. J., Kim, H., & Paik, Y. K. (2012). Differential gel-based proteomic approach for cancer biomarker discovery using human plasma. In Difference Gel Electrophoresis (DIGE): Methods and Protocols (pp. 223-237). (Methods in Molecular Biology; Vol. 854). https://doi.org/10.1007/978-1-61779-573-2_16