Differential inhibitory effects of protoberberines on sterol and chitin biosyntheses in Candida albicans

Kang Sik Park, Kap Chul Kang, Jai Hyun Kim, David J. Adams, Tae Neung Johng, Young Ki Paik

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Abstract

The anti-Candida potentials of 12 Korean medicinal plants were explored: methanol extracts from Coptis rhizoma and Phellodendron amurense caused significant inhibition of growth of Candida albicans, Candida glabrata, Candida krusei and Candida parapsilosis. The predominant active components of the extracts were the protoberberines berberine and palmatine; the most potent inhibition of growth was exhibited by berberine on C. krusei (MIC < 4 mg/L) and palmatine on C. parapsilosis (MIC 16 mg/L). Both berberine and palmatine inhibited the in-vivo rate of incorporation of L-[methyl/14C]methionine into C-24 of ergosterol in C. albicans (50% inhibition concentration (IC50 values), 25 μM and 300 μM, respectively); this result suggests that sterol 24-methyl transferase (24-SMT) is one of the cellular targets for the antifungal activity of the protoberberines. In-vitro 24-SMT activity in microsomes from the yeast growth form of C. albicans was inhibited by both berberine (inhibition constant (K(i)) 232 μM) and palmatine (K(i) 257 μM) in a non-competitive manner; inhibition of 24-SMT was more marked for the mycelial form than for the yeast growth form of this organism. Palmatine inhibited chitin synthase from both the yeast and mycelial growth phases of C. albicans in a non-competitive manner (K(i) 780 μM). The effects of protoberberines, extracted from established medicinal plants, on both sterol and cell wall biosyntheses in pathogenic fungi indicate that the potential of these compounds, or their semi-synthetic derivatives, as a novel class of antifungal agents should be investigated more fully.

Original languageEnglish
Pages (from-to)667-674
Number of pages8
JournalJournal of Antimicrobial Chemotherapy
Volume43
Issue number5
DOIs
Publication statusPublished - 1999 Jan 1

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All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

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