Differential physiological roles of ESCRT complexes in Caenorhabditis elegans

Dong Wan Kim, Hyun Sung, Donghyuk Shin, Haihong Shen, Joohong Ahnn, Sun Kyung Lee, Sangho Lee

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Endosomal sorting complex required for transport (ESCRT) complexes are involved in endosomal trafficking to the lysosome, cytokinesis, and viral budding. Extensive genetic, biochemical, and structural studies on the ESCRT system have been carried out in yeast and mammalian systems. However, the question of how the ESCRT system functions at the whole organism level has not been fully explored. In C. elegans, we performed RNAi experiments to knock-down gene expression of components of the ESCRT system and profiled their effects on protein degradation and endocytosis of YP170, a yolk protein. Targeted RNAi knock-down of ESCRT-I (tsg-101 and vps-28) and ESCRT-III (vps-24, and vps-32.2) components interfered with protein degradation while knock-down of ESCRT-II (vps-25 and vps-36) and ESCRT-III (vps-20 and vps-24) components hampered endocytosis. In contrast, the knockdown of vps-37, another ESCRT-I component, showed no defect in either YP170 uptake or degradation. Depletion of at least one component from each complex - ESCRT-0 (hgrs-1), ESCRT-I (tsg-101, vps-28, and vps-37), ESCRT-II (vps-36), ESCRT-III (vps-24), and Vps4 (vps-4) - resulted in abnormal distribution of embryos in the uterus of worms, possibly due to abnormal ovulation, fertilization, and egglaying. These results suggest differential physiological roles of ESCRT-0, -I, -II, and -III complexes in the context of the whole organism, C. elegans.

Original languageEnglish
Pages (from-to)585-592
Number of pages8
JournalMolecules and cells
Volume31
Issue number6
DOIs
Publication statusPublished - 2011 Jun

Bibliographical note

Funding Information:
We gratefully acknowledge the Caenorhabditis Genetic Center for pwIs23[vit-2::GFP]. We also thank Yeon-Woo Ju and Huijin Noh for their excellent technical assistance. This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government (KRF-2008-314-C00224).

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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