Differential regulation of NO availability from macrophages and endothelial cells by the garlic component S-allyl cysteine

Ki Mo Kim, Sun Bum Chun, Myoung Sook Koo, Won Jin Choi, Tae Woong Kim, Young-Guen Kwon, Hun Taeg Chung, Timothy R. Billiar, Young Myeong Kim

Research output: Contribution to journalArticle

163 Citations (Scopus)

Abstract

Garlic has been used as a traditional medicine for prevention and treatment of cardiovascular diseases. However, the molecular mechanism of garlic's pharmacological action has not been clearly elucidated. We examined here the effect of garlic extract and its major component, S-allyl cysteine (SAC), on nitric oxide (NO) production by macrophages and endothelial cells. The present study demonstrates that these reagents inhibited NO production through the suppression of iNOS mRNA and protein expression in the murine macrophage cell line RAW264.7, which had been stimulated with LPS and IFNγ. The garlic extract also inhibited NO production in peritoneal macrophages, rat hepatocytes, and rat aortic smooth muscle cells stimulated with LPS plus cytokines, but it did not inhibit NO production in iNOS-transfected AKN-1 cells or iNOS enzyme activity. These reagents suppressed NF-κB activation and murine iNOS promoter activity in LPS and IFNγ-stimulated RAW264.7 cells. In contrast, these reagents significantly increased cGMP production by eNOS in HUVEC without changes in activity, protein levels, and cellular distribution of eNOS. Finally, garlic extract and SAC both suppressed the production of hydroxyl radical, confirming their antioxidant activity. These data demonstrate that garlic extract and SAC, due to their antioxidant activity, differentially regulate NO production by inhibiting iNOS expression in macrophages while increasing NO in endothelial cells. Thus, this selective regulation may contribute to the anti-inflammatory effect and prevention of atherosclerosis by these reagents.

Original languageEnglish
Pages (from-to)747-756
Number of pages10
JournalFree Radical Biology and Medicine
Volume30
Issue number7
DOIs
Publication statusPublished - 2001 Apr 1

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Garlic
Macrophages
Endothelial cells
Cellular Structures
Nitric Oxide
Endothelial Cells
Availability
Molecular Mechanisms of Pharmacological Action
Antioxidants
Rats
Cells
Peritoneal Macrophages
Traditional Medicine
Hydroxyl Radical
Enzyme activity
Smooth Muscle Myocytes
S-allylcysteine
Hepatocytes
Atherosclerosis
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology (medical)

Cite this

Kim, Ki Mo ; Chun, Sun Bum ; Koo, Myoung Sook ; Choi, Won Jin ; Kim, Tae Woong ; Kwon, Young-Guen ; Chung, Hun Taeg ; Billiar, Timothy R. ; Kim, Young Myeong. / Differential regulation of NO availability from macrophages and endothelial cells by the garlic component S-allyl cysteine. In: Free Radical Biology and Medicine. 2001 ; Vol. 30, No. 7. pp. 747-756.
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Differential regulation of NO availability from macrophages and endothelial cells by the garlic component S-allyl cysteine. / Kim, Ki Mo; Chun, Sun Bum; Koo, Myoung Sook; Choi, Won Jin; Kim, Tae Woong; Kwon, Young-Guen; Chung, Hun Taeg; Billiar, Timothy R.; Kim, Young Myeong.

In: Free Radical Biology and Medicine, Vol. 30, No. 7, 01.04.2001, p. 747-756.

Research output: Contribution to journalArticle

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