Differential transmission of G1 cell cycle arrest and mating signals by saccharomyces cerevisiae Ste5 mutants in the pheromone pathway

You Jeong Choi, Sun Hong Kim, Ki Sook Park, Kang-Yell Choi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Saccharomyces cerevisiae Ste5 is a scaffold protein that recruits many pheromone signaling molecules to sequester the pheromone pathway from other homologous mitogen-activated protein kinase pathways. G1 cell cycle arrest and mating are two different physiological consequences of pheromone signal transduction and Ste5 is required for both processes. However, the roles of Ste5 in G1 arrest and mating are not fully understood. To understand the roles of Ste5 better, we isolated 150 G1 cell cycle arrest defective STE5 mutants by chemical mutagenesis of the gene. Here, we found that two G1 cell cycle arrest defective STE5 mutants (ste5M(D248V) and ste5(Δ-776)) retained mating capacity. When overproduced in a wild-type strain, several ste5 mutants also showed different dominant phenotypes for G1 arrest and mating. Isolation and characterization of the mutants suggested separable roles of Ste5 in G1 arrest and mating of S. cerevisiae. In addition, the roles of Asp- 248 and Tyr-421, which are important for pheromone signal transduction were further characterized by site-directed mutagenesis studies.

Original languageEnglish
Pages (from-to)459-468
Number of pages10
JournalBiochemistry and Cell Biology
Volume77
Issue number5
Publication statusPublished - 1999 Dec 1

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G1 Phase Cell Cycle Checkpoints
Pheromones
Yeast
Saccharomyces cerevisiae
Cells
Signal transduction
Mutagenesis
Signal Transduction
Site-Directed Mutagenesis
Mitogen-Activated Protein Kinases
Scaffolds
Genes
Phenotype
Molecules
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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title = "Differential transmission of G1 cell cycle arrest and mating signals by saccharomyces cerevisiae Ste5 mutants in the pheromone pathway",
abstract = "Saccharomyces cerevisiae Ste5 is a scaffold protein that recruits many pheromone signaling molecules to sequester the pheromone pathway from other homologous mitogen-activated protein kinase pathways. G1 cell cycle arrest and mating are two different physiological consequences of pheromone signal transduction and Ste5 is required for both processes. However, the roles of Ste5 in G1 arrest and mating are not fully understood. To understand the roles of Ste5 better, we isolated 150 G1 cell cycle arrest defective STE5 mutants by chemical mutagenesis of the gene. Here, we found that two G1 cell cycle arrest defective STE5 mutants (ste5M(D248V) and ste5(Δ-776)) retained mating capacity. When overproduced in a wild-type strain, several ste5 mutants also showed different dominant phenotypes for G1 arrest and mating. Isolation and characterization of the mutants suggested separable roles of Ste5 in G1 arrest and mating of S. cerevisiae. In addition, the roles of Asp- 248 and Tyr-421, which are important for pheromone signal transduction were further characterized by site-directed mutagenesis studies.",
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Differential transmission of G1 cell cycle arrest and mating signals by saccharomyces cerevisiae Ste5 mutants in the pheromone pathway. / Choi, You Jeong; Kim, Sun Hong; Park, Ki Sook; Choi, Kang-Yell.

In: Biochemistry and Cell Biology, Vol. 77, No. 5, 01.12.1999, p. 459-468.

Research output: Contribution to journalArticle

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