Diffusive and directional intracellular dynamics measured by field-based dynamic light scattering

Chulmin Joo, Conor L. Evans, Thomas Stepinac, Tayyaba Hasan, Johannes F. De Boer

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Quantitative measurement of diffusive and directional processes of intracellular structures is not only critical in understanding cell mechanics and functions, but also has many applications, such as investigation of cellular responses to therapeutic agents. We introduce a label-free optical technique that allows non-perturbative characterization of localized intracellular dynamics. The method combines a field-based dynamic light scattering analysis with a confocal interferometric microscope to provide a statistical measure of the diffusive and directional motion of scattering structures inside a microscopic probe volume. To demonstrate the potential of this technique, we examined the localized intracellular dynamics in human epithelial ovarian cancer cells. We observed the distinctive temporal regimes of intracellular dynamics, which transitions from random to directional processes on a timescale of ∼0.01 sec. In addition, we observed disrupted directional processes on the timescale of 1∼5 sec by the application of a microtubule polymerization inhibitor, Colchicine, and ATP depletion.

Original languageEnglish
Pages (from-to)2858-2871
Number of pages14
JournalOptics Express
Volume18
Issue number3
DOIs
Publication statusPublished - 2010 Feb 1

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

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