Dimethyl 3,3″-dithiobispropionimidate (DTBP) as a cleavable disulfide-based polymer to encapsulate nucleic acids in biological sample preparation

Huifang Liu, Fei Zhao, Bonhan Koo, Yange Luan, Linlin Zhong, Kyusik Yun, Yong Shin

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Cleavable disulfide bonds are extremely valuable functional groups for numerous biological applications, including applications as antitumor treatments, gene delivery, and diagnosis. Herein, we demonstrate the use of dimethyl 3,3′-dithiobispropionimidate (DTBP) as a disulfide cleavable bond based cross-linker in nano-composites that encapsulate and release nucleic acids in sample preparation systems for disease diagnoses. We conjugated DTBP with nano-composites, such as amine group-modified diatomaceous earth (DA), which is a solid substrate, and a zinc oxide nanomultigonal shuttle (ZnO NMS) that disrupts cell membranes without chaotropic detergents and improved the quantity and purity of nucleic acids from various biological samples. After encapsulating nucleic acids, the cleavable disulfide bond of DTBP was used to release nucleic acids by breaking disulfide bonds using dithiothreitol (DTT), resulting in highly enhanced efficiency of downstream analyses without PCR inhibitors such as high salt concentrations and SDS. Therefore, nano-composites with cleavable disulfide linkages have potential for various biological applications, including disease diagnostics and therapies.

Original languageEnglish
Pages (from-to)225-231
Number of pages7
JournalSensors and Actuators, B: Chemical
Volume288
DOIs
Publication statusPublished - 2019 Jun 1

Bibliographical note

Funding Information:
This work was supported by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute, funded by the Ministry of Health & Welfare, Republic of Korea ( HI16C-0272-010016 ), and also supported by a grant ( 2018-701 ) from the Asan Institute for Life Sciences, Asan Medical Center, Seoul, Republic of Korea .

Publisher Copyright:
© 2019

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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