Direct detection of rifampin-resistant mycobacterium tuberculosis in respiratory specimens using Quantamatrix Multiplexed Assay Platform (QMAP) system: A multicenter study in Korea

Hye Young Wang, Kwangjin Ahn, Young Uh, Hyeyoung Lee, Seoyong Kim, Yunhee Chang, Chulhun L. Chang, Tae Sun Shim

Research output: Contribution to journalArticle

Abstract

Rapid and accurate detection of rifampin-resistant Mycobacterium tuberculosis (MTB) is of primary importance for infection control and selection of anti-tuberculosis drugs. The aim of this study was to evaluate the usefulness of a newly developed multiplexed, bead-based bioassay (Quantamatrix Multiplexed Assay Platform, QMAP) for the direct detection of rifampin-resistant MTB in respiratory specimens. A total of 400 respiratory specimens collected from patients with clinically suspected tuberculosis or non-tuberculous mycobacteria (NTM) infections were tested with the culture-based conventional Mycobacterium species identification and QMAP system. Among 400 specimens, 154 samples were evaluated using phenotypic anti-tuberculosis drug susceptibility test (DST) and the QMAP system for the detection of rifampin resistance. Detection agreement rate between the culture-based conventional identification and QMAP system for MTB and NTM according to acid-fast bacillus smear positivity was as follows: 97.0% (131/135) and 93.6% (88/94) in 229 smear-positive samples and 69.4% (25/36) and 73.0% (65/89) in 171 smear-negative samples. Based on culture as the gold standard, the overall sensitivity and specificity of the QMAP system for Mycobacterium identification were 87.3 and 97.8%, respectively. The categorical agreement rate between phenotypic DST and QMAP system for rifampin was as follows: complete agreement, 92.9% (143/154); very major error, 0%; and major error, 0.6% (1/154). The overall sensitivity of the QMAP system for the detection of rifampin resistance was 97.1% (34/35). The QMAP system is a useful screening method for the early diagnosis of tuberculosis and selection of anti-tuberculosis drug, as it may detect rifampin-resistant MTB directly from respiratory specimens.

Original languageEnglish
Article number1804
JournalFrontiers in Microbiology
Volume9
Issue numberAUG
DOIs
Publication statusPublished - 2018 Aug 17

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Rifampin
Korea
Mycobacterium tuberculosis
Multicenter Studies
Tuberculosis
Mycobacterium
Pharmaceutical Preparations
Mycobacterium Infections
Infection Control
Biological Assay
Bacillus
Early Diagnosis
Sensitivity and Specificity
Acids

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

Cite this

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title = "Direct detection of rifampin-resistant mycobacterium tuberculosis in respiratory specimens using Quantamatrix Multiplexed Assay Platform (QMAP) system: A multicenter study in Korea",
abstract = "Rapid and accurate detection of rifampin-resistant Mycobacterium tuberculosis (MTB) is of primary importance for infection control and selection of anti-tuberculosis drugs. The aim of this study was to evaluate the usefulness of a newly developed multiplexed, bead-based bioassay (Quantamatrix Multiplexed Assay Platform, QMAP) for the direct detection of rifampin-resistant MTB in respiratory specimens. A total of 400 respiratory specimens collected from patients with clinically suspected tuberculosis or non-tuberculous mycobacteria (NTM) infections were tested with the culture-based conventional Mycobacterium species identification and QMAP system. Among 400 specimens, 154 samples were evaluated using phenotypic anti-tuberculosis drug susceptibility test (DST) and the QMAP system for the detection of rifampin resistance. Detection agreement rate between the culture-based conventional identification and QMAP system for MTB and NTM according to acid-fast bacillus smear positivity was as follows: 97.0{\%} (131/135) and 93.6{\%} (88/94) in 229 smear-positive samples and 69.4{\%} (25/36) and 73.0{\%} (65/89) in 171 smear-negative samples. Based on culture as the gold standard, the overall sensitivity and specificity of the QMAP system for Mycobacterium identification were 87.3 and 97.8{\%}, respectively. The categorical agreement rate between phenotypic DST and QMAP system for rifampin was as follows: complete agreement, 92.9{\%} (143/154); very major error, 0{\%}; and major error, 0.6{\%} (1/154). The overall sensitivity of the QMAP system for the detection of rifampin resistance was 97.1{\%} (34/35). The QMAP system is a useful screening method for the early diagnosis of tuberculosis and selection of anti-tuberculosis drug, as it may detect rifampin-resistant MTB directly from respiratory specimens.",
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Direct detection of rifampin-resistant mycobacterium tuberculosis in respiratory specimens using Quantamatrix Multiplexed Assay Platform (QMAP) system : A multicenter study in Korea. / Wang, Hye Young; Ahn, Kwangjin; Uh, Young; Lee, Hyeyoung; Kim, Seoyong; Chang, Yunhee; Chang, Chulhun L.; Shim, Tae Sun.

In: Frontiers in Microbiology, Vol. 9, No. AUG, 1804, 17.08.2018.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Direct detection of rifampin-resistant mycobacterium tuberculosis in respiratory specimens using Quantamatrix Multiplexed Assay Platform (QMAP) system

T2 - A multicenter study in Korea

AU - Wang, Hye Young

AU - Ahn, Kwangjin

AU - Uh, Young

AU - Lee, Hyeyoung

AU - Kim, Seoyong

AU - Chang, Yunhee

AU - Chang, Chulhun L.

AU - Shim, Tae Sun

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