Direct force measurement of the interaction between liposome and the C2A domain of synaptotagmin I using atomic force microscopy

Jong Ho Park, Eun Young Kwon, Hyo Il Jung, Dae Eun Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The binding force between a liposome and the C2A domain of synaptotagmin I was determined by an atomic force microscopy (AFM). Liposomes were immobilized on the surface of the L1 sensor chip and the C2A domains, which recognize phosphatidylserine, were chemically conjugated onto a gold-coated cantilever tip. The average interaction force between the C2A domain and the liposome was 306 (±57) pN while the force between untreated cantilever and the liposome was 58 (±16) pN. This work helps understand the physicochemical interactions between proteins and lipid vesicles for the design of high affinity protein probes against the apoptotic cell surface.

Original languageEnglish
Pages (from-to)505-509
Number of pages5
JournalBiotechnology Letters
Volume28
Issue number7
DOIs
Publication statusPublished - 2006 Apr 1

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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