Direct force measurement of the interaction between liposome and the C2A domain of synaptotagmin I using atomic force microscopy

Jong Ho Park, Eun Young Kwon, Hyo Il Jung, Dae Eun Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The binding force between a liposome and the C2A domain of synaptotagmin I was determined by an atomic force microscopy (AFM). Liposomes were immobilized on the surface of the L1 sensor chip and the C2A domains, which recognize phosphatidylserine, were chemically conjugated onto a gold-coated cantilever tip. The average interaction force between the C2A domain and the liposome was 306 (±57) pN while the force between untreated cantilever and the liposome was 58 (±16) pN. This work helps understand the physicochemical interactions between proteins and lipid vesicles for the design of high affinity protein probes against the apoptotic cell surface.

Original languageEnglish
Pages (from-to)505-509
Number of pages5
JournalBiotechnology Letters
Volume28
Issue number7
DOIs
Publication statusPublished - 2006 Apr 1

Fingerprint

Synaptotagmin I
Liposomes
Force measurement
Atomic Force Microscopy
Atomic force microscopy
Proteins
Phosphatidylserines
Gold
Lipids
Cells
Sensors

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

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Direct force measurement of the interaction between liposome and the C2A domain of synaptotagmin I using atomic force microscopy. / Park, Jong Ho; Kwon, Eun Young; Jung, Hyo Il; Kim, Dae Eun.

In: Biotechnology Letters, Vol. 28, No. 7, 01.04.2006, p. 505-509.

Research output: Contribution to journalArticle

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