Direct identification of urinary tract pathogens from urine samples using the Vitek MS system based on matrix-assisted laser desorption ionization-time of flight mass spectrometry

Yeongsic Kim, Kang Gyun Park, Kyungwon Lee, Yeon Joon Park

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27 Citations (Scopus)

Abstract

Background: We evaluated the coincidence rate between Vitek MS system (bioMérieux, France) and Vitek 2 in identifying uropathogens directly from urine specimens. Methods: Urine specimens submitted to our microbiology laboratory between July and September 2013 for Gram staining and bacterial culture were analyzed. Bacterial identification was performed by using the conventional method. Urine specimens showing a single morphotype by Gram staining were processed by culturing and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Of 2,370 urine specimens, 251 showed a single morphotype on Gram staining, and among them, 202 were available for MALDI-TOF MS. Results: In these 202 specimens, colony growth was observed in 189 specimens, and 145 specimens had significant growth of single-colony morphotype in culture. One hundred and ten (75.9%) of them had colony counts of =105 colony-forming units (CFU)/mL and included 71 enteric gram-negative bacteria (GNB), 5 glucose-non-fermenting GNB, 9 gram-positive cocci (GPC), and 25 yeasts. Furthermore, 70 (98.6%), 3 (60.0%), 4 (44.4%), and 5 (20.0%), respectively, of these were correctly identified by Vitek MS. Thirty-one specimens (21.4%; 11 GNB, 7 GPC, 12 yeasts, and 1 gram-positive bacillus) had colony counts of 104-104 CFU/mL. Four specimens (2.8%) yielded colony counts of 103-104 CFU/mL. Conclusions: Vitek MS showed high rate of accuracy for the identification of GNB in urine specimens (≥105 CFU/mL). This could become a rapid and accurate diagnostic method for urinary tract infection caused by GNB. However, for the identification of GPC and yeasts, further studies on appropriate pre-treatment are warranted.

Original languageEnglish
Pages (from-to)416-422
Number of pages7
JournalAnnals of laboratory medicine
Volume35
Issue number4
DOIs
Publication statusPublished - 2015 Jan 1

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Pathogens
Gram-Negative Bacteria
Urinary Tract
Ionization
Mass spectrometry
Desorption
Mass Spectrometry
Bacteria
Lasers
Gram-Positive Cocci
Urine
Stem Cells
Yeast
Yeasts
Staining and Labeling
Microbiology
Bacilli
Enterobacteriaceae
Growth
Urinary Tract Infections

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

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title = "Direct identification of urinary tract pathogens from urine samples using the Vitek MS system based on matrix-assisted laser desorption ionization-time of flight mass spectrometry",
abstract = "Background: We evaluated the coincidence rate between Vitek MS system (bioM{\'e}rieux, France) and Vitek 2 in identifying uropathogens directly from urine specimens. Methods: Urine specimens submitted to our microbiology laboratory between July and September 2013 for Gram staining and bacterial culture were analyzed. Bacterial identification was performed by using the conventional method. Urine specimens showing a single morphotype by Gram staining were processed by culturing and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Of 2,370 urine specimens, 251 showed a single morphotype on Gram staining, and among them, 202 were available for MALDI-TOF MS. Results: In these 202 specimens, colony growth was observed in 189 specimens, and 145 specimens had significant growth of single-colony morphotype in culture. One hundred and ten (75.9{\%}) of them had colony counts of =105 colony-forming units (CFU)/mL and included 71 enteric gram-negative bacteria (GNB), 5 glucose-non-fermenting GNB, 9 gram-positive cocci (GPC), and 25 yeasts. Furthermore, 70 (98.6{\%}), 3 (60.0{\%}), 4 (44.4{\%}), and 5 (20.0{\%}), respectively, of these were correctly identified by Vitek MS. Thirty-one specimens (21.4{\%}; 11 GNB, 7 GPC, 12 yeasts, and 1 gram-positive bacillus) had colony counts of 104-104 CFU/mL. Four specimens (2.8{\%}) yielded colony counts of 103-104 CFU/mL. Conclusions: Vitek MS showed high rate of accuracy for the identification of GNB in urine specimens (≥105 CFU/mL). This could become a rapid and accurate diagnostic method for urinary tract infection caused by GNB. However, for the identification of GPC and yeasts, further studies on appropriate pre-treatment are warranted.",
author = "Yeongsic Kim and Park, {Kang Gyun} and Kyungwon Lee and Park, {Yeon Joon}",
year = "2015",
month = "1",
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doi = "10.3343/alm.2015.35.4.416",
language = "English",
volume = "35",
pages = "416--422",
journal = "Annals of Laboratory Medicine",
issn = "2234-3806",
publisher = "Seoul National University",
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T1 - Direct identification of urinary tract pathogens from urine samples using the Vitek MS system based on matrix-assisted laser desorption ionization-time of flight mass spectrometry

AU - Kim, Yeongsic

AU - Park, Kang Gyun

AU - Lee, Kyungwon

AU - Park, Yeon Joon

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Background: We evaluated the coincidence rate between Vitek MS system (bioMérieux, France) and Vitek 2 in identifying uropathogens directly from urine specimens. Methods: Urine specimens submitted to our microbiology laboratory between July and September 2013 for Gram staining and bacterial culture were analyzed. Bacterial identification was performed by using the conventional method. Urine specimens showing a single morphotype by Gram staining were processed by culturing and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Of 2,370 urine specimens, 251 showed a single morphotype on Gram staining, and among them, 202 were available for MALDI-TOF MS. Results: In these 202 specimens, colony growth was observed in 189 specimens, and 145 specimens had significant growth of single-colony morphotype in culture. One hundred and ten (75.9%) of them had colony counts of =105 colony-forming units (CFU)/mL and included 71 enteric gram-negative bacteria (GNB), 5 glucose-non-fermenting GNB, 9 gram-positive cocci (GPC), and 25 yeasts. Furthermore, 70 (98.6%), 3 (60.0%), 4 (44.4%), and 5 (20.0%), respectively, of these were correctly identified by Vitek MS. Thirty-one specimens (21.4%; 11 GNB, 7 GPC, 12 yeasts, and 1 gram-positive bacillus) had colony counts of 104-104 CFU/mL. Four specimens (2.8%) yielded colony counts of 103-104 CFU/mL. Conclusions: Vitek MS showed high rate of accuracy for the identification of GNB in urine specimens (≥105 CFU/mL). This could become a rapid and accurate diagnostic method for urinary tract infection caused by GNB. However, for the identification of GPC and yeasts, further studies on appropriate pre-treatment are warranted.

AB - Background: We evaluated the coincidence rate between Vitek MS system (bioMérieux, France) and Vitek 2 in identifying uropathogens directly from urine specimens. Methods: Urine specimens submitted to our microbiology laboratory between July and September 2013 for Gram staining and bacterial culture were analyzed. Bacterial identification was performed by using the conventional method. Urine specimens showing a single morphotype by Gram staining were processed by culturing and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Of 2,370 urine specimens, 251 showed a single morphotype on Gram staining, and among them, 202 were available for MALDI-TOF MS. Results: In these 202 specimens, colony growth was observed in 189 specimens, and 145 specimens had significant growth of single-colony morphotype in culture. One hundred and ten (75.9%) of them had colony counts of =105 colony-forming units (CFU)/mL and included 71 enteric gram-negative bacteria (GNB), 5 glucose-non-fermenting GNB, 9 gram-positive cocci (GPC), and 25 yeasts. Furthermore, 70 (98.6%), 3 (60.0%), 4 (44.4%), and 5 (20.0%), respectively, of these were correctly identified by Vitek MS. Thirty-one specimens (21.4%; 11 GNB, 7 GPC, 12 yeasts, and 1 gram-positive bacillus) had colony counts of 104-104 CFU/mL. Four specimens (2.8%) yielded colony counts of 103-104 CFU/mL. Conclusions: Vitek MS showed high rate of accuracy for the identification of GNB in urine specimens (≥105 CFU/mL). This could become a rapid and accurate diagnostic method for urinary tract infection caused by GNB. However, for the identification of GPC and yeasts, further studies on appropriate pre-treatment are warranted.

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U2 - 10.3343/alm.2015.35.4.416

DO - 10.3343/alm.2015.35.4.416

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JO - Annals of Laboratory Medicine

JF - Annals of Laboratory Medicine

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