Abstract
Blood hemoglobin is an important diagnostic parameter in measuring overall health. The hemoglobin molecule and the iron it contains absorb light energy, leading to thermal changes. This paper presents a new method for determining the hemoglobin concentration of erythrocytes by measuring temperature increases of the heme group when cells are heated by a 532 nm wavelength laser. The advantages of our method are that it determines the hemoglobin content of an entire blood sample without chemical treatments and requires only a small amount of blood (less than 10 μL). A micro scaled platinum resistance temperature detector (Pt RTD) was fabricated using a microelectromechanical system (MEMS) technique that directly measures the temperature changes. The platinum RTD's resistance at 0 °C is 275.32 Ω. For the specific heating of erythrocytes, we used a 0.03 to 9.6 W cm-2 power tunable diode pumped solid state (DPSS) continuous wave (CW) laser module with a wavelength of 532 nm. When heating human erythrocytes, leukocytes, plasma, and reference solutions, only the temperature of the erythrocytes significantly increased, indicating that our measurement technique can be used to determine hemoglobin concentration. The hemoglobin concentrations for the samples we used were 0.34, 0.67, 1.35, 2.7, 5.4, 8.1, 10.8, 13.5, 16.2, 18.9 and 21.6 g dL-1. The temperatures measured for each sample were 31.17 ± 1.98, 36.34 ± 3.76, 42.70 ± 4.38, 48.39 ± 6.47, 63.73 ± 3.34, 79.09 ± 9.60, 84.86 ± 1.99, 87.54 ± 9.84, 91.90 ± 5.27, 90.00 ± 3.24 and 95.79 ± 2.66 °C at a 9.6 W cm -2 output power of the 532 nm laser at 23 °C. We also provide a theoretical analysis of the temperature increases and investigate their major heat source.
| Original language | English |
|---|---|
| Pages (from-to) | 2365-2371 |
| Number of pages | 7 |
| Journal | Analyst |
| Volume | 135 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2010 Sep 1 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Biochemistry
- Environmental Chemistry
- Spectroscopy
- Electrochemistry
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Direct measurement of the in vitro hemoglobin content of erythrocytes using the photo-thermal effect of the heme group. / Kwak, Bong Seop; Kim, Beom Seok; Song, Suk Heung; Kim, Hyun Ok; Cho, Hyung Hee; Jung, Hyo Il.
In: Analyst, Vol. 135, No. 9, 01.09.2010, p. 2365-2371.Research output: Contribution to journal › Article
TY - JOUR
T1 - Direct measurement of the in vitro hemoglobin content of erythrocytes using the photo-thermal effect of the heme group
AU - Kwak, Bong Seop
AU - Kim, Beom Seok
AU - Song, Suk Heung
AU - Kim, Hyun Ok
AU - Cho, Hyung Hee
AU - Jung, Hyo Il
PY - 2010/9/1
Y1 - 2010/9/1
N2 - Blood hemoglobin is an important diagnostic parameter in measuring overall health. The hemoglobin molecule and the iron it contains absorb light energy, leading to thermal changes. This paper presents a new method for determining the hemoglobin concentration of erythrocytes by measuring temperature increases of the heme group when cells are heated by a 532 nm wavelength laser. The advantages of our method are that it determines the hemoglobin content of an entire blood sample without chemical treatments and requires only a small amount of blood (less than 10 μL). A micro scaled platinum resistance temperature detector (Pt RTD) was fabricated using a microelectromechanical system (MEMS) technique that directly measures the temperature changes. The platinum RTD's resistance at 0 °C is 275.32 Ω. For the specific heating of erythrocytes, we used a 0.03 to 9.6 W cm-2 power tunable diode pumped solid state (DPSS) continuous wave (CW) laser module with a wavelength of 532 nm. When heating human erythrocytes, leukocytes, plasma, and reference solutions, only the temperature of the erythrocytes significantly increased, indicating that our measurement technique can be used to determine hemoglobin concentration. The hemoglobin concentrations for the samples we used were 0.34, 0.67, 1.35, 2.7, 5.4, 8.1, 10.8, 13.5, 16.2, 18.9 and 21.6 g dL-1. The temperatures measured for each sample were 31.17 ± 1.98, 36.34 ± 3.76, 42.70 ± 4.38, 48.39 ± 6.47, 63.73 ± 3.34, 79.09 ± 9.60, 84.86 ± 1.99, 87.54 ± 9.84, 91.90 ± 5.27, 90.00 ± 3.24 and 95.79 ± 2.66 °C at a 9.6 W cm -2 output power of the 532 nm laser at 23 °C. We also provide a theoretical analysis of the temperature increases and investigate their major heat source.
AB - Blood hemoglobin is an important diagnostic parameter in measuring overall health. The hemoglobin molecule and the iron it contains absorb light energy, leading to thermal changes. This paper presents a new method for determining the hemoglobin concentration of erythrocytes by measuring temperature increases of the heme group when cells are heated by a 532 nm wavelength laser. The advantages of our method are that it determines the hemoglobin content of an entire blood sample without chemical treatments and requires only a small amount of blood (less than 10 μL). A micro scaled platinum resistance temperature detector (Pt RTD) was fabricated using a microelectromechanical system (MEMS) technique that directly measures the temperature changes. The platinum RTD's resistance at 0 °C is 275.32 Ω. For the specific heating of erythrocytes, we used a 0.03 to 9.6 W cm-2 power tunable diode pumped solid state (DPSS) continuous wave (CW) laser module with a wavelength of 532 nm. When heating human erythrocytes, leukocytes, plasma, and reference solutions, only the temperature of the erythrocytes significantly increased, indicating that our measurement technique can be used to determine hemoglobin concentration. The hemoglobin concentrations for the samples we used were 0.34, 0.67, 1.35, 2.7, 5.4, 8.1, 10.8, 13.5, 16.2, 18.9 and 21.6 g dL-1. The temperatures measured for each sample were 31.17 ± 1.98, 36.34 ± 3.76, 42.70 ± 4.38, 48.39 ± 6.47, 63.73 ± 3.34, 79.09 ± 9.60, 84.86 ± 1.99, 87.54 ± 9.84, 91.90 ± 5.27, 90.00 ± 3.24 and 95.79 ± 2.66 °C at a 9.6 W cm -2 output power of the 532 nm laser at 23 °C. We also provide a theoretical analysis of the temperature increases and investigate their major heat source.
UR - http://www.scopus.com/inward/record.url?scp=77955790538&partnerID=8YFLogxK
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U2 - 10.1039/c0an00235f
DO - 10.1039/c0an00235f
M3 - Article
C2 - 20648393
AN - SCOPUS:77955790538
VL - 135
SP - 2365
EP - 2371
JO - The Analyst
JF - The Analyst
SN - 0003-2654
IS - 9
ER -