Direct photo-patterning on anthracene containing polymer for guiding stem cell adhesion

Jungmok You; June Seok Heo; Hyun Ok Kim; Eunkyoung Kim

doi:10.1186/s40824-016-0072-4

Direct photo-patterning on anthracene containing polymer for guiding stem cell adhesion

Jungmok You, June Seok Heo, Hyun Ok Kim, Eunkyoung Kim

Department of Chemical and Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Background: Various micropatterned surfaces capable of guiding the selective adhesion of biomolecules such as proteins and cells are of great interests in biosensor, diagnostics, drug screening, and tissue engineering. In this study, we described a simple photo-patterning method to prepare micro-patterned films for stem cell patterning using anthracene containing polymers (PMAn). This micro patterned polymer film was prepared by the facile photo-reaction of anthracene units in polymer backbone structure. Results: The UV irradiation of PMAn through a photomask resulted in the quenching of fluorescent intensity as well as the changes in surface wettability from hydrophobic to hydrophilic surface. As a result, UV exposed regions of PMAn film show lower fluorescent intensity as well as higher proliferation rate of mesenchymal stem cells (MSCs) than unexposed region of PMAn film. Furthermore, the selective MSC attachment was clearly observed in the UV exposed regions of PMAn film. Conclusion: We developed a simple cell patterning method with a fluorescent, biocompatible, and patternable polymer film containing anthracene units. This method provides a facile stem cell patterning method and could be extended to various patterning of biomaterials without labor-intensive preparation and no pre-treatment for complex interactions of cell-microenvironment.

Original language	English
Article number	26
Journal	Biomaterials Research
Volume	20
Issue number	1
DOIs	https://doi.org/10.1186/s40824-016-0072-4
Publication status	Published - 2016

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRT) grant funded by the Korea government (MSIP) and a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health Welfare, Republic Korea. This work was supported by the National Research Foundation of Korea (NRT) grant funded by the Korea government (MSIP) (No. 2015R1C1A1A01054258) and a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health and Welfare, Republic Korea (No. HI15C0942).

Publisher Copyright:
© 2016 The Author(s).

All Science Journal Classification (ASJC) codes

Ceramics and Composites
Medicine (miscellaneous)
Biomaterials
Biomedical Engineering

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10.1186/s40824-016-0072-4

Cite this

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title = "Direct photo-patterning on anthracene containing polymer for guiding stem cell adhesion",

abstract = "Background: Various micropatterned surfaces capable of guiding the selective adhesion of biomolecules such as proteins and cells are of great interests in biosensor, diagnostics, drug screening, and tissue engineering. In this study, we described a simple photo-patterning method to prepare micro-patterned films for stem cell patterning using anthracene containing polymers (PMAn). This micro patterned polymer film was prepared by the facile photo-reaction of anthracene units in polymer backbone structure. Results: The UV irradiation of PMAn through a photomask resulted in the quenching of fluorescent intensity as well as the changes in surface wettability from hydrophobic to hydrophilic surface. As a result, UV exposed regions of PMAn film show lower fluorescent intensity as well as higher proliferation rate of mesenchymal stem cells (MSCs) than unexposed region of PMAn film. Furthermore, the selective MSC attachment was clearly observed in the UV exposed regions of PMAn film. Conclusion: We developed a simple cell patterning method with a fluorescent, biocompatible, and patternable polymer film containing anthracene units. This method provides a facile stem cell patterning method and could be extended to various patterning of biomaterials without labor-intensive preparation and no pre-treatment for complex interactions of cell-microenvironment.",

author = "Jungmok You and Heo, {June Seok} and Kim, {Hyun Ok} and Eunkyoung Kim",

note = "Funding Information: This work was supported by the National Research Foundation of Korea (NRT) grant funded by the Korea government (MSIP) and a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health Welfare, Republic Korea. This work was supported by the National Research Foundation of Korea (NRT) grant funded by the Korea government (MSIP) (No. 2015R1C1A1A01054258) and a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health and Welfare, Republic Korea (No. HI15C0942). Publisher Copyright: {\textcopyright} 2016 The Author(s).",

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AU - Heo, June Seok

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AU - Kim, Eunkyoung

N1 - Funding Information: This work was supported by the National Research Foundation of Korea (NRT) grant funded by the Korea government (MSIP) and a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health Welfare, Republic Korea. This work was supported by the National Research Foundation of Korea (NRT) grant funded by the Korea government (MSIP) (No. 2015R1C1A1A01054258) and a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health and Welfare, Republic Korea (No. HI15C0942). Publisher Copyright: © 2016 The Author(s).

PY - 2016

Y1 - 2016

N2 - Background: Various micropatterned surfaces capable of guiding the selective adhesion of biomolecules such as proteins and cells are of great interests in biosensor, diagnostics, drug screening, and tissue engineering. In this study, we described a simple photo-patterning method to prepare micro-patterned films for stem cell patterning using anthracene containing polymers (PMAn). This micro patterned polymer film was prepared by the facile photo-reaction of anthracene units in polymer backbone structure. Results: The UV irradiation of PMAn through a photomask resulted in the quenching of fluorescent intensity as well as the changes in surface wettability from hydrophobic to hydrophilic surface. As a result, UV exposed regions of PMAn film show lower fluorescent intensity as well as higher proliferation rate of mesenchymal stem cells (MSCs) than unexposed region of PMAn film. Furthermore, the selective MSC attachment was clearly observed in the UV exposed regions of PMAn film. Conclusion: We developed a simple cell patterning method with a fluorescent, biocompatible, and patternable polymer film containing anthracene units. This method provides a facile stem cell patterning method and could be extended to various patterning of biomaterials without labor-intensive preparation and no pre-treatment for complex interactions of cell-microenvironment.

AB - Background: Various micropatterned surfaces capable of guiding the selective adhesion of biomolecules such as proteins and cells are of great interests in biosensor, diagnostics, drug screening, and tissue engineering. In this study, we described a simple photo-patterning method to prepare micro-patterned films for stem cell patterning using anthracene containing polymers (PMAn). This micro patterned polymer film was prepared by the facile photo-reaction of anthracene units in polymer backbone structure. Results: The UV irradiation of PMAn through a photomask resulted in the quenching of fluorescent intensity as well as the changes in surface wettability from hydrophobic to hydrophilic surface. As a result, UV exposed regions of PMAn film show lower fluorescent intensity as well as higher proliferation rate of mesenchymal stem cells (MSCs) than unexposed region of PMAn film. Furthermore, the selective MSC attachment was clearly observed in the UV exposed regions of PMAn film. Conclusion: We developed a simple cell patterning method with a fluorescent, biocompatible, and patternable polymer film containing anthracene units. This method provides a facile stem cell patterning method and could be extended to various patterning of biomaterials without labor-intensive preparation and no pre-treatment for complex interactions of cell-microenvironment.

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Direct photo-patterning on anthracene containing polymer for guiding stem cell adhesion

Abstract

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