Direct photo-patterning on anthracene containing polymer for guiding stem cell adhesion

Jungmok You, June Seok Heo, Hyun Ok Kim, Eunkyoung Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: Various micropatterned surfaces capable of guiding the selective adhesion of biomolecules such as proteins and cells are of great interests in biosensor, diagnostics, drug screening, and tissue engineering. In this study, we described a simple photo-patterning method to prepare micro-patterned films for stem cell patterning using anthracene containing polymers (PMAn). This micro patterned polymer film was prepared by the facile photo-reaction of anthracene units in polymer backbone structure. Results: The UV irradiation of PMAn through a photomask resulted in the quenching of fluorescent intensity as well as the changes in surface wettability from hydrophobic to hydrophilic surface. As a result, UV exposed regions of PMAn film show lower fluorescent intensity as well as higher proliferation rate of mesenchymal stem cells (MSCs) than unexposed region of PMAn film. Furthermore, the selective MSC attachment was clearly observed in the UV exposed regions of PMAn film. Conclusion: We developed a simple cell patterning method with a fluorescent, biocompatible, and patternable polymer film containing anthracene units. This method provides a facile stem cell patterning method and could be extended to various patterning of biomaterials without labor-intensive preparation and no pre-treatment for complex interactions of cell-microenvironment.

Original languageEnglish
Article number26
JournalBiomaterials Research
Volume20
Issue number1
DOIs
Publication statusPublished - 2016 Jan 1

Fingerprint

Anthracene
Cell adhesion
Stem cells
Cell Adhesion
Polymers
Stem Cells
Mesenchymal Stromal Cells
Polymer films
Wettability
Cellular Microenvironment
Photomasks
Preclinical Drug Evaluations
Biocompatible Materials
Biosensing Techniques
Biomolecules
Tissue Engineering
Tissue engineering
Biosensors
Biomaterials
Wetting

All Science Journal Classification (ASJC) codes

  • Ceramics and Composites
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

Cite this

@article{933ccf6c0ea84a829736e598298f7c12,
title = "Direct photo-patterning on anthracene containing polymer for guiding stem cell adhesion",
abstract = "Background: Various micropatterned surfaces capable of guiding the selective adhesion of biomolecules such as proteins and cells are of great interests in biosensor, diagnostics, drug screening, and tissue engineering. In this study, we described a simple photo-patterning method to prepare micro-patterned films for stem cell patterning using anthracene containing polymers (PMAn). This micro patterned polymer film was prepared by the facile photo-reaction of anthracene units in polymer backbone structure. Results: The UV irradiation of PMAn through a photomask resulted in the quenching of fluorescent intensity as well as the changes in surface wettability from hydrophobic to hydrophilic surface. As a result, UV exposed regions of PMAn film show lower fluorescent intensity as well as higher proliferation rate of mesenchymal stem cells (MSCs) than unexposed region of PMAn film. Furthermore, the selective MSC attachment was clearly observed in the UV exposed regions of PMAn film. Conclusion: We developed a simple cell patterning method with a fluorescent, biocompatible, and patternable polymer film containing anthracene units. This method provides a facile stem cell patterning method and could be extended to various patterning of biomaterials without labor-intensive preparation and no pre-treatment for complex interactions of cell-microenvironment.",
author = "Jungmok You and Heo, {June Seok} and Kim, {Hyun Ok} and Eunkyoung Kim",
year = "2016",
month = "1",
day = "1",
doi = "10.1186/s40824-016-0072-4",
language = "English",
volume = "20",
journal = "Biomaterials Research",
issn = "2055-7124",
publisher = "John Wiley and Sons Inc.",
number = "1",

}

Direct photo-patterning on anthracene containing polymer for guiding stem cell adhesion. / You, Jungmok; Heo, June Seok; Kim, Hyun Ok; Kim, Eunkyoung.

In: Biomaterials Research, Vol. 20, No. 1, 26, 01.01.2016.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Direct photo-patterning on anthracene containing polymer for guiding stem cell adhesion

AU - You, Jungmok

AU - Heo, June Seok

AU - Kim, Hyun Ok

AU - Kim, Eunkyoung

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Background: Various micropatterned surfaces capable of guiding the selective adhesion of biomolecules such as proteins and cells are of great interests in biosensor, diagnostics, drug screening, and tissue engineering. In this study, we described a simple photo-patterning method to prepare micro-patterned films for stem cell patterning using anthracene containing polymers (PMAn). This micro patterned polymer film was prepared by the facile photo-reaction of anthracene units in polymer backbone structure. Results: The UV irradiation of PMAn through a photomask resulted in the quenching of fluorescent intensity as well as the changes in surface wettability from hydrophobic to hydrophilic surface. As a result, UV exposed regions of PMAn film show lower fluorescent intensity as well as higher proliferation rate of mesenchymal stem cells (MSCs) than unexposed region of PMAn film. Furthermore, the selective MSC attachment was clearly observed in the UV exposed regions of PMAn film. Conclusion: We developed a simple cell patterning method with a fluorescent, biocompatible, and patternable polymer film containing anthracene units. This method provides a facile stem cell patterning method and could be extended to various patterning of biomaterials without labor-intensive preparation and no pre-treatment for complex interactions of cell-microenvironment.

AB - Background: Various micropatterned surfaces capable of guiding the selective adhesion of biomolecules such as proteins and cells are of great interests in biosensor, diagnostics, drug screening, and tissue engineering. In this study, we described a simple photo-patterning method to prepare micro-patterned films for stem cell patterning using anthracene containing polymers (PMAn). This micro patterned polymer film was prepared by the facile photo-reaction of anthracene units in polymer backbone structure. Results: The UV irradiation of PMAn through a photomask resulted in the quenching of fluorescent intensity as well as the changes in surface wettability from hydrophobic to hydrophilic surface. As a result, UV exposed regions of PMAn film show lower fluorescent intensity as well as higher proliferation rate of mesenchymal stem cells (MSCs) than unexposed region of PMAn film. Furthermore, the selective MSC attachment was clearly observed in the UV exposed regions of PMAn film. Conclusion: We developed a simple cell patterning method with a fluorescent, biocompatible, and patternable polymer film containing anthracene units. This method provides a facile stem cell patterning method and could be extended to various patterning of biomaterials without labor-intensive preparation and no pre-treatment for complex interactions of cell-microenvironment.

UR - http://www.scopus.com/inward/record.url?scp=85030983187&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85030983187&partnerID=8YFLogxK

U2 - 10.1186/s40824-016-0072-4

DO - 10.1186/s40824-016-0072-4

M3 - Article

AN - SCOPUS:85030983187

VL - 20

JO - Biomaterials Research

JF - Biomaterials Research

SN - 2055-7124

IS - 1

M1 - 26

ER -