Discrimination of Scrophularia spp. according to geographic origin with HPLC-DAD combined with multivariate analysis

Seung Hyun Kim, Dae Hyun Kim, Jin Ho Park, Eun Jung Choi, Sunghyouk Park, Ki Yong Lee, Min Ji Jeon, Young Choong Kim, Sang Hyun Sung

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Discrimination of Scrophularia spp. according to the geographic origin was tried in the present study using HPLC-DAD combined with multivariate analysis techniques. Five active constituents, angoroside C, harpagoside, 8-O-(E-p-methoxycinnamoyl)harpagide, E-cinnamic acid and E-p-methoxycinnamic acid, in forty four Scrophularia samples were simultaneously determined using HPLC-DAD. A principal component analysis of the content measurements clustered the samples according to their geographic origins, Andong, Uisung and China. A partial least squares-discrimination analysis was subsequently developed for the effective classification of the samples. This model showed comparatively good prediction ability for samples from Andong or China. The proposed method shows an efficient strategy for quality control of Scrophularia spp.

Original languageEnglish
Pages (from-to)118-124
Number of pages7
JournalMicrochemical Journal
Volume94
Issue number2
DOIs
Publication statusPublished - 2010 Mar 1

Fingerprint

Principal component analysis
Quality control
cinnamic acid
Multivariate Analysis
angoroside C
4-methoxycinnamic acid
harpagide
harpagoside

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Spectroscopy

Cite this

Kim, Seung Hyun ; Kim, Dae Hyun ; Park, Jin Ho ; Choi, Eun Jung ; Park, Sunghyouk ; Lee, Ki Yong ; Jeon, Min Ji ; Kim, Young Choong ; Sung, Sang Hyun. / Discrimination of Scrophularia spp. according to geographic origin with HPLC-DAD combined with multivariate analysis. In: Microchemical Journal. 2010 ; Vol. 94, No. 2. pp. 118-124.
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Discrimination of Scrophularia spp. according to geographic origin with HPLC-DAD combined with multivariate analysis. / Kim, Seung Hyun; Kim, Dae Hyun; Park, Jin Ho; Choi, Eun Jung; Park, Sunghyouk; Lee, Ki Yong; Jeon, Min Ji; Kim, Young Choong; Sung, Sang Hyun.

In: Microchemical Journal, Vol. 94, No. 2, 01.03.2010, p. 118-124.

Research output: Contribution to journalArticle

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