Discrimination of Scrophulariae Radix according to geographical origin and determination of active constituents by near infrared spectroscopy (NIRS)

Dong Young Lee, Seung Hyun Kim, Young Choong Kim, Hyo Jin Kim, Sang Hyun Sung

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The potential of near infrared reflectance spectroscopy (NIR) was investigated for its ability to non-destructively discriminate the geographic origins of Scrophularia spp., Andong, Uisung and China. Application of principal component analysis to NIR spectra leads to a clear separation of Andong sample from the others. Moreover, the contents of two neuroprotective constituents of Scrophularia spp., 8- O-(E- p-methoxycinnamoyl)-harpagide (HG), and E- p-methoxycinnamic acid (MCA), were determined by HPLC-DAD. Partial least squares (PLS) regression of NIR spectra combined with these analytical reference data yield the development of calibration models for the contents of the two constituents. The correlation coefficients of prediction models for HG and MCA were >. 0.87. These outcomes indicated that the NIRS could be useful for the discrimination of Scrophularia spp.

Original languageEnglish
Pages (from-to)213-217
Number of pages5
JournalMicrochemical Journal
Volume99
Issue number2
DOIs
Publication statusPublished - 2011 Nov 1

Fingerprint

Near infrared spectroscopy
Spectroscopy
Infrared radiation
Principal component analysis
Calibration
Acids
harpagide

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Spectroscopy

Cite this

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title = "Discrimination of Scrophulariae Radix according to geographical origin and determination of active constituents by near infrared spectroscopy (NIRS)",
abstract = "The potential of near infrared reflectance spectroscopy (NIR) was investigated for its ability to non-destructively discriminate the geographic origins of Scrophularia spp., Andong, Uisung and China. Application of principal component analysis to NIR spectra leads to a clear separation of Andong sample from the others. Moreover, the contents of two neuroprotective constituents of Scrophularia spp., 8- O-(E- p-methoxycinnamoyl)-harpagide (HG), and E- p-methoxycinnamic acid (MCA), were determined by HPLC-DAD. Partial least squares (PLS) regression of NIR spectra combined with these analytical reference data yield the development of calibration models for the contents of the two constituents. The correlation coefficients of prediction models for HG and MCA were >. 0.87. These outcomes indicated that the NIRS could be useful for the discrimination of Scrophularia spp.",
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Discrimination of Scrophulariae Radix according to geographical origin and determination of active constituents by near infrared spectroscopy (NIRS). / Lee, Dong Young; Kim, Seung Hyun; Kim, Young Choong; Kim, Hyo Jin; Sung, Sang Hyun.

In: Microchemical Journal, Vol. 99, No. 2, 01.11.2011, p. 213-217.

Research output: Contribution to journalArticle

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