Abstract
The potential of near infrared reflectance spectroscopy (NIR) was investigated for its ability to non-destructively discriminate the geographic origins of Scrophularia spp., Andong, Uisung and China. Application of principal component analysis to NIR spectra leads to a clear separation of Andong sample from the others. Moreover, the contents of two neuroprotective constituents of Scrophularia spp., 8- O-(E- p-methoxycinnamoyl)-harpagide (HG), and E- p-methoxycinnamic acid (MCA), were determined by HPLC-DAD. Partial least squares (PLS) regression of NIR spectra combined with these analytical reference data yield the development of calibration models for the contents of the two constituents. The correlation coefficients of prediction models for HG and MCA were >. 0.87. These outcomes indicated that the NIRS could be useful for the discrimination of Scrophularia spp.
| Original language | English |
|---|---|
| Pages (from-to) | 213-217 |
| Number of pages | 5 |
| Journal | Microchemical Journal |
| Volume | 99 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2011 Nov 1 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Spectroscopy
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Discrimination of Scrophulariae Radix according to geographical origin and determination of active constituents by near infrared spectroscopy (NIRS). / Lee, Dong Young; Kim, Seung Hyun; Kim, Young Choong; Kim, Hyo Jin; Sung, Sang Hyun.
In: Microchemical Journal, Vol. 99, No. 2, 01.11.2011, p. 213-217.Research output: Contribution to journal › Article
TY - JOUR
T1 - Discrimination of Scrophulariae Radix according to geographical origin and determination of active constituents by near infrared spectroscopy (NIRS)
AU - Lee, Dong Young
AU - Kim, Seung Hyun
AU - Kim, Young Choong
AU - Kim, Hyo Jin
AU - Sung, Sang Hyun
PY - 2011/11/1
Y1 - 2011/11/1
N2 - The potential of near infrared reflectance spectroscopy (NIR) was investigated for its ability to non-destructively discriminate the geographic origins of Scrophularia spp., Andong, Uisung and China. Application of principal component analysis to NIR spectra leads to a clear separation of Andong sample from the others. Moreover, the contents of two neuroprotective constituents of Scrophularia spp., 8- O-(E- p-methoxycinnamoyl)-harpagide (HG), and E- p-methoxycinnamic acid (MCA), were determined by HPLC-DAD. Partial least squares (PLS) regression of NIR spectra combined with these analytical reference data yield the development of calibration models for the contents of the two constituents. The correlation coefficients of prediction models for HG and MCA were >. 0.87. These outcomes indicated that the NIRS could be useful for the discrimination of Scrophularia spp.
AB - The potential of near infrared reflectance spectroscopy (NIR) was investigated for its ability to non-destructively discriminate the geographic origins of Scrophularia spp., Andong, Uisung and China. Application of principal component analysis to NIR spectra leads to a clear separation of Andong sample from the others. Moreover, the contents of two neuroprotective constituents of Scrophularia spp., 8- O-(E- p-methoxycinnamoyl)-harpagide (HG), and E- p-methoxycinnamic acid (MCA), were determined by HPLC-DAD. Partial least squares (PLS) regression of NIR spectra combined with these analytical reference data yield the development of calibration models for the contents of the two constituents. The correlation coefficients of prediction models for HG and MCA were >. 0.87. These outcomes indicated that the NIRS could be useful for the discrimination of Scrophularia spp.
UR - http://www.scopus.com/inward/record.url?scp=80052353190&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80052353190&partnerID=8YFLogxK
U2 - 10.1016/j.microc.2011.05.008
DO - 10.1016/j.microc.2011.05.008
M3 - Article
VL - 99
SP - 213
EP - 217
JO - Microchemical Journal
JF - Microchemical Journal
SN - 0026-265X
IS - 2
ER -