Discrimination of Scrophulariae Radix according to geographical origin and determination of active constituents by near infrared spectroscopy (NIRS)

Dong Young Lee; Seung Hyun Kim; Young Choong Kim; Hyo Jin Kim; Sang Hyun Sung

doi:10.1016/j.microc.2011.05.008

Discrimination of Scrophulariae Radix according to geographical origin and determination of active constituents by near infrared spectroscopy (NIRS)

Dong Young Lee, Seung Hyun Kim, Young Choong Kim, Hyo Jin Kim, Sang Hyun Sung

Department of Pharmacy

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

The potential of near infrared reflectance spectroscopy (NIR) was investigated for its ability to non-destructively discriminate the geographic origins of Scrophularia spp., Andong, Uisung and China. Application of principal component analysis to NIR spectra leads to a clear separation of Andong sample from the others. Moreover, the contents of two neuroprotective constituents of Scrophularia spp., 8- O-(E- p-methoxycinnamoyl)-harpagide (HG), and E- p-methoxycinnamic acid (MCA), were determined by HPLC-DAD. Partial least squares (PLS) regression of NIR spectra combined with these analytical reference data yield the development of calibration models for the contents of the two constituents. The correlation coefficients of prediction models for HG and MCA were >. 0.87. These outcomes indicated that the NIRS could be useful for the discrimination of Scrophularia spp.

Original language	English
Pages (from-to)	213-217
Number of pages	5
Journal	Microchemical Journal
Volume	99
Issue number	2
DOIs	https://doi.org/10.1016/j.microc.2011.05.008
Publication status	Published - 2011 Nov

Bibliographical note

Funding Information:
This study was supported by the grant of Bruker Optics Korea . The Author specially thanks Mr. Michael Verst, CEO of Bruker Optics Korea

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Spectroscopy

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title = "Discrimination of Scrophulariae Radix according to geographical origin and determination of active constituents by near infrared spectroscopy (NIRS)",

abstract = "The potential of near infrared reflectance spectroscopy (NIR) was investigated for its ability to non-destructively discriminate the geographic origins of Scrophularia spp., Andong, Uisung and China. Application of principal component analysis to NIR spectra leads to a clear separation of Andong sample from the others. Moreover, the contents of two neuroprotective constituents of Scrophularia spp., 8- O-(E- p-methoxycinnamoyl)-harpagide (HG), and E- p-methoxycinnamic acid (MCA), were determined by HPLC-DAD. Partial least squares (PLS) regression of NIR spectra combined with these analytical reference data yield the development of calibration models for the contents of the two constituents. The correlation coefficients of prediction models for HG and MCA were >. 0.87. These outcomes indicated that the NIRS could be useful for the discrimination of Scrophularia spp.",

author = "Lee, {Dong Young} and Kim, {Seung Hyun} and Kim, {Young Choong} and Kim, {Hyo Jin} and Sung, {Sang Hyun}",

note = "Funding Information: This study was supported by the grant of Bruker Optics Korea . The Author specially thanks Mr. Michael Verst, CEO of Bruker Optics Korea",

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AU - Lee, Dong Young

AU - Kim, Seung Hyun

AU - Kim, Young Choong

AU - Kim, Hyo Jin

AU - Sung, Sang Hyun

N1 - Funding Information: This study was supported by the grant of Bruker Optics Korea . The Author specially thanks Mr. Michael Verst, CEO of Bruker Optics Korea

PY - 2011/11

Y1 - 2011/11

N2 - The potential of near infrared reflectance spectroscopy (NIR) was investigated for its ability to non-destructively discriminate the geographic origins of Scrophularia spp., Andong, Uisung and China. Application of principal component analysis to NIR spectra leads to a clear separation of Andong sample from the others. Moreover, the contents of two neuroprotective constituents of Scrophularia spp., 8- O-(E- p-methoxycinnamoyl)-harpagide (HG), and E- p-methoxycinnamic acid (MCA), were determined by HPLC-DAD. Partial least squares (PLS) regression of NIR spectra combined with these analytical reference data yield the development of calibration models for the contents of the two constituents. The correlation coefficients of prediction models for HG and MCA were >. 0.87. These outcomes indicated that the NIRS could be useful for the discrimination of Scrophularia spp.

AB - The potential of near infrared reflectance spectroscopy (NIR) was investigated for its ability to non-destructively discriminate the geographic origins of Scrophularia spp., Andong, Uisung and China. Application of principal component analysis to NIR spectra leads to a clear separation of Andong sample from the others. Moreover, the contents of two neuroprotective constituents of Scrophularia spp., 8- O-(E- p-methoxycinnamoyl)-harpagide (HG), and E- p-methoxycinnamic acid (MCA), were determined by HPLC-DAD. Partial least squares (PLS) regression of NIR spectra combined with these analytical reference data yield the development of calibration models for the contents of the two constituents. The correlation coefficients of prediction models for HG and MCA were >. 0.87. These outcomes indicated that the NIRS could be useful for the discrimination of Scrophularia spp.

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