Nanospeckle illumination microscopy (NanoSIM) based on disordered metallic nanocomposite island substrates is described. The nanoisland substrates can be fabricated without lithographic techniques. Azimuthal scanning illumination of nanoislands creates near-field distribution that excites an arbitrary number of basis images to produce super-resolution. Experimental studies of point-spread images using NanoSIM with 360 basis images obtained by azimuthal scanning find spatial resolution improved by more than three times with an achievable full-width-at-half-maximum at 81.1 nm on average. The approach is confirmed by imaging aggregated nanobeads and nanoscale distribution of gangliosides on the HeLa cell membrane.
Bibliographical noteFunding Information:
The authors are grateful for the support by the National Research Foundation (NRF) grants funded by the Korean Government (2019R1A4A1025958) and the Korea Medical Device Development Fund (Project Number: 202011D25). W.J.R. and J.‐S.S. acknowledge the support by the NRF (2019R1A6A1A03032869). The funding agencies had no role in the design or conduct of this study; collection, management, analysis, or interpretation of the data; preparation, review, or approval of the manuscript; or in the decision to submit the manuscript for publication.
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All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics