Disposable thick-film liposome immunosensor for theophylline using immunochromatographic matrix

Kang Shin Lee, Won Yong Lee, Je Kyun Park

Research output: Contribution to conferencePaper

Abstract

A novel electrochemical immunosensor based on liposome signal amplification and immunochromatography has been developed for the determination of theophylline, a therapeutic drug, as a model analyte. The immunosensor is composed of two major parts; a disposable screen-printed electrode and a nitrocellulose strip. The nitrocellulose strip contains a sample loading zone, an immobilized antibody competition zone, and a signal generation zone containing ferrocyanide-loaded liposomes in an ascending sequence. Upon loading liquid sample to the immunosensor, capillary action causes the theophylline and theophylline-melittin conjugate to migrate through an anti-theophylline antibody zone, where competition occurs. The unbound theophylline-melittin conjugates further migrate into the signal generation zone, where liposomes are disrupted to release the electroactive ferrocyanides which are amperometrically detected by the screen-printed electrode. The current produced is directly proportional to the amount of free theophylline in the sample. The lowest detection limit obtained with this immunosensor is 5 μg/ml.

Original languageEnglish
Pages195-198
Number of pages4
Publication statusPublished - 1997 Jan 1
EventProceedings of the 1997 International Conference on Solid-State Sensors and Actuators. Part 1 (of 2) - Chicago, IL, USA
Duration: 1997 Jun 161997 Jun 19

Other

OtherProceedings of the 1997 International Conference on Solid-State Sensors and Actuators. Part 1 (of 2)
CityChicago, IL, USA
Period97/6/1697/6/19

Fingerprint

Immunosensors
Liposomes
Thick films
Nitrocellulose
Antibodies
Electrodes
Amplification
Liquids

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Lee, K. S., Lee, W. Y., & Park, J. K. (1997). Disposable thick-film liposome immunosensor for theophylline using immunochromatographic matrix. 195-198. Paper presented at Proceedings of the 1997 International Conference on Solid-State Sensors and Actuators. Part 1 (of 2), Chicago, IL, USA, .
Lee, Kang Shin ; Lee, Won Yong ; Park, Je Kyun. / Disposable thick-film liposome immunosensor for theophylline using immunochromatographic matrix. Paper presented at Proceedings of the 1997 International Conference on Solid-State Sensors and Actuators. Part 1 (of 2), Chicago, IL, USA, .4 p.
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Lee, KS, Lee, WY & Park, JK 1997, 'Disposable thick-film liposome immunosensor for theophylline using immunochromatographic matrix', Paper presented at Proceedings of the 1997 International Conference on Solid-State Sensors and Actuators. Part 1 (of 2), Chicago, IL, USA, 97/6/16 - 97/6/19 pp. 195-198.

Disposable thick-film liposome immunosensor for theophylline using immunochromatographic matrix. / Lee, Kang Shin; Lee, Won Yong; Park, Je Kyun.

1997. 195-198 Paper presented at Proceedings of the 1997 International Conference on Solid-State Sensors and Actuators. Part 1 (of 2), Chicago, IL, USA, .

Research output: Contribution to conferencePaper

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N2 - A novel electrochemical immunosensor based on liposome signal amplification and immunochromatography has been developed for the determination of theophylline, a therapeutic drug, as a model analyte. The immunosensor is composed of two major parts; a disposable screen-printed electrode and a nitrocellulose strip. The nitrocellulose strip contains a sample loading zone, an immobilized antibody competition zone, and a signal generation zone containing ferrocyanide-loaded liposomes in an ascending sequence. Upon loading liquid sample to the immunosensor, capillary action causes the theophylline and theophylline-melittin conjugate to migrate through an anti-theophylline antibody zone, where competition occurs. The unbound theophylline-melittin conjugates further migrate into the signal generation zone, where liposomes are disrupted to release the electroactive ferrocyanides which are amperometrically detected by the screen-printed electrode. The current produced is directly proportional to the amount of free theophylline in the sample. The lowest detection limit obtained with this immunosensor is 5 μg/ml.

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Lee KS, Lee WY, Park JK. Disposable thick-film liposome immunosensor for theophylline using immunochromatographic matrix. 1997. Paper presented at Proceedings of the 1997 International Conference on Solid-State Sensors and Actuators. Part 1 (of 2), Chicago, IL, USA, .