Abstract
A novel electrochemical immunosensor based on liposome signal amplification and immunochromatography has been developed for the determination of theophylline, a therapeutic drug, as a model analyte. The immunosensor is composed of two major parts; a disposable screen-printed electrode and a nitrocellulose strip. The nitrocellulose strip contains a sample loading zone, an immobilized antibody competition zone, and a signal generation zone containing ferrocyanide-loaded liposomes in an ascending sequence. Upon loading liquid sample to the immunosensor, capillary action causes the theophylline and theophylline-melittin conjugate to migrate through an anti-theophylline antibody zone, where competition occurs. The unbound theophylline-melittin conjugates further migrate into the signal generation zone, where liposomes are disrupted to release the electroactive ferrocyanides which are amperometrically detected by the screen-printed electrode. The current produced is directly proportional to the amount of free theophylline in the sample. The lowest detection limit obtained with this immunosensor is 5 μg/ml.
| Original language | English |
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| Pages | 195-198 |
| Number of pages | 4 |
| Publication status | Published - 1997 |
| Event | Proceedings of the 1997 International Conference on Solid-State Sensors and Actuators. Part 1 (of 2) - Chicago, IL, USA Duration: 1997 Jun 16 → 1997 Jun 19 |
Other
| Other | Proceedings of the 1997 International Conference on Solid-State Sensors and Actuators. Part 1 (of 2) |
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| City | Chicago, IL, USA |
| Period | 97/6/16 → 97/6/19 |
All Science Journal Classification (ASJC) codes
- Engineering(all)