Disposable thick-film liposome immunosensor for theophylline using immunochromatographic matrix

Kang Shin Lee, Won Yong Lee, Je Kyun Park

Research output: Contribution to conferencePaper

Abstract

A novel electrochemical immunosensor based on liposome signal amplification and immunochromatography has been developed for the determination of theophylline, a therapeutic drug, as a model analyte. The immunosensor is composed of two major parts; a disposable screen-printed electrode and a nitrocellulose strip. The nitrocellulose strip contains a sample loading zone, an immobilized antibody competition zone, and a signal generation zone containing ferrocyanide-loaded liposomes in an ascending sequence. Upon loading liquid sample to the immunosensor, capillary action causes the theophylline and theophylline-melittin conjugate to migrate through an anti-theophylline antibody zone, where competition occurs. The unbound theophylline-melittin conjugates further migrate into the signal generation zone, where liposomes are disrupted to release the electroactive ferrocyanides which are amperometrically detected by the screen-printed electrode. The current produced is directly proportional to the amount of free theophylline in the sample. The lowest detection limit obtained with this immunosensor is 5 μg/ml.

Original languageEnglish
Pages195-198
Number of pages4
Publication statusPublished - 1997
EventProceedings of the 1997 International Conference on Solid-State Sensors and Actuators. Part 1 (of 2) - Chicago, IL, USA
Duration: 1997 Jun 161997 Jun 19

Other

OtherProceedings of the 1997 International Conference on Solid-State Sensors and Actuators. Part 1 (of 2)
CityChicago, IL, USA
Period97/6/1697/6/19

All Science Journal Classification (ASJC) codes

  • Engineering(all)

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    Lee, K. S., Lee, W. Y., & Park, J. K. (1997). Disposable thick-film liposome immunosensor for theophylline using immunochromatographic matrix. 195-198. Paper presented at Proceedings of the 1997 International Conference on Solid-State Sensors and Actuators. Part 1 (of 2), Chicago, IL, USA, .