Dissemination of 16S rRNA methylase-mediated highly amikacin-resistant isolates of Klebsiella pneumoniae and Acinetobacter baumannii in Korea

Hyukmin Lee; Dongeun Yong; Jong Hwa Yum; Kyoung Ho Roh; Kyungwon Lee; Kunikazu Yamane; Yoshichika Arakawa; Yunsop Chong

doi:10.1016/j.diagmicrobio.2006.05.002

Dissemination of 16S rRNA methylase-mediated highly amikacin-resistant isolates of Klebsiella pneumoniae and Acinetobacter baumannii in Korea

Hyukmin Lee, Dongeun Yong, Jong Hwa Yum, Kyoung Ho Roh, Kyungwon Lee, Kunikazu Yamane, Yoshichika Arakawa, Yunsop Chong

Department of Laboratory Medicine

Research output: Contribution to journal › Article › peer-review

72 Citations (Scopus)

Abstract

Novel 16S rRNA methylase-mediated high-level resistance to amikacin and arbekacin has been reported recently in clinical isolates of Gram-negative bacilli only from several countries. We tested amikacin- or arbekacin-nonsusceptible Gram-negative bacilli isolated in 2003 and 2005 at a tertiary-care hospital in Korea by polymerase chain reaction to detect 16S rRNA methylase genes. armA alleles were detected in 14 isolates of Klebsiella pneumoniae, 10 other species of Enterobacteriaceae, and 16 Acinetobacter baumannii, whereas the rmtB allele was detected in 1 K. pneumoniae isolate. The resistance 1st detected in 2003 persisted in 2005. 16S rRNA methylase-producing isolates were highly resistant to arbekacin and amikacin, and were mostly coresistant to levofloxacin. Most K. pneumoniae isolates also produced extended-spectrum β-lactamases and plasmid-mediated AmpC β-lactamases, and most A. baumannii isolates were nonsusceptible to carbapenems.

Original language	English
Pages (from-to)	305-312
Number of pages	8
Journal	Diagnostic Microbiology and Infectious Disease
Volume	56
Issue number	3
DOIs	https://doi.org/10.1016/j.diagmicrobio.2006.05.002
Publication status	Published - 2006 Nov

Bibliographical note

Funding Information:
This work was supported in part by the Brain Korea 21 Project for Medical Sciences, Yonsei University, Seoul, South Korea, in 2004. The authors thank Myungsook Kim for collecting the isolates and Younghee Seon and Chasoon Lee for technical assistance.

All Science Journal Classification (ASJC) codes

Microbiology (medical)
Infectious Diseases

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Lee, Hyukmin ; Yong, Dongeun ; Yum, Jong Hwa ; Roh, Kyoung Ho ; Lee, Kyungwon ; Yamane, Kunikazu ; Arakawa, Yoshichika ; Chong, Yunsop. / Dissemination of 16S rRNA methylase-mediated highly amikacin-resistant isolates of Klebsiella pneumoniae and Acinetobacter baumannii in Korea. In: Diagnostic Microbiology and Infectious Disease. 2006 ; Vol. 56, No. 3. pp. 305-312.

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title = "Dissemination of 16S rRNA methylase-mediated highly amikacin-resistant isolates of Klebsiella pneumoniae and Acinetobacter baumannii in Korea",

abstract = "Novel 16S rRNA methylase-mediated high-level resistance to amikacin and arbekacin has been reported recently in clinical isolates of Gram-negative bacilli only from several countries. We tested amikacin- or arbekacin-nonsusceptible Gram-negative bacilli isolated in 2003 and 2005 at a tertiary-care hospital in Korea by polymerase chain reaction to detect 16S rRNA methylase genes. armA alleles were detected in 14 isolates of Klebsiella pneumoniae, 10 other species of Enterobacteriaceae, and 16 Acinetobacter baumannii, whereas the rmtB allele was detected in 1 K. pneumoniae isolate. The resistance 1st detected in 2003 persisted in 2005. 16S rRNA methylase-producing isolates were highly resistant to arbekacin and amikacin, and were mostly coresistant to levofloxacin. Most K. pneumoniae isolates also produced extended-spectrum β-lactamases and plasmid-mediated AmpC β-lactamases, and most A. baumannii isolates were nonsusceptible to carbapenems.",

author = "Hyukmin Lee and Dongeun Yong and Yum, {Jong Hwa} and Roh, {Kyoung Ho} and Kyungwon Lee and Kunikazu Yamane and Yoshichika Arakawa and Yunsop Chong",

note = "Funding Information: This work was supported in part by the Brain Korea 21 Project for Medical Sciences, Yonsei University, Seoul, South Korea, in 2004. The authors thank Myungsook Kim for collecting the isolates and Younghee Seon and Chasoon Lee for technical assistance.",

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Dissemination of 16S rRNA methylase-mediated highly amikacin-resistant isolates of Klebsiella pneumoniae and Acinetobacter baumannii in Korea. / Lee, Hyukmin; Yong, Dongeun; Yum, Jong Hwa; Roh, Kyoung Ho; Lee, Kyungwon; Yamane, Kunikazu; Arakawa, Yoshichika; Chong, Yunsop.

In: Diagnostic Microbiology and Infectious Disease, Vol. 56, No. 3, 11.2006, p. 305-312.

Research output: Contribution to journal › Article › peer-review

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