Dissemination of 16S rRNA methylase-mediated highly amikacin-resistant isolates of Klebsiella pneumoniae and Acinetobacter baumannii in Korea

Hyukmin Lee, Dongeun Yong, Jong Hwa Yum, Kyoung Ho Roh, Kyungwon Lee, Kunikazu Yamane, Yoshichika Arakawa, Yunsop Chong

Research output: Contribution to journalArticle

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Abstract

Novel 16S rRNA methylase-mediated high-level resistance to amikacin and arbekacin has been reported recently in clinical isolates of Gram-negative bacilli only from several countries. We tested amikacin- or arbekacin-nonsusceptible Gram-negative bacilli isolated in 2003 and 2005 at a tertiary-care hospital in Korea by polymerase chain reaction to detect 16S rRNA methylase genes. armA alleles were detected in 14 isolates of Klebsiella pneumoniae, 10 other species of Enterobacteriaceae, and 16 Acinetobacter baumannii, whereas the rmtB allele was detected in 1 K. pneumoniae isolate. The resistance 1st detected in 2003 persisted in 2005. 16S rRNA methylase-producing isolates were highly resistant to arbekacin and amikacin, and were mostly coresistant to levofloxacin. Most K. pneumoniae isolates also produced extended-spectrum β-lactamases and plasmid-mediated AmpC β-lactamases, and most A. baumannii isolates were nonsusceptible to carbapenems.

Original languageEnglish
Pages (from-to)305-312
Number of pages8
JournalDiagnostic Microbiology and Infectious Disease
Volume56
Issue number3
DOIs
Publication statusPublished - 2006 Nov 1

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Acinetobacter baumannii
Amikacin
Klebsiella pneumoniae
Korea
Bacillus
Alleles
Levofloxacin
Carbapenems
Enterobacteriaceae
Tertiary Healthcare
rRNA Genes
Tertiary Care Centers
Plasmids
Polymerase Chain Reaction
habekacin
rRNA (adenosine-O-2'-)methyltransferase

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Lee, Hyukmin ; Yong, Dongeun ; Yum, Jong Hwa ; Roh, Kyoung Ho ; Lee, Kyungwon ; Yamane, Kunikazu ; Arakawa, Yoshichika ; Chong, Yunsop. / Dissemination of 16S rRNA methylase-mediated highly amikacin-resistant isolates of Klebsiella pneumoniae and Acinetobacter baumannii in Korea. In: Diagnostic Microbiology and Infectious Disease. 2006 ; Vol. 56, No. 3. pp. 305-312.
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Dissemination of 16S rRNA methylase-mediated highly amikacin-resistant isolates of Klebsiella pneumoniae and Acinetobacter baumannii in Korea. / Lee, Hyukmin; Yong, Dongeun; Yum, Jong Hwa; Roh, Kyoung Ho; Lee, Kyungwon; Yamane, Kunikazu; Arakawa, Yoshichika; Chong, Yunsop.

In: Diagnostic Microbiology and Infectious Disease, Vol. 56, No. 3, 01.11.2006, p. 305-312.

Research output: Contribution to journalArticle

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