Novel 16S rRNA methylase-mediated high-level resistance to amikacin and arbekacin has been reported recently in clinical isolates of Gram-negative bacilli only from several countries. We tested amikacin- or arbekacin-nonsusceptible Gram-negative bacilli isolated in 2003 and 2005 at a tertiary-care hospital in Korea by polymerase chain reaction to detect 16S rRNA methylase genes. armA alleles were detected in 14 isolates of Klebsiella pneumoniae, 10 other species of Enterobacteriaceae, and 16 Acinetobacter baumannii, whereas the rmtB allele was detected in 1 K. pneumoniae isolate. The resistance 1st detected in 2003 persisted in 2005. 16S rRNA methylase-producing isolates were highly resistant to arbekacin and amikacin, and were mostly coresistant to levofloxacin. Most K. pneumoniae isolates also produced extended-spectrum β-lactamases and plasmid-mediated AmpC β-lactamases, and most A. baumannii isolates were nonsusceptible to carbapenems.
|Number of pages||8|
|Journal||Diagnostic Microbiology and Infectious Disease|
|Publication status||Published - 2006 Nov|
Bibliographical noteFunding Information:
This work was supported in part by the Brain Korea 21 Project for Medical Sciences, Yonsei University, Seoul, South Korea, in 2004. The authors thank Myungsook Kim for collecting the isolates and Younghee Seon and Chasoon Lee for technical assistance.
All Science Journal Classification (ASJC) codes
- Microbiology (medical)
- Infectious Diseases