Dissemination of metallo-β-lactamase-producing Pseudomonas aeruginosa of sequence type 235 in Asian countries

Moon Jung Kim, Il Kwon Bae, Seokhoon Jeong, So Hyun Kim, Jae Hoon Song, Jae Young Choi, Sang Sun Yoon, Visanu Thamlikitkul, Po Ren Hsueh, Rohani Md Yasin, M. K. Lalitha, Kyungwon Lee

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Abstract

Objectives: To investigate the epidemiological traits of metallo-b-lactamase (MBL)-producing Pseudomonas aeruginosa (MPPA) clinical isolates collected by the Asian Network for Surveillance of Resistant Pathogens (ANSORP). Methods:Atotal of16MPPAclinical isolateswerecollected fromsix Asian countries in2000to2009byANSORP.The MBLgenewasdetected byPCRamplification. The genetic organization of the class1integroncarrying theMBLgene cassettewas investigated by PCR mapping and sequencing. Southern blotting, repetitive sequence-based PCR and multilocus sequence typing (MLST) experiments were performed to characterize the isolates. Results: PCR and sequencing experiments detected the bla VIM-2 (n=12), bla VIM-3 (n=1), bla IMP-6 (n=2) and bla IMP-26 (n=1) genes. The MBL genes were located on the chromosome in all isolates except one. Furthermore, all the MBL genes were located in a class 1 integron. All the MPPA isolates from Malaysia, Thailand, Sri Lanka and Korea were identified as sequence type (ST) 235 by MLST. Three VIM-2-producing isolates fromIndia were identified as ST773, and one isolate harbouring VIM-3 from Taiwan was identified as ST298. Conclusions: P. aeruginosa ST235might play a role in dissemination of MBL genes in Asian countries.

Original languageEnglish
Article numberdkt269
Pages (from-to)2820-2824
Number of pages5
JournalJournal of Antimicrobial Chemotherapy
Volume68
Issue number12
DOIs
Publication statusPublished - 2013 Dec 1

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All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Microbiology (medical)
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Kim, M. J., Bae, I. K., Jeong, S., Kim, S. H., Song, J. H., Choi, J. Y., Yoon, S. S., Thamlikitkul, V., Hsueh, P. R., Yasin, R. M., Lalitha, M. K., & Lee, K. (2013). Dissemination of metallo-β-lactamase-producing Pseudomonas aeruginosa of sequence type 235 in Asian countries. Journal of Antimicrobial Chemotherapy, 68(12), 2820-2824. [dkt269]. https://doi.org/10.1093/jac/dkt269