Dissemination of metallo-β-lactamase-producing Pseudomonas aeruginosa of sequence type 235 in Asian countries

Moon Jung Kim; Il Kwon Bae; Seok Hoon Jeong; So Hyun Kim; Jae Hoon Song; Jae Young Choi; Sang Sun Yoon; Visanu Thamlikitkul; Po Ren Hsueh; Rohani Md Yasin; M. K. Lalitha; Kyungwon Lee

doi:10.1093/jac/dkt269

Dissemination of metallo-β-lactamase-producing Pseudomonas aeruginosa of sequence type 235 in Asian countries

Moon Jung Kim, Il Kwon Bae, Seok Hoon Jeong, So Hyun Kim, Jae Hoon Song, Jae Young Choi, Sang Sun Yoon, Visanu Thamlikitkul, Po Ren Hsueh, Rohani Md Yasin, M. K. Lalitha, Kyungwon Lee

Department of Laboratory Medicine

Research output: Contribution to journal › Article › peer-review

50 Citations (Scopus)

Abstract

Objectives: To investigate the epidemiological traits of metallo-b-lactamase (MBL)-producing Pseudomonas aeruginosa (MPPA) clinical isolates collected by the Asian Network for Surveillance of Resistant Pathogens (ANSORP). Methods:Atotal of16MPPAclinical isolateswerecollected fromsix Asian countries in2000to2009byANSORP.The MBLgenewasdetected byPCRamplification. The genetic organization of the class1integroncarrying theMBLgene cassettewas investigated by PCR mapping and sequencing. Southern blotting, repetitive sequence-based PCR and multilocus sequence typing (MLST) experiments were performed to characterize the isolates. Results: PCR and sequencing experiments detected the bla_VIM-2 (n=12), bla_VIM-3 (n=1), bla_IMP-6 (n=2) and bla_IMP-26 (n=1) genes. The MBL genes were located on the chromosome in all isolates except one. Furthermore, all the MBL genes were located in a class 1 integron. All the MPPA isolates from Malaysia, Thailand, Sri Lanka and Korea were identified as sequence type (ST) 235 by MLST. Three VIM-2-producing isolates fromIndia were identified as ST773, and one isolate harbouring VIM-3 from Taiwan was identified as ST298. Conclusions: P. aeruginosa ST235might play a role in dissemination of MBL genes in Asian countries.

Original language	English
Article number	dkt269
Pages (from-to)	2820-2824
Number of pages	5
Journal	Journal of Antimicrobial Chemotherapy
Volume	68
Issue number	12
DOIs	https://doi.org/10.1093/jac/dkt269
Publication status	Published - 2013 Dec

Bibliographical note

Funding Information:
This study was supported by a grant from the Korea Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (A110096).

All Science Journal Classification (ASJC) codes

Pharmacology
Microbiology (medical)
Infectious Diseases
Pharmacology (medical)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1093/jac/dkt269

Cite this

Kim, M. J., Bae, I. K., Jeong, S. H., Kim, S. H., Song, J. H., Choi, J. Y., Yoon, S. S., Thamlikitkul, V., Hsueh, P. R., Yasin, R. M., Lalitha, M. K., & Lee, K. (2013). Dissemination of metallo-β-lactamase-producing Pseudomonas aeruginosa of sequence type 235 in Asian countries. Journal of Antimicrobial Chemotherapy, 68(12), 2820-2824. [dkt269]. https://doi.org/10.1093/jac/dkt269

@article{e37d2676f57e4c21ba177c5d91aa42dc,

title = "Dissemination of metallo-β-lactamase-producing Pseudomonas aeruginosa of sequence type 235 in Asian countries",

abstract = "Objectives: To investigate the epidemiological traits of metallo-b-lactamase (MBL)-producing Pseudomonas aeruginosa (MPPA) clinical isolates collected by the Asian Network for Surveillance of Resistant Pathogens (ANSORP). Methods:Atotal of16MPPAclinical isolateswerecollected fromsix Asian countries in2000to2009byANSORP.The MBLgenewasdetected byPCRamplification. The genetic organization of the class1integroncarrying theMBLgene cassettewas investigated by PCR mapping and sequencing. Southern blotting, repetitive sequence-based PCR and multilocus sequence typing (MLST) experiments were performed to characterize the isolates. Results: PCR and sequencing experiments detected the blaVIM-2 (n=12), blaVIM-3 (n=1), blaIMP-6 (n=2) and blaIMP-26 (n=1) genes. The MBL genes were located on the chromosome in all isolates except one. Furthermore, all the MBL genes were located in a class 1 integron. All the MPPA isolates from Malaysia, Thailand, Sri Lanka and Korea were identified as sequence type (ST) 235 by MLST. Three VIM-2-producing isolates fromIndia were identified as ST773, and one isolate harbouring VIM-3 from Taiwan was identified as ST298. Conclusions: P. aeruginosa ST235might play a role in dissemination of MBL genes in Asian countries.",

author = "Kim, {Moon Jung} and Bae, {Il Kwon} and Jeong, {Seok Hoon} and Kim, {So Hyun} and Song, {Jae Hoon} and Choi, {Jae Young} and Yoon, {Sang Sun} and Visanu Thamlikitkul and Hsueh, {Po Ren} and Yasin, {Rohani Md} and Lalitha, {M. K.} and Kyungwon Lee",

note = "Funding Information: This study was supported by a grant from the Korea Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (A110096).",

year = "2013",

month = dec,

doi = "10.1093/jac/dkt269",

language = "English",

volume = "68",

pages = "2820--2824",

journal = "Journal of Antimicrobial Chemotherapy",

issn = "0305-7453",

publisher = "Oxford University Press",

number = "12",

}

TY - JOUR

T1 - Dissemination of metallo-β-lactamase-producing Pseudomonas aeruginosa of sequence type 235 in Asian countries

AU - Kim, Moon Jung

AU - Bae, Il Kwon

AU - Jeong, Seok Hoon

AU - Kim, So Hyun

AU - Song, Jae Hoon

AU - Choi, Jae Young

AU - Yoon, Sang Sun

AU - Thamlikitkul, Visanu

AU - Hsueh, Po Ren

AU - Yasin, Rohani Md

AU - Lalitha, M. K.

AU - Lee, Kyungwon

N1 - Funding Information: This study was supported by a grant from the Korea Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (A110096).

PY - 2013/12

Y1 - 2013/12

N2 - Objectives: To investigate the epidemiological traits of metallo-b-lactamase (MBL)-producing Pseudomonas aeruginosa (MPPA) clinical isolates collected by the Asian Network for Surveillance of Resistant Pathogens (ANSORP). Methods:Atotal of16MPPAclinical isolateswerecollected fromsix Asian countries in2000to2009byANSORP.The MBLgenewasdetected byPCRamplification. The genetic organization of the class1integroncarrying theMBLgene cassettewas investigated by PCR mapping and sequencing. Southern blotting, repetitive sequence-based PCR and multilocus sequence typing (MLST) experiments were performed to characterize the isolates. Results: PCR and sequencing experiments detected the blaVIM-2 (n=12), blaVIM-3 (n=1), blaIMP-6 (n=2) and blaIMP-26 (n=1) genes. The MBL genes were located on the chromosome in all isolates except one. Furthermore, all the MBL genes were located in a class 1 integron. All the MPPA isolates from Malaysia, Thailand, Sri Lanka and Korea were identified as sequence type (ST) 235 by MLST. Three VIM-2-producing isolates fromIndia were identified as ST773, and one isolate harbouring VIM-3 from Taiwan was identified as ST298. Conclusions: P. aeruginosa ST235might play a role in dissemination of MBL genes in Asian countries.

AB - Objectives: To investigate the epidemiological traits of metallo-b-lactamase (MBL)-producing Pseudomonas aeruginosa (MPPA) clinical isolates collected by the Asian Network for Surveillance of Resistant Pathogens (ANSORP). Methods:Atotal of16MPPAclinical isolateswerecollected fromsix Asian countries in2000to2009byANSORP.The MBLgenewasdetected byPCRamplification. The genetic organization of the class1integroncarrying theMBLgene cassettewas investigated by PCR mapping and sequencing. Southern blotting, repetitive sequence-based PCR and multilocus sequence typing (MLST) experiments were performed to characterize the isolates. Results: PCR and sequencing experiments detected the blaVIM-2 (n=12), blaVIM-3 (n=1), blaIMP-6 (n=2) and blaIMP-26 (n=1) genes. The MBL genes were located on the chromosome in all isolates except one. Furthermore, all the MBL genes were located in a class 1 integron. All the MPPA isolates from Malaysia, Thailand, Sri Lanka and Korea were identified as sequence type (ST) 235 by MLST. Three VIM-2-producing isolates fromIndia were identified as ST773, and one isolate harbouring VIM-3 from Taiwan was identified as ST298. Conclusions: P. aeruginosa ST235might play a role in dissemination of MBL genes in Asian countries.

UR - http://www.scopus.com/inward/record.url?scp=84887517155&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84887517155&partnerID=8YFLogxK

U2 - 10.1093/jac/dkt269

DO - 10.1093/jac/dkt269

M3 - Article

C2 - 23843299

AN - SCOPUS:84887517155

SN - 0305-7453

VL - 68

SP - 2820

EP - 2824

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

IS - 12

M1 - dkt269

ER -

Dissemination of metallo-β-lactamase-producing Pseudomonas aeruginosa of sequence type 235 in Asian countries

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this