Dissemination of metallo-β-lactamase-producing Pseudomonas aeruginosa of sequence type 235 in Asian countries

Moon Jung Kim, Il Kwon Bae, Seokhoon Jeong, So Hyun Kim, Jae Hoon Song, Jae Young Choi, Sang Sun Yoon, Visanu Thamlikitkul, Po Ren Hsueh, Rohani Md Yasin, M. K. Lalitha, Kyungwon Lee

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Objectives: To investigate the epidemiological traits of metallo-b-lactamase (MBL)-producing Pseudomonas aeruginosa (MPPA) clinical isolates collected by the Asian Network for Surveillance of Resistant Pathogens (ANSORP). Methods:Atotal of16MPPAclinical isolateswerecollected fromsix Asian countries in2000to2009byANSORP.The MBLgenewasdetected byPCRamplification. The genetic organization of the class1integroncarrying theMBLgene cassettewas investigated by PCR mapping and sequencing. Southern blotting, repetitive sequence-based PCR and multilocus sequence typing (MLST) experiments were performed to characterize the isolates. Results: PCR and sequencing experiments detected the bla VIM-2 (n=12), bla VIM-3 (n=1), bla IMP-6 (n=2) and bla IMP-26 (n=1) genes. The MBL genes were located on the chromosome in all isolates except one. Furthermore, all the MBL genes were located in a class 1 integron. All the MPPA isolates from Malaysia, Thailand, Sri Lanka and Korea were identified as sequence type (ST) 235 by MLST. Three VIM-2-producing isolates fromIndia were identified as ST773, and one isolate harbouring VIM-3 from Taiwan was identified as ST298. Conclusions: P. aeruginosa ST235might play a role in dissemination of MBL genes in Asian countries.

Original languageEnglish
Article numberdkt269
Pages (from-to)2820-2824
Number of pages5
JournalJournal of Antimicrobial Chemotherapy
Volume68
Issue number12
DOIs
Publication statusPublished - 2013 Dec 1

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Pseudomonas aeruginosa
Multilocus Sequence Typing
Inosine Monophosphate
Polymerase Chain Reaction
Genes
Integrons
Sri Lanka
Nucleic Acid Repetitive Sequences
Malaysia
Thailand
Korea
Southern Blotting
Taiwan
Chromosomes

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Microbiology (medical)
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Kim, Moon Jung ; Bae, Il Kwon ; Jeong, Seokhoon ; Kim, So Hyun ; Song, Jae Hoon ; Choi, Jae Young ; Yoon, Sang Sun ; Thamlikitkul, Visanu ; Hsueh, Po Ren ; Yasin, Rohani Md ; Lalitha, M. K. ; Lee, Kyungwon. / Dissemination of metallo-β-lactamase-producing Pseudomonas aeruginosa of sequence type 235 in Asian countries. In: Journal of Antimicrobial Chemotherapy. 2013 ; Vol. 68, No. 12. pp. 2820-2824.
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abstract = "Objectives: To investigate the epidemiological traits of metallo-b-lactamase (MBL)-producing Pseudomonas aeruginosa (MPPA) clinical isolates collected by the Asian Network for Surveillance of Resistant Pathogens (ANSORP). Methods:Atotal of16MPPAclinical isolateswerecollected fromsix Asian countries in2000to2009byANSORP.The MBLgenewasdetected byPCRamplification. The genetic organization of the class1integroncarrying theMBLgene cassettewas investigated by PCR mapping and sequencing. Southern blotting, repetitive sequence-based PCR and multilocus sequence typing (MLST) experiments were performed to characterize the isolates. Results: PCR and sequencing experiments detected the bla VIM-2 (n=12), bla VIM-3 (n=1), bla IMP-6 (n=2) and bla IMP-26 (n=1) genes. The MBL genes were located on the chromosome in all isolates except one. Furthermore, all the MBL genes were located in a class 1 integron. All the MPPA isolates from Malaysia, Thailand, Sri Lanka and Korea were identified as sequence type (ST) 235 by MLST. Three VIM-2-producing isolates fromIndia were identified as ST773, and one isolate harbouring VIM-3 from Taiwan was identified as ST298. Conclusions: P. aeruginosa ST235might play a role in dissemination of MBL genes in Asian countries.",
author = "Kim, {Moon Jung} and Bae, {Il Kwon} and Seokhoon Jeong and Kim, {So Hyun} and Song, {Jae Hoon} and Choi, {Jae Young} and Yoon, {Sang Sun} and Visanu Thamlikitkul and Hsueh, {Po Ren} and Yasin, {Rohani Md} and Lalitha, {M. K.} and Kyungwon Lee",
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Kim, MJ, Bae, IK, Jeong, S, Kim, SH, Song, JH, Choi, JY, Yoon, SS, Thamlikitkul, V, Hsueh, PR, Yasin, RM, Lalitha, MK & Lee, K 2013, 'Dissemination of metallo-β-lactamase-producing Pseudomonas aeruginosa of sequence type 235 in Asian countries', Journal of Antimicrobial Chemotherapy, vol. 68, no. 12, dkt269, pp. 2820-2824. https://doi.org/10.1093/jac/dkt269

Dissemination of metallo-β-lactamase-producing Pseudomonas aeruginosa of sequence type 235 in Asian countries. / Kim, Moon Jung; Bae, Il Kwon; Jeong, Seokhoon; Kim, So Hyun; Song, Jae Hoon; Choi, Jae Young; Yoon, Sang Sun; Thamlikitkul, Visanu; Hsueh, Po Ren; Yasin, Rohani Md; Lalitha, M. K.; Lee, Kyungwon.

In: Journal of Antimicrobial Chemotherapy, Vol. 68, No. 12, dkt269, 01.12.2013, p. 2820-2824.

Research output: Contribution to journalArticle

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T1 - Dissemination of metallo-β-lactamase-producing Pseudomonas aeruginosa of sequence type 235 in Asian countries

AU - Kim, Moon Jung

AU - Bae, Il Kwon

AU - Jeong, Seokhoon

AU - Kim, So Hyun

AU - Song, Jae Hoon

AU - Choi, Jae Young

AU - Yoon, Sang Sun

AU - Thamlikitkul, Visanu

AU - Hsueh, Po Ren

AU - Yasin, Rohani Md

AU - Lalitha, M. K.

AU - Lee, Kyungwon

PY - 2013/12/1

Y1 - 2013/12/1

N2 - Objectives: To investigate the epidemiological traits of metallo-b-lactamase (MBL)-producing Pseudomonas aeruginosa (MPPA) clinical isolates collected by the Asian Network for Surveillance of Resistant Pathogens (ANSORP). Methods:Atotal of16MPPAclinical isolateswerecollected fromsix Asian countries in2000to2009byANSORP.The MBLgenewasdetected byPCRamplification. The genetic organization of the class1integroncarrying theMBLgene cassettewas investigated by PCR mapping and sequencing. Southern blotting, repetitive sequence-based PCR and multilocus sequence typing (MLST) experiments were performed to characterize the isolates. Results: PCR and sequencing experiments detected the bla VIM-2 (n=12), bla VIM-3 (n=1), bla IMP-6 (n=2) and bla IMP-26 (n=1) genes. The MBL genes were located on the chromosome in all isolates except one. Furthermore, all the MBL genes were located in a class 1 integron. All the MPPA isolates from Malaysia, Thailand, Sri Lanka and Korea were identified as sequence type (ST) 235 by MLST. Three VIM-2-producing isolates fromIndia were identified as ST773, and one isolate harbouring VIM-3 from Taiwan was identified as ST298. Conclusions: P. aeruginosa ST235might play a role in dissemination of MBL genes in Asian countries.

AB - Objectives: To investigate the epidemiological traits of metallo-b-lactamase (MBL)-producing Pseudomonas aeruginosa (MPPA) clinical isolates collected by the Asian Network for Surveillance of Resistant Pathogens (ANSORP). Methods:Atotal of16MPPAclinical isolateswerecollected fromsix Asian countries in2000to2009byANSORP.The MBLgenewasdetected byPCRamplification. The genetic organization of the class1integroncarrying theMBLgene cassettewas investigated by PCR mapping and sequencing. Southern blotting, repetitive sequence-based PCR and multilocus sequence typing (MLST) experiments were performed to characterize the isolates. Results: PCR and sequencing experiments detected the bla VIM-2 (n=12), bla VIM-3 (n=1), bla IMP-6 (n=2) and bla IMP-26 (n=1) genes. The MBL genes were located on the chromosome in all isolates except one. Furthermore, all the MBL genes were located in a class 1 integron. All the MPPA isolates from Malaysia, Thailand, Sri Lanka and Korea were identified as sequence type (ST) 235 by MLST. Three VIM-2-producing isolates fromIndia were identified as ST773, and one isolate harbouring VIM-3 from Taiwan was identified as ST298. Conclusions: P. aeruginosa ST235might play a role in dissemination of MBL genes in Asian countries.

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