Dissemination of SHV-12 and characterization of new AmpC-type beta-lactamase genes among clinical isolates of Enterobacter species in Korea

Sang Hee Lee, Jae Young Kim, Sang Heum Shin, Young Jun An, Young Wook Choi, Yeun Chang Jung, Ha Il Jung, Eui Suk Sohn, Seokhoon Jeong, Kye Joon Lee

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

To determine the prevalence and genotype of an extended-spectrum beta-lactamase and new chromosomal AmpC beta-lactamases among clinical isolates of Enterobacter species, we performed antibiotic susceptibility testing, pI determination, induction tests, transconjugation, enterobacterial repetitive consensus (ERIC) PCR, sequencing, and phylogenetic analysis. Among the 51 clinical isolates collected from a university hospital in Korea, 6 isolates have been shown to produce SHV-12 and inducible AmpC beta-lactamases. These also included three isolates producing TEM-1b and one strain carrying TEM-1b and CMY-type beta-lactamases with a pI of 8.0. The results from ERIC PCR revealed that six isolates were genetically unrelated, suggesting that dissemination of SHV-12 was responsible for the spread of resistance to extended-spectrum beta-lactams in Korea. Six genes of inducible AmpC beta-lactamases that are responsible for the resistance to cephamycins (cefoxitin and cefotetan), amoxicillin, cephalothin, and amoxicillin-clavulanic acid were cloned and characterized. A 1,165-bp DNA fragment containing the ampC genes was sequenced and found to have an open reading frame coding for a 381-amino-acid beta-lactamase. The nucleotide sequence of four ampC genes (bla EcloK992004.1 , bla EcloK995120.1 , bla EcloK99230 , and bla EareK9911729 ) shared considerable homology with that of AmpC-type class C beta-lactamase genes of gram-negative bacteria, especially that of the chromosomal ampC gene (bla EcloMHN1 ) of Enterobacter cloacae MHN1 (99.9, 99.7, 99.6, and 99.6% identity, respectively). The sequences of two ampC genes (bla EcloK9973 and bla EcloK9914325 ) showed close similarity to the chromosomal ampC gene (bla EcloQ908R ) of E. cloacae Q908R (99.7% identity). The results from phylogenetic analysis suggested that six ampC genes could originate from bla EcloMHN1 or bla EcloQ908R .

Original languageEnglish
Pages (from-to)2477-2482
Number of pages6
JournalJournal of Clinical Microbiology
Volume41
Issue number6
DOIs
Publication statusPublished - 2003 Jun 1

Fingerprint

Enterobacter
Korea
beta-Lactamases
Genes
Enterobacter cloacae
Cephamycins
Cefotetan
Cephalothin
Amoxicillin-Potassium Clavulanate Combination
Cefoxitin
Polymerase Chain Reaction
AmpC beta-lactamases
Amoxicillin
beta-Lactams
Gram-Negative Bacteria
Open Reading Frames
Genotype
Anti-Bacterial Agents
Amino Acids
DNA

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

Lee, Sang Hee ; Kim, Jae Young ; Shin, Sang Heum ; An, Young Jun ; Choi, Young Wook ; Jung, Yeun Chang ; Jung, Ha Il ; Sohn, Eui Suk ; Jeong, Seokhoon ; Lee, Kye Joon. / Dissemination of SHV-12 and characterization of new AmpC-type beta-lactamase genes among clinical isolates of Enterobacter species in Korea. In: Journal of Clinical Microbiology. 2003 ; Vol. 41, No. 6. pp. 2477-2482.
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Dissemination of SHV-12 and characterization of new AmpC-type beta-lactamase genes among clinical isolates of Enterobacter species in Korea. / Lee, Sang Hee; Kim, Jae Young; Shin, Sang Heum; An, Young Jun; Choi, Young Wook; Jung, Yeun Chang; Jung, Ha Il; Sohn, Eui Suk; Jeong, Seokhoon; Lee, Kye Joon.

In: Journal of Clinical Microbiology, Vol. 41, No. 6, 01.06.2003, p. 2477-2482.

Research output: Contribution to journalArticle

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AU - Lee, Sang Hee

AU - Kim, Jae Young

AU - Shin, Sang Heum

AU - An, Young Jun

AU - Choi, Young Wook

AU - Jung, Yeun Chang

AU - Jung, Ha Il

AU - Sohn, Eui Suk

AU - Jeong, Seokhoon

AU - Lee, Kye Joon

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N2 - To determine the prevalence and genotype of an extended-spectrum beta-lactamase and new chromosomal AmpC beta-lactamases among clinical isolates of Enterobacter species, we performed antibiotic susceptibility testing, pI determination, induction tests, transconjugation, enterobacterial repetitive consensus (ERIC) PCR, sequencing, and phylogenetic analysis. Among the 51 clinical isolates collected from a university hospital in Korea, 6 isolates have been shown to produce SHV-12 and inducible AmpC beta-lactamases. These also included three isolates producing TEM-1b and one strain carrying TEM-1b and CMY-type beta-lactamases with a pI of 8.0. The results from ERIC PCR revealed that six isolates were genetically unrelated, suggesting that dissemination of SHV-12 was responsible for the spread of resistance to extended-spectrum beta-lactams in Korea. Six genes of inducible AmpC beta-lactamases that are responsible for the resistance to cephamycins (cefoxitin and cefotetan), amoxicillin, cephalothin, and amoxicillin-clavulanic acid were cloned and characterized. A 1,165-bp DNA fragment containing the ampC genes was sequenced and found to have an open reading frame coding for a 381-amino-acid beta-lactamase. The nucleotide sequence of four ampC genes (bla EcloK992004.1 , bla EcloK995120.1 , bla EcloK99230 , and bla EareK9911729 ) shared considerable homology with that of AmpC-type class C beta-lactamase genes of gram-negative bacteria, especially that of the chromosomal ampC gene (bla EcloMHN1 ) of Enterobacter cloacae MHN1 (99.9, 99.7, 99.6, and 99.6% identity, respectively). The sequences of two ampC genes (bla EcloK9973 and bla EcloK9914325 ) showed close similarity to the chromosomal ampC gene (bla EcloQ908R ) of E. cloacae Q908R (99.7% identity). The results from phylogenetic analysis suggested that six ampC genes could originate from bla EcloMHN1 or bla EcloQ908R .

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