Dissemination of SHV-12 and CTX-M-type extended-spectrum β-lactamases among clinical isolates of Escherichia coli and Klebsiella pneumoniae and emergence of GES-3 in Korea

Nam Hee Ryoo, Eui Chong Kim, Seong Geun Hong, Yeon Joon Park, Kyungwon Lee, Il Kwon Bae, Eun Hyang Song, Seokhoon Jeong

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

Objectives: To assess the prevalence and genotypes of Ambler class A extended-spectrum β-lactamases (ESBLs) in Korea. Methods: Clinical isolates of Escherichia coli and Klebsiella pneumoniae collected from 12 Korean hospitals during February-July 2003 were evaluated. Antimicrobial susceptibilities were determined by disc diffusion and agar dilution methods, and the putative ESBL-producing strains were tested by the double-disc synergy method. Detection of genes encoding class A β-lactamases was performed by PCR amplification, and the PCR products were subjected to direct sequencing. Results: The double-disc synergy test showed positive results in 9.3% (23/246) of E. coli and 23.0% (55/239) of K. pneumoniae isolates. The most prevalent types of Ambler class A ESBLs in E. coli isolates were CTX-M-15 (n=4) and CTX-M-3 (n=3), and those in K. pneumoniae isolates were SHV-12 (n=30) and CTX-M-3 (n=13). Two isolates produced both SHV-12 and GES-3, simultaneously. Conclusions: CTX-M-type and/or SHV-12 ESBL-producing E. coli and K. pneumoniae isolates are spreading, and a GES-type ESBL has emerged in Korea.

Original languageEnglish
Pages (from-to)698-702
Number of pages5
JournalJournal of Antimicrobial Chemotherapy
Volume56
Issue number4
DOIs
Publication statusPublished - 2005 Oct 1

Fingerprint

Klebsiella pneumoniae
Korea
Escherichia coli
Polymerase Chain Reaction
Agar
Genotype
beta-lactamase CTX-2
Genes

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Microbiology

Cite this

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title = "Dissemination of SHV-12 and CTX-M-type extended-spectrum β-lactamases among clinical isolates of Escherichia coli and Klebsiella pneumoniae and emergence of GES-3 in Korea",
abstract = "Objectives: To assess the prevalence and genotypes of Ambler class A extended-spectrum β-lactamases (ESBLs) in Korea. Methods: Clinical isolates of Escherichia coli and Klebsiella pneumoniae collected from 12 Korean hospitals during February-July 2003 were evaluated. Antimicrobial susceptibilities were determined by disc diffusion and agar dilution methods, and the putative ESBL-producing strains were tested by the double-disc synergy method. Detection of genes encoding class A β-lactamases was performed by PCR amplification, and the PCR products were subjected to direct sequencing. Results: The double-disc synergy test showed positive results in 9.3{\%} (23/246) of E. coli and 23.0{\%} (55/239) of K. pneumoniae isolates. The most prevalent types of Ambler class A ESBLs in E. coli isolates were CTX-M-15 (n=4) and CTX-M-3 (n=3), and those in K. pneumoniae isolates were SHV-12 (n=30) and CTX-M-3 (n=13). Two isolates produced both SHV-12 and GES-3, simultaneously. Conclusions: CTX-M-type and/or SHV-12 ESBL-producing E. coli and K. pneumoniae isolates are spreading, and a GES-type ESBL has emerged in Korea.",
author = "Ryoo, {Nam Hee} and Kim, {Eui Chong} and Hong, {Seong Geun} and Park, {Yeon Joon} and Kyungwon Lee and Bae, {Il Kwon} and Song, {Eun Hyang} and Seokhoon Jeong",
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Dissemination of SHV-12 and CTX-M-type extended-spectrum β-lactamases among clinical isolates of Escherichia coli and Klebsiella pneumoniae and emergence of GES-3 in Korea. / Ryoo, Nam Hee; Kim, Eui Chong; Hong, Seong Geun; Park, Yeon Joon; Lee, Kyungwon; Bae, Il Kwon; Song, Eun Hyang; Jeong, Seokhoon.

In: Journal of Antimicrobial Chemotherapy, Vol. 56, No. 4, 01.10.2005, p. 698-702.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Dissemination of SHV-12 and CTX-M-type extended-spectrum β-lactamases among clinical isolates of Escherichia coli and Klebsiella pneumoniae and emergence of GES-3 in Korea

AU - Ryoo, Nam Hee

AU - Kim, Eui Chong

AU - Hong, Seong Geun

AU - Park, Yeon Joon

AU - Lee, Kyungwon

AU - Bae, Il Kwon

AU - Song, Eun Hyang

AU - Jeong, Seokhoon

PY - 2005/10/1

Y1 - 2005/10/1

N2 - Objectives: To assess the prevalence and genotypes of Ambler class A extended-spectrum β-lactamases (ESBLs) in Korea. Methods: Clinical isolates of Escherichia coli and Klebsiella pneumoniae collected from 12 Korean hospitals during February-July 2003 were evaluated. Antimicrobial susceptibilities were determined by disc diffusion and agar dilution methods, and the putative ESBL-producing strains were tested by the double-disc synergy method. Detection of genes encoding class A β-lactamases was performed by PCR amplification, and the PCR products were subjected to direct sequencing. Results: The double-disc synergy test showed positive results in 9.3% (23/246) of E. coli and 23.0% (55/239) of K. pneumoniae isolates. The most prevalent types of Ambler class A ESBLs in E. coli isolates were CTX-M-15 (n=4) and CTX-M-3 (n=3), and those in K. pneumoniae isolates were SHV-12 (n=30) and CTX-M-3 (n=13). Two isolates produced both SHV-12 and GES-3, simultaneously. Conclusions: CTX-M-type and/or SHV-12 ESBL-producing E. coli and K. pneumoniae isolates are spreading, and a GES-type ESBL has emerged in Korea.

AB - Objectives: To assess the prevalence and genotypes of Ambler class A extended-spectrum β-lactamases (ESBLs) in Korea. Methods: Clinical isolates of Escherichia coli and Klebsiella pneumoniae collected from 12 Korean hospitals during February-July 2003 were evaluated. Antimicrobial susceptibilities were determined by disc diffusion and agar dilution methods, and the putative ESBL-producing strains were tested by the double-disc synergy method. Detection of genes encoding class A β-lactamases was performed by PCR amplification, and the PCR products were subjected to direct sequencing. Results: The double-disc synergy test showed positive results in 9.3% (23/246) of E. coli and 23.0% (55/239) of K. pneumoniae isolates. The most prevalent types of Ambler class A ESBLs in E. coli isolates were CTX-M-15 (n=4) and CTX-M-3 (n=3), and those in K. pneumoniae isolates were SHV-12 (n=30) and CTX-M-3 (n=13). Two isolates produced both SHV-12 and GES-3, simultaneously. Conclusions: CTX-M-type and/or SHV-12 ESBL-producing E. coli and K. pneumoniae isolates are spreading, and a GES-type ESBL has emerged in Korea.

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SN - 0305-7453

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