Dissemination of SHV-12 and CTX-M-type extended-spectrum β-lactamases among clinical isolates of Escherichia coli and Klebsiella pneumoniae and emergence of GES-3 in Korea

Nam Hee Ryoo, Eui Chong Kim, Seong Geun Hong, Yeon Joon Park, Kyungwon Lee, Il Kwon Bae, Eun Hyang Song, Seok Hoon Jeong

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Objectives: To assess the prevalence and genotypes of Ambler class A extended-spectrum β-lactamases (ESBLs) in Korea. Methods: Clinical isolates of Escherichia coli and Klebsiella pneumoniae collected from 12 Korean hospitals during February-July 2003 were evaluated. Antimicrobial susceptibilities were determined by disc diffusion and agar dilution methods, and the putative ESBL-producing strains were tested by the double-disc synergy method. Detection of genes encoding class A β-lactamases was performed by PCR amplification, and the PCR products were subjected to direct sequencing. Results: The double-disc synergy test showed positive results in 9.3% (23/246) of E. coli and 23.0% (55/239) of K. pneumoniae isolates. The most prevalent types of Ambler class A ESBLs in E. coli isolates were CTX-M-15 (n=4) and CTX-M-3 (n=3), and those in K. pneumoniae isolates were SHV-12 (n=30) and CTX-M-3 (n=13). Two isolates produced both SHV-12 and GES-3, simultaneously. Conclusions: CTX-M-type and/or SHV-12 ESBL-producing E. coli and K. pneumoniae isolates are spreading, and a GES-type ESBL has emerged in Korea.

Original languageEnglish
Pages (from-to)698-702
Number of pages5
JournalJournal of Antimicrobial Chemotherapy
Issue number4
Publication statusPublished - 2005 Oct 1


All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

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